Nitrogen and sulfur co-doped carbon dot-based ratiometric fluorescent probe for Zn2+ sensing and imaging in living cells

Chen, Shengyu; Li, Suping; Liu, Xiaofeng; Shi, Bingfang; Huang, Yijun; Zhao, Shulin

doi:10.1007/s00604-022-05188-7

Cited by 13 publications

(5 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, the fluorescence emission at 649 nm was very sensitive to Zn 2+ . The enhancement factor was 13.3, much more than that of the previous CDs [36]. The fluorescence quantum yield of the CDs was calculated to be 14.09%.…”

Section: Colorimetric Fluorescence Detection Of Zinc Ionmentioning

confidence: 72%

A Ratiometric Fluorescent Sensor Based on Chelation-Enhanced Fluorescence of Carbon Dots for Zinc Ion Detection

Lu,

Jia,

et al. 2023

Molecules

View full text Add to dashboard Cite

Zinc ion, one of the most important transition metal ions in living organisms, plays a crucial role in the homeostasis of the organism. The disorder of zinc is associated with many major diseases. It is highly desirable to develop selective and sensitive methods for the real-time detection of zinc ions. In this work, double-emitting fluorescent carbon dots (CDs) are prepared by a solvothermal method using glutathione, L-aspartic acid, and formamide as the raw materials. The carbon dots specifically recognize zine ions and produce a decrease in fluorescence intensity at 684 nm and an increase at 649 nm, leading to a ratiometric fluorescent sensor for zinc detection. Through surface modification and spectral analysis, the surface groups including carboxyl, carbonyl, hydroxyl, and amino groups, and C=N in heterocycles of CDs are revealed to synergistically coordinate Zn2+, inducing the structural changes in the emission site. The CDs can afford a low limit of detection of ~5 nM for Zn2+ detection with good linearity in the range of 0.02–5 μM, showing good selectivity as well. The results from real samples including fetal bovine serum, milk powder, and zinc gluconate oral solution indicated the good applicability of the CDs in the determination of Zn2+.

show abstract

Section: Colorimetric Fluorescence Detection Of Zinc Ionmentioning

confidence: 72%

A Ratiometric Fluorescent Sensor Based on Chelation-Enhanced Fluorescence of Carbon Dots for Zinc Ion Detection

Lu,

Jia,

et al. 2023

Molecules

View full text Add to dashboard Cite

show abstract

“…The dual emission strategy is a versatile and effective approach that capitalizes on the intrinsic fluorescence properties of CDs. In this method, CDs are engineered or modified to emit light at two distinct wavelengths under the excitation of a single wavelength [ 64 , 66 , 67 , 74 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 ] ( Figure 4 ). These dual-emitting CDs offer a built-in reference signal, enhancing the accuracy and reliability of ratiometric measurements.…”

Section: Construction Strategies Of Cds In Detecting Environmental Co...mentioning

confidence: 99%

“…This probe not only enabled precise quantification of Pb 2+ but also exhibited remarkable sensitivity, with a detection limit of Pb 2+ as low as 2.7 nM. Chen et al [ 99 ] introduced a near-infrared ratiometric fluorescent probe based on nitrogen and sulfur co-doped CDs (N, S-CDs). This probe was synthesized using a hydrothermal approach, employing glutathione and formamide as precursors.…”

Section: Construction Strategies Of Cds In Detecting Environmental Co...mentioning

confidence: 99%

Advances in Carbon Dot-Based Ratiometric Fluorescent Probes for Environmental Contaminant Detection: A Review

Xing,

Wang,

Wang

2024

Micromachines

View full text Add to dashboard Cite

Detecting environmental contaminants is crucial for protecting ecosystems and human health. While traditional carbon dot (CD) fluorescent probes are versatile, they may suffer from limitations like fluctuations in signal intensity, leading to detection inaccuracies. In contrast, ratiometric fluorescent probes, designed with internal self-calibration mechanisms, offer enhanced sensitivity and reliability. This review focuses on the design and applications of ratiometric fluorescent probes based on CDs for environmental monitoring. Our discussion covers construction strategies, ratiometric fluorescence principles, and applications in detecting various environmental contaminants, including organic pollutants, heavy metal ions, and other substances. We also explore associated advantages and challenges and provide insights into potential solutions and future research directions.

show abstract

“…Several strategies have been adopted to develop CD-based methods for probing zinc ion (Zn 2þ ) in cells. [234][235][236][237][238] Zhang et al prepared a CD-based Zn 2þ sensor by modifying the surface of CDs with a specific Zn 2þ recognition group (a quinoline derivative) (Figure 10A). [234] The CDs were synthesized by the lowtemperature pyrolysis of CA and branched PEI.…”

Section: Metal Ionsmentioning

confidence: 99%

Carbon Dots for Intracellular Sensing

Lin

Jia

2022

Small Structures

View full text Add to dashboard Cite

Carbon dots (CDs), a type of small, carbon element‐based nanomaterials, have found numerous applications in many fields due to their outstanding properties like fascinating photoluminescence characteristic, great biocompatibility, easy and economical synthesis, and facile functionalization. Over the last decade, huge progress and achievements have been made in terms of the applications of CDs in cellular detection. However, a comprehensive review focusing on this topic is still lacking. Herein, the recent progress in CDs‐mediated intracellular detection, including the fluorescence imaging of cellular structures (e.g., nucleus/nucleolus, mitochondrion, lysosome, endoplasmic reticulum, Golgi apparatus, and lipid droplet), the fluorescence detection of a large variety of cellular substances (including endogenous biomacromolecules and their monomers, vitamins, important metabolites, adenosine triphosphate, reactive oxygen species, biothiols, ions, and exogenous compounds), and the monitoring of cellular parameters (pH, temperature, and mitochondrial membrane potential), is thoroughly reviewed. The synthesis, functionalization, and performance of the CDs that serve as fluorescent nanoprobes in living cells are presented. Their underlying working mechanisms are also discussed if available. Finally, the challenges and future research directions of the related research field are listed. This review may foster the future development of more CDs with better cellular sensing performance.

show abstract

Nitrogen and sulfur co-doped carbon dot-based ratiometric fluorescent probe for Zn2+ sensing and imaging in living cells

Cited by 13 publications

References 33 publications

A Ratiometric Fluorescent Sensor Based on Chelation-Enhanced Fluorescence of Carbon Dots for Zinc Ion Detection

A Ratiometric Fluorescent Sensor Based on Chelation-Enhanced Fluorescence of Carbon Dots for Zinc Ion Detection

Advances in Carbon Dot-Based Ratiometric Fluorescent Probes for Environmental Contaminant Detection: A Review

Carbon Dots for Intracellular Sensing

Contact Info

Product

Resources

About