Nitrogen assimilation and gene regulation of two Kentucky bluegrass cultivars differing in response to nitrate supply

Sun, Xiaoyang; Zheng, Qianjiao; Xiong, Liangbing; Xie, Fang; Li, Xun; Li, Yong; Zhang, Lu; Saud, Shah; Guo, Zhixin; Yu, Yan; Wu, Hanfu; Liu, Qingqing; Cui, Guowen; Chen, Yajun

doi:10.1016/j.scienta.2021.110315

Cited by 8 publications

(28 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two Kentucky bluegrass cultivars (Bluemoon and Balin) were collected from field plots at the Horticulture Experimental Station of Northeast Agricultural University (Harbin, China; 128 • 04 E, 44 • 50 N) and were used for the experiment. Bluemoon was more tolerant to low N stress than Balin (Sun et al, 2021). Two-year-old turf plants were transplanted to pots (15 cm in diameter and 45 cm in height) filled with a mixture of sand and vermiculite (2:1) in a greenhouse.…”

Section: Plant Materials Growing Conditions and Experimental Designmentioning

confidence: 99%

“…Two weeks after adaptation to the growth chamber environment, the pots were rinsed with deionized water for 1 week and followed by two N treatments: low N (LN, 0.5 mM NO 3 − ) and normal N (NN, 15 mM NO 3 − ) with Hoagland solution used as the N source. The N rates were chosen based on our previous study that two cultivars differed in their responses to these two N treatments (Sun et al, 2021). The experiment was a completely randomized design with 2 × 2 factorial arrangements (two N treatments, two cultivars).…”

Section: Plant Materials Growing Conditions and Experimental Designmentioning

confidence: 99%

“…Plant responses to external N supply often result in many cellular and molecular changes related to N metabolism. For example, low N stress reduced leaf activities of glutamine synthetase and glutamate synthetase in Kentucky bluegrass, but lower activity of nitrate reductase was found only in the sensitive cultivar exposed to low N condition (Sun et al, 2021). Furthermore, the identified genes involved in carbon metabolism were highly expressed in the tolerant cultivar (Sun et al, 2021).…”

Section: Introductionmentioning

confidence: 98%

“…For example, low N stress reduced leaf activities of glutamine synthetase and glutamate synthetase in Kentucky bluegrass, but lower activity of nitrate reductase was found only in the sensitive cultivar exposed to low N condition (Sun et al, 2021). Furthermore, the identified genes involved in carbon metabolism were highly expressed in the tolerant cultivar (Sun et al, 2021). Low N stress can alter not only gene expression and protein synthesis but also the level of metabolites (Kusano et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…The supply of N below a certain level resulted in very poor color, shoot growth and yield of Kentucky bluegrass (Nelson, 1984). Moreover, low N stress decreased photosynthetic rate, chlorophyll content, chlorophyll fluorescence, and the nonphotochemical quenching, especially in the N-sensitive cultivar of Kentucky bluegrass (Sun et al, 2021). Reductions in plant height, shoot fresh and dry weight and shoot carbon content was also observed in the N-sensitive accession of perennial ryegrass (Lolium perenne L.) (Yao et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Differential Metabolomic Responses of Kentucky Bluegrass Cultivars to Low Nitrogen Stress

et al. 2022

Self Cite

View full text Add to dashboard Cite

Kentucky bluegrass (Poa pratensis L.) is a cool-season turfgrass species that responds strongly to nitrogen (N), but the metabolomic responses of this grass species to N supply is unknown. The N-tolerant cultivar Bluemoon and N-sensitive cultivar Balin were exposed to normal N (15 mM) and low N (0.5 mM) for 21 days for identification of differentially expressed metabolites (DEMs) between normal N and low N treatments. Balin had more reductions of chlorophyll and total soluble protein concentrations and a higher accumulation of superoxide radicals under low N stress. A total of 99 known DEMs were identified in either cultivar or both including 22 amino acids and derivatives, 16 carbohydrates, 29 organic acids, and 32 other metabolites. In Bluemoon, β-alanine metabolism was most enriched, followed by alanine, aspartate, and glutamate metabolism, biosynthesis of valine, leucine, and isoleucine biosynthesis, and glycine, serine, and threonine metabolism. In Balin, alanine, aspartate, and glutamate metabolism were most enriched, followed by the tricarboxylic acid (TCA), glyoxylate and decarbohydrate metabolism, and carbon fixation. Bluemoon generally maintained higher TCA cycle capacity and had more downregulated amino acids, while changes in more organic acids occurred in Balin under low N stress. Some metabolite changes by low-N stress were cultivar-specific. The results suggested that regulation of metabolites related to energy production or energy saving could contribute to low N tolerance in Kentucky bluegrass.

show abstract

Section: Plant Materials Growing Conditions and Experimental Designmentioning

confidence: 99%

Section: Plant Materials Growing Conditions and Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Differential Metabolomic Responses of Kentucky Bluegrass Cultivars to Low Nitrogen Stress

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

Effects of low nitrogen supply on nitrogen uptake, assimilation and remobilization in wild bermudagrass

Liu

Guo

et al. 2022

Plant Physiology and Biochemistry

View full text Add to dashboard Cite

Glutamine synthetase gene PpGS1.1 negatively regulates the powdery mildew resistance in Kentucky bluegrass

Sun

Xie

Chen

et al. 2022

Horticulture Research

Self Cite

View full text Add to dashboard Cite

Excessive nitrogen (N) application may induce powdery mildew (PM) in perennial grasses, but the resistance mechanisms to PM remain unclear. This study evaluated the physiological and molecular mechanisms of PM resistance affected by N supplies in Kentucky bluegrass (Poa pratensis L.). Cultivar ‘Bluemoon’ (N tolerant) and ‘Balin’ (N sensitive) were treated with low N (0.5 mM), normal N (15 mM), and high N (30 mM) for 21 d in a greenhouse. With increasing N levels, the disease growth was more severe in ‘Balin’ than in ‘Bluemoon’. RNA-seq and weighted gene coexpression network analysis revealed that the PpGS1.1 gene encoding glutamine synthetase was a potential hub gene for PM resistance after comparisons across cultivars and N treatments. The N metabolism pathway was connected with the plant-pathogen interaction pathway via PpGS1.1. The expression of PpGS1.1 in rice protoplasts indicated that the protein was located in the nucleus and cytoplasm. Overexpression of PpGS1.1 in wild type Kentucky bluegrass increased carbon and N contents, and the transgenic plants became more susceptible to PM with a lower wax density. The most differentially expressed genes (DEGs) for N metabolism were upregulated and DEGs for fatty acid metabolism pathway were downregulated in the overexpression lines. The results elucidated mechanisms of PM resistance in relation to N metabolism in Kentucky bluegrass.

show abstract

Nitrogen assimilation and gene regulation of two Kentucky bluegrass cultivars differing in response to nitrate supply

Cited by 8 publications

References 41 publications

Differential Metabolomic Responses of Kentucky Bluegrass Cultivars to Low Nitrogen Stress

Differential Metabolomic Responses of Kentucky Bluegrass Cultivars to Low Nitrogen Stress

Effects of low nitrogen supply on nitrogen uptake, assimilation and remobilization in wild bermudagrass

Glutamine synthetase gene PpGS1.1 negatively regulates the powdery mildew resistance in Kentucky bluegrass

Contact Info

Product

Resources

About