Nitrogen Catabolite Repression in members of Paracoccidioides complex

Cruz-Leite, Vanessa Rafaela Milhomem; Salem-Izacc, Sílvia Maria; Novaes, Evandro; Neves, Bruno J.; Brito, Wesley de Almeida; Silva, Lana O’Hara Souza; Paccez, Juliano D.; Parente-Rocha, Juliana Alves; Pereira, Maristela; Soares, Célia Maria de Almeida; Borges, Clayton Luiz

doi:10.1016/j.micpath.2020.104281

Cited by 9 publications

(9 citation statements)

References 109 publications

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“…RNA-seq analysis under SYA+N condition showed that MaNCP1 involved in multiple metabolic pathways, the amino acid permease MAC_09768, a DEG, is homologous to the amino acid permease GAP1 in S. cerevisiae, which is regulated by the Gln3 and Gat1 in S. cerevisiae [44]. It has been reported that the GATA transcription factor AreA, which function is similar to Gln3/Gat1, interacts with Gap1 in A. nidulans [37]. In this study, we confirmed that MaAreA was a direct target gene of MaNCP1.…”

Section: Discussionmentioning

confidence: 99%

“…Further analysis revealed that MAC_09768 (Figure 5D), a DEG involved in the RNA-seq data, encoding an amino acid permease, which was homologous to Saccharomyces cerevisiae amino acid permease GAP1 (NP_012965.3). GAP1 is an important marker protein to respond to NCR and a main regulator of yeast plasma membrane in nitrogen starvation condition [35,36], and could interact with AreA in A. nidulans [37]. Therefore, we reasoned that the involvement of MaNCP1 in regulating conidiation may be related to the NCR pathway.…”

Section: Transcriptomic Insights Into Pleiotropic Effects Of Mancp1mentioning

confidence: 99%

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The C2H2 Zinc Finger Protein MaNCP1 Contributes to Conidiation through Governing the Nitrate Assimilation Pathway in the Entomopathogenic Fungus Metarhizium acridum

Xia

Jin

2022

JoF

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Zinc finger proteins are an important class of multifunctional regulators. Here, the roles of a C2H2 zinc finger protein MaNCP1 (Metarhizium acridum nitrate-related conidiation pattern shift regulatory factor 1) in nitrogen utilization and conidiation were explored in the entomopathogenic fungus M. acridum. The results showed that MaNCP1-disruption mutant (ΔMaNCP1) impaired the ability to utilize nitrate, ammonium and glutamine and reduced the expression of nitrate assimilation-related genes, suggesting that MaNCP1 was involved in governing nitrogen utilization. In addition, the conidial yield of the ΔMaNCP1 strain, cultured on the microcycle conidiation medium (SYA), was significantly decreased, which could be restored or even enhanced than that of the WT strain through increasing the nitrate content in SYA medium. Further study showed that MaAreA, a core regulator in the nitrogen catabolism repression (NCR) pathway, was a downstream target gene of MaNCP1. Screening the differential expression genes between WT and ΔMaNCP1 strains revealed that the conidial yield of M. acridum regulated by nitrate might be related to NCR pathway on SYA medium. It could be concluded that MaNCP1 contributes to the nitrate assimilation and conidiation, which will provide further insights into the relationship between the nitrogen utilization and conidiation in fungi.

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Section: Discussionmentioning

confidence: 99%

Section: Transcriptomic Insights Into Pleiotropic Effects Of Mancp1mentioning

confidence: 99%

The C2H2 Zinc Finger Protein MaNCP1 Contributes to Conidiation through Governing the Nitrate Assimilation Pathway in the Entomopathogenic Fungus Metarhizium acridum

Xia

Jin

2022

JoF

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“…The yeast cells were then incubated in Brain Heart Infusion (BHI) medium supplemented with 4% (w/v) glucose at 36 • C under rotation for 48 h. The cells were centrifuged at 1900× g for 10 min at 4 • C and washed twice in phosphate-buffered saline (PBS). The yeast (2 × 10 6 cells/mL), was inoculated under NCR conditions, as described by Cruz-Leite et al [41]. In brief, the yeast cells were grown in Yeast Nitrogen Base (YNB) without amino acids and ammonium sulfate (Sigma-Aldrich ® , St. Louis, MO, USA), supplemented with either 10 mM glutamine or 10 mM proline nitrogen sources plus 2% glucose (w/v) for 48 h. Glutamine, a preferential nitrogen source, triggers repressive NCR conditions, while proline, a non-preferential nitrogen source, triggers alleviation of NCR [14].…”

Section: P Lutzii Growth Conditionsmentioning

confidence: 99%

“…The relative expression levels of the NCR genes formamidase (PAAG_03333), arginase (PAAG_01969), urease (PAAG_00954) and γ-glutamyl transpeptidase (PAAG_06130) were calculated using the standard curve method for relative quantification [43]. The tubulin gene (PAAG_03031) was used as an endogenous control, as described by Cruz-Leite et al [41]. Student's t-test was applied, and p ≤ 0.05 was considered as statistically significant.…”

Section: Rna Extraction and Quantitative Real-time Rt-qpcr Analysismentioning

confidence: 99%

“…NCR in Paracoccidioides spp. was first investigated by our group [41], demonstrating that NCR alleviation occurs in the presence of the non-preferential nitrogen source proline. To elucidate the global fungal metabolic pathway reprogramming in response to nitrogen availability, essential to infection establishment in host tissues, and explore NCR in P. lutzii, a proteomic approach was employed.…”

Section: Introductionmentioning

confidence: 99%

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Proteomics of Paracoccidioides lutzii: Overview of Changes Triggered by Nitrogen Catabolite Repression

Cruz-Leite,

Moreira,

Silva

et al. 2023

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Members of the Paracoccidioides complex are the causative agents of Paracoccidioidomycosis (PCM), a human systemic mycosis endemic in Latin America. Upon initial contact with the host, the pathogen needs to uptake micronutrients. Nitrogen is an essential source for biosynthetic pathways. Adaptation to nutritional stress is a key feature of fungi in host tissues. Fungi utilize nitrogen sources through Nitrogen Catabolite Repression (NCR). NCR ensures the scavenging, uptake and catabolism of alternative nitrogen sources, when preferential ones, such as glutamine or ammonium, are unavailable. The NanoUPLC-MSE proteomic approach was used to investigate the NCR response of Paracoccidioides lutzii after growth on proline or glutamine as a nitrogen source. A total of 338 differentially expressed proteins were identified. P. lutzii demonstrated that gluconeogenesis, β-oxidation, glyoxylate cycle, adhesin-like proteins, stress response and cell wall remodeling were triggered in NCR-proline conditions. In addition, within macrophages, yeast cells trained under NCR-proline conditions showed an increased ability to survive. In general, this study allows a comprehensive understanding of the NCR response employed by the fungus to overcome nutritional starvation, which in the human host is represented by nutritional immunity. In turn, the pathogen requires rapid adaptation to the changing microenvironment induced by macrophages to achieve successful infection.

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Research progress on the function and regulatory pathways of amino acid permeases in fungi

Yang,

Li,

Zhu

2024

World J Microbiol Biotechnol

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Nitrogen Catabolite Repression in members of Paracoccidioides complex

Cited by 9 publications

References 109 publications

The C2H2 Zinc Finger Protein MaNCP1 Contributes to Conidiation through Governing the Nitrate Assimilation Pathway in the Entomopathogenic Fungus Metarhizium acridum

The C2H2 Zinc Finger Protein MaNCP1 Contributes to Conidiation through Governing the Nitrate Assimilation Pathway in the Entomopathogenic Fungus Metarhizium acridum

Proteomics of Paracoccidioides lutzii: Overview of Changes Triggered by Nitrogen Catabolite Repression

Research progress on the function and regulatory pathways of amino acid permeases in fungi

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