Nitrogen Catabolite Repression in Yeasts and Filamentous Fungi

Jm, Wiame; Grenson, Marcelle; Hn, Arst

doi:10.1016/s0065-2911(08)60394-x

Cited by 301 publications

(295 citation statements)

References 230 publications

Supporting

Mentioning

281

Contrasting

Unclassified

Order By: Relevance

“…Additional energy and carbon are required by the cell to carry out the synthesis of its amino acids derived from both the carbon source and the ammonium sulfate in the media. The proton excess generated during amino acid synthesis must be pumped out of the cell to maintain the internal pH, resulting in acidification of the medium, which affects ammonium uptake and growth 42 .…”

Section: Discussionmentioning

confidence: 99%

“…Yeast are able to use a wide variety of compounds as a carbon and nitrogen source 6,41,42 . Brewer's wort is a typical example of a natural complex nutritional environment containing the sugars sucrose, fructose, glucose, maltose, maltotriose, dextrin material, as well as a complex mixture of amino acids, peptides, proteins, vitamins, ions, nucleic acids and other constituents 32 .…”

Section: Introductionmentioning

confidence: 99%

“…Nitrogen is one of the main elements found in many macromolecules of living organisms, playing a central role in structure and function, and most organisms have elaborate control mechanisms to provide a constant supply of nitrogen 6,18,19,34,42 . It has been observed that ammonia, asparagine, glutamine and glutamate are preferentially used by yeast 18,34 , which is attributed to a mechanism known as nitrogen catabolite repression 18 .…”

Section: Introductionmentioning

confidence: 99%

“…The utilisation of secondary nitrogen sources requires the synthesis of specific-catabolic enzymes and permeases, the expression of which is highly regulated by nitrogen catabolite repression. The latter is prevented in the presence of a preferred nitrogen source 18,42 . In wort, the assimilation of amino acids is ordered and groups of amino acids have been identified on the basis of their rate of removal from the fermentation broth 26 .…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effect of Sugar Catabolite Repression in Correlation with the Structural Complexity of the Nitrogen Source on Yeast Growth and Fermentation

Cruz

Batistote

Ernandes

2003

Journal of the Institute of Brewing

View full text Add to dashboard Cite

Biomass and ethanol production by industrial Saccharomyces cerevisiae strains were strongly affected by the structural complexity of the nitrogen source during fermentation in media containing galactose, and supplemented with a nitrogen source varying from a single ammonium salt (ammonium sulfate) to free amino acids (casamino acids) and peptides (peptone). Diauxie was observed at low galactose concentrations independent of nitrogen supplementation. At high sugar concentrations altered patterns of galactose utilisation were observed. Biomass accumulation and ethanol production depended on the nature of the nitrogen source and were different for baking and brewing ale and lager strains. Baking yeast showed improved galactose fermentation performance in the medium supplemented with casamino acids. High biomass production was observed with peptone and casamino acids for the ale brewing strain, however high ethanol production was observed only in the presence of casamino acids. Conversely, peptone was the nitrogen supplement that induced higher biomass and ethanol production for the lager brewing strain. Ammonium salts always induced poor yeast performance. The results with galactose differed from those obtained with glucose and maltose which indicated that supplementation with a nitrogen source in the peptide form (peptone) was more positive for yeast metabolism, suggesting that sugar catabolite repression has a central role in yeast performance in a medium containing nitrogen sources with differing levels of structural complexity.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Sugar Catabolite Repression in Correlation with the Structural Complexity of the Nitrogen Source on Yeast Growth and Fermentation

Cruz

Batistote

Ernandes

2003

Journal of the Institute of Brewing

View full text Add to dashboard Cite

show abstract

“…Further, when incremental amounts of glutamine are added to the PDA to invoke nitrogen-repressing conditions, the conidial pattern changes from a ring to a disk. In the absence of sufficient nitrogen, fungal cells can assimilate nitrates by reduction to ammonia, which is then converted to glutamate or glutamine; thus, primary nitrogen metabolism can occur when nitrates are the sole nitrogen source (Marzluf, 1997;Wiame et al, 1985). Hyphal cells store and translocate amino acids in a system which allows for the regulation of developmental genes by controlling the intracellular nitrogen status (Olsson, 1999;Watkinson, 1999).…”

Section: Carbon and Nitrogen Statusmentioning

confidence: 99%

Reproduction without sex: conidiation in the filamentous fungus Trichoderma

et al. 2010

View full text Add to dashboard Cite

2Lincoln Ventures Limited, PO Box 133, Lincoln University, Lincoln 7647, New Zealand Trichoderma spp. have served as models for asexual reproduction in filamentous fungi for over 50 years. Physical stimuli, such as light exposure and mechanical injury to the mycelium, trigger conidiation; however, conidiogenesis itself is a holistic response determined by the cell's metabolic state, as influenced by the environment and endogenous biological rhythms. Key environmental parameters are the carbon and nitrogen status and the C : N ratio, the ambient pH and the level of calcium ions. Recent advances in our understanding of the molecular biology of this fungus have revealed a conserved mechanism of environmental perception through the White Collar orthologues BLR-1 and BLR-2. Also implicated in the molecular regulation are the PacC pathways and the conidial regulator VELVET. Signal transduction cascades which link environmental signals to physiological outputs have also been revealed.

show abstract

TheS. cerevisiae nitrogen starvation-induced Yvh1p and Ptp2p phosphatases play a role in control of sporulation

Park

Beeser

Clancy

et al. 1996

Yeast

View full text Add to dashboard Cite

Starvation for nitrogen in the absence of a fermentable carbon source causes diploid Saccharomyces cerevisiae cells to leave vegetative growth, enter meiosis, and sporulate; the former nutritional condition also induces expression of the YVH1 gene that encodes a protein phosphatase. This correlation prompted us to determine whether the Yvh1p phosphatase was a participant in the network that controls the onset of meiosis and sporulation. We found that expression of the IME2 gene, encoding a protein kinase homologue required for meiosis‐ and sporulation‐specific gene expression, is decreased in a yvh1 disrupted strain. We also observed a decrease, albeit a smaller one, in the expression of IME1 which encodes an activator protein required for IME2 expression. Under identical experimental conditions, expression of the MCK1 and IME4 genes (which promote sporulation but do not require Ime1p for expression) was not affected. These results demonstrate the specificity of the yvh1 disruption phenotype. They suggest that decreased steady‐state levels of IME1 and IME2 mRNA were not merely the result of non‐specific adverse affects on nucleic acid metabolism caused by the yvh1 disruption. Sporulation of a homozygous yvh1 disruption mutant was delayed and less efficient overall compared to an isogenic wild‐type strain, a result which correlates with decreased IME1 and IME2 gene expression. We also observed that expression of the PTP2 tyrosine phosphatase gene (a negative regulator of the osmosensing MAP kinase cascade), but not the PTP1 gene (also encoding a tyrosine phosphatase) was induced by nitrogen‐starvation. Although disruption of PTP2 alone did not demonstrably affect sporulation or IME2 gene expression, sporulation was decreased more in a yvh1, ptp2 double mutant than in a yvh1 single mutant; it was nearly abolished in the double mutant. These data suggest that the YVH1 and PTP2 encoded phosphatases likely participate in the control network regulating meiosis and sporulation. Expression of YVH1 and PTP2 was not affected by nitrogen source quality (asparagine compared to proline) suggesting that nitrogen starvation‐induced YVH1 and PTP2 expression and sensitivity to nitrogen catabolite repression are on two different branches of the nitrogen regulatory network.

show abstract

Nitrogen Catabolite Repression in Yeasts and Filamentous Fungi

Cited by 301 publications

References 230 publications

Effect of Sugar Catabolite Repression in Correlation with the Structural Complexity of the Nitrogen Source on Yeast Growth and Fermentation

Effect of Sugar Catabolite Repression in Correlation with the Structural Complexity of the Nitrogen Source on Yeast Growth and Fermentation

Reproduction without sex: conidiation in the filamentous fungus Trichoderma

TheS. cerevisiae nitrogen starvation-induced Yvh1p and Ptp2p phosphatases play a role in control of sporulation

Contact Info

Product

Resources

About