The results also showed that wheat straw yield, plant height, spike length and tiller number have the same trend as those of wheat grain yield. Also, N, P, K and protein contents of wheat grains were increased by increasing N levels up to 120% of R. D. It is also clear that N contents of wheat grains were increased with timing of Cu applications in the order: Cu applied in two split doses at tillering + early boating stages> Cu applied at early boating stage ≥ Cu applied at tillering stage, while P and K contents haven't affected by Cu application time.In the same manner, grains N, P and K uptake followed the same trend as that of N, P, K and protein contents (%).