Nitrogen fertilization rate affects communities of ammonia-oxidizing archaea and bacteria in paddy soils across different climatic zones of China

Ren, Bingjie; Wang, Weiqi; Shen, Li‐dong; Yang, Wang-ting; Yang, Yumei; Jin, Jinghao; Geng, Caiyu

doi:10.1016/j.scitotenv.2023.166089

Cited by 3 publications

(1 citation statement)

References 74 publications

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“…On the one hand, long-term nitrogen application provided sufficient substrate for nitrifying microorganisms and increased microbial activity ( Wang et al, 2019 ), which in turn improved PNR. On the other hand, suppression of AOB reduced PNR probably owing to the fact that AOB is the major contributor to nitrification potential in neutral and alkaline soils ( Ren et al, 2023 ). Thus, inhibition of AOB by DMPP indirectly suppressed PNR.…”

Section: Discussionmentioning

confidence: 99%

The addition of discrimination inhibitors stimulations discrimination potential and N2O emissions were linked to predation among microorganisms in long term nitrogen application and straw returning systems

Jia,

Zhou,

et al. 2024

Front. Microbiol.

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IntroductionAmmonia oxidizing archaea (AOA) and ammonia oxidizing bacteria (AOB) have been proven to be key microorganisms driving the ammonia oxidation process. However, under different fertilization practices, there is a lack of research on the impact of interaction between predators and AOA or AOB on nitrogen cycling at the multi-trophic level.MethodsIn this study, a network-oriented microscopic culture experiment was established based on four different long-term fertilization practices soils. We used the nitrification inhibitors 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxide-3-oxyl (PTIO) and 3, 4-Dimethylpyrazole phosphate (DMPP) inhibited AOA and AOB, respectively, to explore the impact of interaction between protists and AOA or AOB on nitrogen transformation.ResultsThe results showed that long-term nitrogen application promoted the potential nitrification rate (PNR) and nitrous oxide (N2O) emission, and significantly increased the gene abundance of AOB, but had no obvious effect on AOA gene abundance. DMPP significantly reduced N2O emission and PNR, while PTIO had no obvious effect on them. Accordingly, in the multi-trophic microbial network, Cercozoa and Proteobacteria were identified as keystone taxa of protists and AOB, respectively, and were significantly positively correlated with N2O, PNR and nitrate nitrogen. However, Nitrososphaerota archaeon as the keystone species of AOA, had an obvious negative linkage to these indicators. The structural equation model (SEM) showed that AOA and AOB may be competitors to each other. Protists may promote AOB diversity through direct trophic interaction with AOA.ConclusionThe interaction pattern between protists and ammonia-oxidizing microorganisms significantly affects potential nitrification rate and N2O emission, which has important implications for soil nitrogen cycle.

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Section: Discussionmentioning

confidence: 99%

The addition of discrimination inhibitors stimulations discrimination potential and N2O emissions were linked to predation among microorganisms in long term nitrogen application and straw returning systems

Jia,

Zhou,

et al. 2024

Front. Microbiol.

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Nitrogen fertilization and soil nitrogen cycling: Unraveling the links among multiple environmental factors, functional genes, and transformation rates

Du,

Zhong,

Guo

et al. 2024

Science of The Total Environment

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Effects of a Vegetable Eel Co-Culture System on the Soil Ammonia-Oxidizing Microbial Community

Fan,

Bai,

et al. 2024

Agronomy

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(1) Background: A vegetable eel co-culture system is an economically efficient way of agricultural cultivation, which can have an impact on the soil microbial environment and play a pivotal role in the soil nutrient cycle, but there is little research on its impact on soil ammonia-oxidizing microorganisms. (2) Methods: NovaSeq platform sequencing was employed to investigate the richness, structure, and diversity of soil ammonia-oxidizing microbial communities, exploring the effects of a vegetable eel co-culture system on soil nitrogen cycling. Four different planting treatments were set up: unfertilized without vegetable eel (CCK), fertilized without vegetable eel (CRT), unfertilized with vegetable eel (ICK), and fertilized with vegetable eel (IRT). (3) Results: A vegetable eel co-culture system significantly increased soil pH and decreased bulk density under fertilization conditions. The soil nitrification potential rate was enhanced by a vegetable eel co-culture system to an average of 26.3%. A vegetable eel co-culture system significantly altered the community structure of all ammonia-oxidizing microorganisms, with a significant increase in the richness and diversity of ammonia-oxidizing bacteria (AOB) and comammox clade-A, while fertilization significantly increased the diversity of all ammonia-oxidizing microbial communities. Structural equation modeling (SEM) analysis showed that the main environmental factors affecting the structure of the ammonia-oxidizing microbial community were nitrate and total nitrogen. The number of amoA genes in AOB and comammox clade-B was significantly positively correlated with the soil potential N nitrification rate (PNR), which played a leading role in the nitrification of alkaline vegetable soil. The network analysis revealed that a vegetable eel co-culture system improved the modularity of AOB and comammox clade-B by 13.14% and 5.66%. (4) Conclusions: This study showed that the vegetable eel co-culture system stimulated the evolution of ammonia-oxidizing microbial communities by changing the physicochemical properties, which in turn promoted the soil nitrification reaction.

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Nitrogen fertilization rate affects communities of ammonia-oxidizing archaea and bacteria in paddy soils across different climatic zones of China

Cited by 3 publications

References 74 publications

The addition of discrimination inhibitors stimulations discrimination potential and N2O emissions were linked to predation among microorganisms in long term nitrogen application and straw returning systems

The addition of discrimination inhibitors stimulations discrimination potential and N2O emissions were linked to predation among microorganisms in long term nitrogen application and straw returning systems

Nitrogen fertilization and soil nitrogen cycling: Unraveling the links among multiple environmental factors, functional genes, and transformation rates

Effects of a Vegetable Eel Co-Culture System on the Soil Ammonia-Oxidizing Microbial Community

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