Nitrogen fixation in Pseudomonas stutzeri

Krotzky, Arno J.; Werner, Dietrich

doi:10.1007/bf00492904

Cited by 63 publications

(38 citation statements)

References 41 publications

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“…When sodium tungstate was added to the medium at between 0.1 and 5 mM, the proportion of ethane formed increased to 5%. This level was lowered to 0.1% when 10 Surprisingly, two previously accredited N2-fixing isolates of Pseudomonas stutzeri, CMT.9.A and JM300 (2,21), showed no homology to the nifDK probe (Fig. 3B).…”

Section: Methodsmentioning

confidence: 90%

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Detection of alternative nitrogenases in aerobic gram-negative nitrogen-fixing bacteria

1991

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Strains of aerobic, microaerobic, nonsymbiotic, and symbiotic dinitrogen-fixing bacteria were screened for the presence of alternative nitrogenase (N2ase) genes by DNA hybridization between genomic DNA and DNA encoding structural genes The biological reduction of dinitrogen (N2) to ammonia is catalyzed by a family of three nitrogenases (N2ases). One member of this family is the long-studied molybdenum enzyme (Mo-Fe N2ase), found in all diazotrophs. The other two alternative enzymes apparently do not require molybdenum and have been identified so far in only two obligately aerobic free-living bacteria in the genus Azotobacter. Azotobacter chroococcum can synthesize both a Mo-Fe N2ase and a vanadium nitrogenase (V-Fe N2ase) (32), while Azotobacter vinelandii makes these two enzymes (13, 14) plus a third enzyme type (Fe N2ase), which seems to require only Fe (8, 26).The enzymes are structurally similar. Each is composed of a pair of analogous 02-sensitive component proteins (8,11,13,14,32). Components 2 (Fe proteins) are homodimers which contain a single 4Fe-4S cluster, bind MgATP, and serve as highly specific electron donors to components 1, which probably contain the site of N2 binding and reduction. (17,28,34,35

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Section: Methodsmentioning

confidence: 90%

“…3B). Krotzky and Werner (21) suggested that nifgenes may be plasmid borne in this species, and hence it is possible that the strains have been cured for this plasmid. Alternatively, these organisms may possess a nitrogenase which is not closely genetically related to the enzymes from azotobacters.…”

Section: Methodsmentioning

confidence: 99%

Detection of alternative nitrogenases in aerobic gram-negative nitrogen-fixing bacteria

1991

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“…SELDIN and PENIDO (1986) have documented that when DNA from Bacillus azotofixans [Paenibacillus polymyxa] P3L-5 was treated with EcoRI, 2 fragments of 4.1 and 2.3 kb were hybridized with the 6.2 kb nif fragments on pSA30. A strain of Pseudomonas stutzeri isolated from the rhizosphere of a Sorghum nutans cultivar was identified as a nif-positive phenotype by DNA/DNA hybridization (KROTZKY and WERNER 1987). CHAN et al (1994) documented that some nif genes in a diazotrophic Pseudomonas sp.…”

Section: Discussionmentioning

confidence: 99%

Identification of nif genes in N₂‐fixing bacterial strains isolated from rice fields along the Yangtze River Plain

Xie

Cui

et al. 2006

J. Basic Microbiol.

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The aim of this research was to identify nifH and nifHDKYE ' genes in twenty strains of N2-fixing heterotrophic bacteria isolated from rice fields in the Yangtze River Plain. Southern hybridization of the total DNA from each strain was performed with the Klebsiella pneumoniae nifHDKYE ' gene probe (6.2 kb Eco RI fragment from pSA30) and the Azospirillum brasilense nifH gene probe (0.6 kb Eco RI-Hin dIII fragment from pHU8). We found that Eco RI fragments of total DNA from Aeromonas hydrophila HY2, Bacillus azotoformans FD, Bacillus licheniformis NCH1, NCH5, WH4, Bacillus brevis NC2, Bacillus pumilus NC12, Bacillus cereus NCH2, Citrobacter freundii HY5, HY9, Derxia gummosa HZ5, Pseudomonas mendocina HZ1 and Pseudomonas pseudoalcaligenes WH3 were positively hybridized with both of the probes. Agrobacterium radiobacter HY17, Corynebacterium sp. HY12, YZ and Pseudomonas sp. HY11 had Eco RI fragments hybridized with the K. pneumoniae nifHDKYE ' gene probe. An Eco RI fragment of total DNA from Bacillus megaterium YY4 was positively hybridized to the A. brasilense nifH gene probe. No hybridization sign was found in the total DNA fragments from Alcaligenes cupidus YY6 and Corynebacterium sp. NC11 hybridized with either of the gene probes. The data provide the number and size of EcoRI fragments of the total DNA hybridized with the nif gene probes for these strains of rarely studied species, suggesting additional evidence for N2 fixing and nif gene diversity of N2-fixing bacteria in rice fields along the Yangtze River Plain.

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“…When this plasmid is cured, there is an associated loss of N 2 fixation capability (189). No plasmid was detected in strain A1501.…”

Section: Nitrogen Fixationmentioning

confidence: 99%

Biology of Pseudomonas stutzeri

Lalucat

Bennasar

Bosch

et al. 2006

Microbiol Mol Biol Rev

552

418

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SUMMARY Pseudomonas stutzeri is a nonfluorescent denitrifying bacterium widely distributed in the environment, and it has also been isolated as an opportunistic pathogen from humans. Over the past 15 years, much progress has been made in elucidating the taxonomy of this diverse taxonomical group, demonstrating the clonality of its populations. The species has received much attention because of its particular metabolic properties: it has been proposed as a model organism for denitrification studies; many strains have natural transformation properties, making it relevant for study of the transfer of genes in the environment; several strains are able to fix dinitrogen; and others participate in the degradation of pollutants or interact with toxic metals. This review considers the history of the discovery, nomenclatural changes, and early studies, together with the relevant biological and ecological properties, of P. stutzeri.

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Nitrogen fixation in Pseudomonas stutzeri

Cited by 63 publications

References 41 publications

Detection of alternative nitrogenases in aerobic gram-negative nitrogen-fixing bacteria

Detection of alternative nitrogenases in aerobic gram-negative nitrogen-fixing bacteria

Identification of nif genes in N₂‐fixing bacterial strains isolated from rice fields along the Yangtze River Plain

Biology of Pseudomonas stutzeri

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Nitrogen fixation in Pseudomonas stutzeri

Cited by 63 publications

References 41 publications

Detection of alternative nitrogenases in aerobic gram-negative nitrogen-fixing bacteria

Detection of alternative nitrogenases in aerobic gram-negative nitrogen-fixing bacteria

Identification of nif genes in N2‐fixing bacterial strains isolated from rice fields along the Yangtze River Plain

Biology of Pseudomonas stutzeri

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Identification of nif genes in N₂‐fixing bacterial strains isolated from rice fields along the Yangtze River Plain