Nitrogen fixing cereal: A rising hero towards meeting food security

Priyadarshini, Parichita; Choudhury, Sharani; Tilgam, Jyotsana; Bharati, Alka; Sreeshma, N.

doi:10.1016/j.plaphy.2021.09.012

Cited by 18 publications

(1 citation statement)

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“…Bradyrhizobium is gram-negative bacteria, capable of symbiosis with leguminous plants (Avontuur et al, 2019). Bradyrhizobium induces the development of root nodules in leguminous plants and converts atmospheric di-nitrogen to ammonia using the nitrogenfixing enzyme called nitrogenase (Priyadarshini et al, 2021). In most cases, native nitrogen fixers can compete with the inoculum but there are no effective strains and it might not be compatible with the host plant (Irisarri et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Selection and evaluation of Bradyrhizobium inoculum for peanut, Arachis hypogea production in the Lao People’s Democratic Republic

Phimphong

Sibounnavong

Phommalath

et al. 2023

JANS

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The interaction between leguminous plants and Bradyrhizobium is limited, known as host specificity. Therefore, the selection of an appropriate Bradyrhizobia for use as biofertilizer inoculum for legumes is necessary. The Arachis hypogea L. is the most popular legume produced in the Lao People's Democratic Republic (PDR). Therefore, this research aimed to obtain the appropriate Bradyrhizobia that provides high efficiency in A. hypogea production in the Lao PDR. The 14 isolates were obtained from root nodules of A. hypogea L. trapped with Lao PDR soil samples. Three were the top isolates PMVTL-01, SMVTL-02, and BLXBL-03 showing high efficiency for peanut growth promotion. Strains PMVTL-01 and SMVTL-02 were closely related to the Bradyrhizobium geno sp. SA-3 Rp7b and B. zhanjiangense, respectively, whilst strain BLXBL-03 was closely related to Bradyrhizobium sp. CCBAU51745 and B. manausense BR3351. The competitiveness of these strains with Bradyrhizobium sp. SUTN9-2::GFP was analyzed, and only Bradyrhizobium sp. SMVTL-02 performed a number of occupied nodules higher than SUTN9-2::GFP. In addition, the competitiveness of the selected strain Bradyrhizobium sp. SMVTL-02 in a soil sample from the Lao PDR in the pot level was employed by tagging the SMVTL-02 with the DsRed gene. The results demonstrated that the DsRed-expressing tagged strain showed higher nodule occupancy than indigenous strains. Moreover, the results of the acetylene reduction assay (ARA), nodule number, nodule dry weight, and total plant dry weight from the pot experiment that inoculated with the SMVTL-02::DsRed were presented as having high potential to promote peanut growth as compared to non-inoculation. Thus, Bradyrhizobium sp. SMVTL-02 could be considered a potential biofertilizer inoculum for A. hypogea production in the Lao PDR.

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Section: Introductionmentioning

confidence: 99%