SUMMARYBased on a two-year field trial in the region of Campania (Southern Italy) the effects of water volume and nitrogen fertilization on the yield and quality of Burley tobacco (Nicotiana tabacum L.) were investigated with reference to the following traits: cured leaf yield, price index, yield value, leaf area, specific leaf weight, burning capacity, color parameters, total alkaloid, nitrate and chloride leaf content. The experimental design was a factorial comparison among three water volumes (40, 80 and 120% evapotranspiration (ET)), four nitrogen fertilization levels (0, 80, 160 and 240 kg ha -1 ) and two genotypes (cv TN86 and the hybrid R7-11). The yield of cured leaves rose with the increase in water and nitrogen availability, albeit at a decreasing rate. With the increase in water volume, the price index, burning capacity, specific leaf weight, total alkaloid and nitrate content decreased, while leaf area and chloride content increased. Up to a rate of 160 kg ha -1 , nitrogen fertilization increased the price index, yield value, burning capacity, leaf area, specific leaf weight, total alkaloid and nitrates, and reduced leaf chloride content especially at 40% ET water volume. Both, nitrogen fertilization and water volume had little influence on leaf color. The year had considerable effects on yield, leaf area and color parameters, with higher values in the rainier season. In the two years, genotype TN86 showed higher stability for yield and yield value, lower alkaloid and higher nitrate content in the leaf than the R7-11 hybrid.[Beitr. Tabakforsch. Int. 24 (2011) 194-206