Nitrogen isotopomer site preference of N2O produced by Nitrosomonas europaea and Methylococcus capsulatus Bath

Sutka, R. L.; Ostrom, Nathaniel E.; Ostrom, Peggy H.; Gandhi, Hasand; Breznak, John A.

doi:10.1002/rcm.968

Cited by 200 publications

(183 citation statements)

References 35 publications

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“…The ranges of d 15 N bulk -N 2 O from soil AOA were similar to those (-10 to -50%) from agricultural soils and forest soils (Kim and Craig, 1993;Rahn and Wahlen, 2000;Pérez et al, 2001;Rock et al, 2007). Previous studies found that the d 15 N bulk -N 2 O values of marine AOB (Nitrosomonas marina C-113) were about -10% with 20% O 2 concentration (Frame and Casciotti, 2010), whereas the d 15 N bulk -N 2 O values of N. europaea (ATCC 19718) in ammonia-oxidizing and nitrite-reducing conditions were -46.5% and -34.0%, respectively (Sutka et al, 2003(Sutka et al, , 2006. Surprisingly, the d 15 N bulk -N 2 O values of a marine AOA strain CN25 were positive (8.7%) (Santoro et al, 2011), which differed significantly from the ideal isotope fractionation effect and from those of the soil AOA strains.…”

Section: Aoa Strainsmentioning

confidence: 95%

“…. SP, the position-dependent isotope value of N 2 O, is an isotopic signature that is commonly used to interpret the source of N 2 O production (Toyoda and Yoshida, 1999;Sutka et al, 2003;Ostrom and Ostrom, 2011). Studies using pure AOB cultures have indicated that a positive SP (30-38%) is consistent with a N 2 O source from hydroxylamine oxidation (ammonia oxidation), whereas nitrifier denitrification (and denitrification) is thought to produce N 2 O with a SP value near or below zero (-10 to 0%) (Frame and Casciotti, 2010;Sutka et al, 2006).…”

Section: Aoa Strainsmentioning

confidence: 99%

“…The SP signatures of N 2 O can be used to calculate the magnitude of each pathway's contribution to the total N 2 O output based on its SP signature for AOB (Sutka et al, 2003;Sutka et al, 2006).…”

Section: N-labeling Experimentsmentioning

confidence: 99%

“…The contribution made by (Santoro et al, 2011). The black circles and triangles indicate N. europaea under hydroxylamine-oxidizing and denitrifying conditions, respectively, whereas the gray circle indicates N. europaea under ammonia-oxidizing conditions (Sutka et al, 2003;Sutka et al, 2006). The error bars are based on replicate experiments.…”

Section: N-labeling Experimentsmentioning

confidence: 99%

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Isotopic signatures of N2O produced by ammonia-oxidizing archaea from soils

Well²,

et al. 2013

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N 2 O gas is involved in global warming and ozone depletion. The major sources of N 2 O are soil microbial processes. Anthropogenic inputs into the nitrogen cycle have exacerbated these microbial processes, including nitrification. Ammonia-oxidizing archaea (AOA) are major members of the pool of soil ammonia-oxidizing microorganisms. This study investigated the isotopic signatures of N 2 O produced by soil AOA and associated N 2 O production processes. All five AOA strains (I.1a, I.1a-associated and I.1b clades of Thaumarchaeota) from soil produced N 2 O and their yields were comparable to those of ammonia-oxidizing bacteria (AOB). The levels of site preference (SP), d 15 N bulk and d 18 O -N 2 O of soil AOA strains were 13-30%, À 13 to À 35% and 22-36%, respectively, and strains MY1-3 and other soil AOA strains had distinct isotopic signatures. A 15 N-NH 4 þ -labeling experiment indicated that N 2 O originated from two different production pathways (that is, ammonia oxidation and nitrifier denitrification), which suggests that the isotopic signatures of N 2 O from AOA may be attributable to the relative contributions of these two processes. The highest N 2 O production yield and lowest site preference of acidophilic strain CS may be related to enhanced nitrifier denitrification for detoxifying nitrite. Previously, it was not possible to detect N 2 O from soil AOA because of similarities between its isotopic signatures and those from AOB. Given the predominance of AOA over AOB in most soils, a significant proportion of the total N 2 O emissions from soil nitrification may be attributable to AOA.

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Section: Aoa Strainsmentioning

confidence: 95%

Section: Aoa Strainsmentioning

confidence: 99%

Section: N-labeling Experimentsmentioning

confidence: 99%

Section: N-labeling Experimentsmentioning

confidence: 99%

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Isotopic signatures of N2O produced by ammonia-oxidizing archaea from soils

Well²,

et al. 2013

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“…SP, which is independent of the substrate's isotopic signature, has unique values reflecting microbial production pathways. SPs of N 2 O produced by NH 2 OH oxidation and by NO 2 -reduction were respectively about 33‰ and 0% [15][16][17][18] that can be a robust parameter in the analysis of mechanisms. In the last years, several efforts have been made to better understand the mechanisms of N 2 O production based on the isotopomer ratios of N 2 O in complex bacterial system during wastewater treatment [19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Source identification of N2O produced during simulated wastewater treatment under different oxygen conditions using stable isotopic analysis

et al. 2014

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Origin and fluxes of nitrous oxide along a latitudinal transect in western North Pacific: Controls and regional significance

Breider

Yoshikawa

Abe

et al. 2015

Global Biogeochemical Cycles

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Nitrous oxide (N 2 O) is an atmospheric trace gas playing an important role in both radiative forcing and stratospheric ozone depletion. The oceans are the second most important natural source of N 2 O. The magnitude of the flux of this source is poorly constrained. Moreover, the relative importance of the microbial processes leading to the formation or the consumption of N 2 O in oceans remains unclear. We present here fluxes and isotope and isotopomer signatures of N 2 O measured at three stations located along a latitudinal transect in subtropical and subarctic western North Pacific. These results indicate that about 30% to 55% of the oceanic flux of N 2 O to the atmosphere originates from the deep euphotic and shallow aphotic zones. The sea-to-air fluxes of N 2 O calculated using an isotope mass balance model indicate that the emission rate of N 2 O in subarctic waters is about 2 times higher than in oligotrophic subtropical waters suggesting that nutrient-rich water coming from the western subarctic gyre stimulates the N 2 O production. Moreover, isotopomer analysis has revealed that in shallow water N 2 O originates from nitrification and nitrifier denitrification processes, and its distribution in the water column is partly controlled by the incident solar radiation. The results of this study contribute to better constrain the global N 2 O budget and provide important information to better predict the future evolution of the oceanic emissions of N 2 O.

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Nitrogen isotopomer site preference of N₂O produced by Nitrosomonas europaea and Methylococcus capsulatus Bath

Cited by 200 publications

References 35 publications

Isotopic signatures of N2O produced by ammonia-oxidizing archaea from soils

Isotopic signatures of N2O produced by ammonia-oxidizing archaea from soils

Source identification of N2O produced during simulated wastewater treatment under different oxygen conditions using stable isotopic analysis

Origin and fluxes of nitrous oxide along a latitudinal transect in western North Pacific: Controls and regional significance

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