Nitrogen loss caused by denitrifying Nitrosomonas cells using ammonium or hydrogen as electron donors and nitrite as electron acceptor

Bock, Eberhard; Schmidt, Ingo; Stüven, Ralf; Zart, Dirk

doi:10.1007/bf00262198

Cited by 328 publications

(110 citation statements)

References 13 publications

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“…Contrary to suggestions of Conrad [22] and Bliecher-Mathiesen and Hoffman [23], oxidation of hydrogen sulfide with simultaneous reduction of nitrous oxide by thiobacilli [25] or hydrogen oxidation with concomitant nitrous oxide reduction described by Bock et al [26] show that nitrous oxide fate in the environment is more complex than can be inferred from our current state of knowledge of nitrogen cycle.…”

Section: Introductioncontrasting

confidence: 83%

Enrichment of nitrous oxide reducing bacteria from coastal marsh sediments

Nguyen¹,

Sobolev²

2013

OJE

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We attempted to recover organisms capable of respiratory nitrous oxide reduction with acetate as an electron donor from a variety of coastal marine sediments from Lavaca Bay area, Texas by use of liquid enrichment cultures. Putative positive cultures were analyzed by amplifying eubacterial and archaeal 16S rRNA gene fragments and analyzing their diversity by separating them by a denaturing gradient gel electrophoresis (DGGE). No Archaea was detected in our enrichments; however, positive enrichments from coastal salt marsh indicated the presence of putative nitrous oxide reducing bacteria. DGGE patterns of the amplified DNA were similar between enrichments, with ca. 7 obvious bands. The dominant bands were tentatively identified as members of the Gammaproteobacteria class, closely related to various denitrifying pseudomonads. Our results indicate that coastal marine environments may sustain a nitrous oxide reducing community, although nitrous oxide reduction is probably an opportunistic form of metabolism in that environment.

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Section: Introductioncontrasting

confidence: 83%

Enrichment of nitrous oxide reducing bacteria from coastal marsh sediments

Nguyen¹,

Sobolev²

2013

OJE

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“…Neither the expressed polypeptides of amoA and amoB nor the purified proteins from Nitrosomonas cell homogenates are able to oxidize ammonia to hydroxylamine (Hyman and Wood 1985;Hyman et al 1990). As shown by Bock et al (1995), the obligately lithoautotrophic Nitrosomonas eutropha is also a denitrifying organism that uses hydrogen as electron donor and nitrite as electron acceptor. Under anoxic and oxic conditions, N. eutropha is able to produce nitric oxide (NO) and nitrous oxide (N 2 O).…”

Section: Introductionmentioning

confidence: 98%

“…Moreover, in pure cultures of N. eutropha, nitrogen losses of up to 60% due to denitrification have been determined. N 2 has been detected as the main product (Bock et al 1995;Mulder et al 1995). It has been shown that obligately chemolithotrophic, ammoniaoxidizing bacteria of the genus Nitrosomonas are able to carry out nitrification and denitrification simultaneously under oxygen limitation.…”

Section: Introductionmentioning

confidence: 99%

Anaerobic ammonia oxidation with nitrogen dioxide by Nitrosomonas eutropha

Schmidt

Bock

1997

Archives of Microbiology

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228

175

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Nitrosomonas eutropha, an obligately lithoautotrophic bacterium, was able to nitrify and denitrify simultaneously under anoxic conditions when gaseous nitrogen dioxide (NO2) was supplemented to the atmosphere. In the presence of gaseous NO2, ammonia was oxidized, nitrite and nitric oxide (NO) were formed, and hydroxylamine occurred as an intermediate. Between 40 and 60% of the produced nitrite was denitrified to dinitrogen (N2). Nitrous oxide (N2O) was shown to be an intermediate of denitrification. Under an N2 atmosphere supplemented with 25 ppm NO2 and 300 ppm CO2, the amount of cell protein increased by 0.87 mg protein per mmol ammonia oxidized, and the cell number of N. eutropha increased by 5.8 x 10(9) cells per mmol ammonia oxidized. In addition, the ATP and NADH content increased by 4.3 μmol ATP (g protein)-1 and 6.3 μmol NADH (g protein)-1 and was about the same in both anaerobically and aerobically grown cells. Without NO2, the ATP content decreased by 0.7 μmol (g protein)-1, and the NADH content decreased by 1.2 μmol (g protein)-1. NO was shown to inhibit anaerobic ammonia oxidation.

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“…This suggests that the metabolic advantage could be conferred by a mutation of LasR. Although it seems unlikely that the mechanism is related to their potential role as a nitrogen source, both NO 3 Ϫ and NO 2 Ϫ might be utilized by strain SD-1 as electron acceptors (24).…”

Section: Discussionmentioning

confidence: 99%

“…In this context, the ability of P. aeruginosa to use NO 3 Ϫ and NO 2 Ϫ as part of electron transport is relevant (24). Either NO 3 Ϫ or NO 2 Ϫ can be used as an electron acceptor and in some conditions, particularly anaerobic ones, as a key metabolizing energy source (25), and quorum sensing has been reported to inhibit denitrification.…”

Section: Discussionmentioning

confidence: 99%

Nitrogen Source Stabilization of Quorum Sensing in the Pseudomonas aeruginosa Bioaugmentation Strain SD-1

Wang

Lai

Dandekar

et al. 2017

Appl Environ Microbiol

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Pseudomonas aeruginosa SD-1 is efficient at degrading aromatic compounds and can therefore contribute to the bioremediation of wastewater. P. aeruginosa uses quorum sensing (QS) to regulate the production of numerous secreted "public goods." In wastewater bioaugmentation applications, there are myriad nitrogen sources, and we queried whether various nitrogen sources impact the stabilities of both QS and the bacterial populations. In a laboratory strain of P. aeruginosa, PAO1, the absence of a nitrogen source has been shown to destabilize these populations through the emergence of QS mutant "cheaters." We tested the ability of SD-1 to grow in casein broth, a condition that requires QS for growth, when the nitrogen source with either NH 4 Cl, NaNO 3 , or NaNO 2 or with no added nitrogen source. There was great variability in susceptibility to invasion by QS mutant cheaters and, by extension, the stability of the SD-1 population. When grown with NH 4 Cl as an extra nitrogen source, no population collapse was observed; by contrast, twothirds of cultures grown in the presence of NaNO 2 collapsed. In the populations that collapsed, the frequency of social cheaters exceeded 40%. NaNO 3 and NaNO 2 directly favor QS mutants of P. aeruginosa SD-1. Although the mechanism by which these nitrogen sources act is not clear, these data indicate that the metabolism of nitrogen can affect the stability of bacterial populations, an important observation for continuing industrial applications with this species.IMPORTANCE Bioaugmentation as a method to help remediate wastewater pollutant streams holds significant potential to enhance traditional methods of treatment. Addition of microbes that can catabolize organic pollutants can be an effective method to remove several toxic compounds. Such bioaugmented strains of bacteria have been shown to be susceptible to competition from the microbiota that are present in wastewater streams, limiting their potential effectiveness. Here, we show that bioaugmentation strains of bacteria might also be susceptible to invasion by social cheaters and that the nitrogen sources available in the wastewater might influence the ability of cheaters to overtake the bioaugmentation strains. Our results imply that control over the nitrogen sources in a wastewater stream or selective addition of certain nitrogen sources could help stabilize bioaugmentation strains of bacteria.KEYWORDS quorum sensing, social cheating, bioaugmentation, long-term stabilization, inorganic nitrogen B ioaugmentation, the use of selected microbial strains in activated sludge to strengthen their abilities for pollutant removal (1), has significant advantages in the degradation of toxic and hazardous compounds. These advantages include cost and

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Nitrogen loss caused by denitrifying Nitrosomonas cells using ammonium or hydrogen as electron donors and nitrite as electron acceptor

Cited by 328 publications

References 13 publications

Enrichment of nitrous oxide reducing bacteria from coastal marsh sediments

Enrichment of nitrous oxide reducing bacteria from coastal marsh sediments

Anaerobic ammonia oxidation with nitrogen dioxide by Nitrosomonas eutropha

Nitrogen Source Stabilization of Quorum Sensing in the Pseudomonas aeruginosa Bioaugmentation Strain SD-1

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