Nitrogen oxide cycle regulates nitric oxide levels and bacterial cell signaling

Sasaki, Yasuyuki; Oguchi, Haruka; Kobayashi, Takuya; Kusama, Shinichiro; Sugiura, Ryo; Moriya, Kenta; Hirata, Tatsumi; Yukioka, Yuriya; Takaya, Naoki; Yajima, Seishi; Ito, Shinsaku; Okada, Kiyoshi; Ohsawa, Kanju; Ikeda, Haruo; Takano, Hideaki; Uéda, Kenji; Shoun, Hirofumi

doi:10.1038/srep22038

Cited by 48 publications

(45 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: New Perspectives In Growth and Development Of Streptomycesmentioning

confidence: 92%

“…However, endogenous nitric oxide production involving conserved actinobacterial genes has now been convincingly demonstrated in S. coelicolor 84 and mycobacteria 85, 86 ( Figure 3). Thus, in S. coelicolor , organic nitrogen is converted by an unknown route to nitrate, which is reduced by nitrate reductase to nitrite 84 . This mainly involves NarG2, one of three nitrate reductases in S. coelicolor : NarG2 is predominant in growing hyphae and is induced by moderate hypoxic downshift, while NarG3 has a subordinate role and NarG1 is mainly active during germination 87 .…”

Section: New Perspectives In Growth and Development Of Streptomycesmentioning

confidence: 92%

“…This mainly involves NarG2, one of three nitrate reductases in S. coelicolor : NarG2 is predominant in growing hyphae and is induced by moderate hypoxic downshift, while NarG3 has a subordinate role and NarG1 is mainly active during germination 87 . Nitrite was shown in turn to be the source of nitric oxide (albeit again by an unknown route) 84 . This source of nitric oxide mirrors a pathway important in eukaryotes 88 , where endogenous nitric oxide is a major physiological signalling molecule, even influencing epigenetic changes 89 .…”

Section: New Perspectives In Growth and Development Of Streptomycesmentioning

confidence: 99%

“…In the nitrogen oxide cycle 84 , nitrate generated intracellularly by an unknown pathway is reduced to nitrite mainly by the NarG2 enzyme 87 . Nitrite is the source of nitric oxide (NO) (mechanism unknown) 84 .…”

Section: New Perspectives In Growth and Development Of Streptomycesmentioning

confidence: 99%

“…Nitrite is the source of nitric oxide (NO) (mechanism unknown) 84 . Nitric oxide is oxidised to nitrate by flavohaemoglobins specified by SCO7428 (incorrectly given as SCO7472 in 84) and SCO7094, which are induced when nitric oxide binds to NsrR, the repressor of both genes 84, 90 . Internal nitric oxide also binds the haem-containing sensor kinase DevS, which then phosphorylates the cognate response regulator DevR.…”

Section: New Perspectives In Growth and Development Of Streptomycesmentioning

confidence: 99%

See 4 more Smart Citations

Recent advances in understanding Streptomyces

2016

View full text Add to dashboard Cite

About 2,500 papers dated 2014–2016 were recovered by searching the PubMed database for Streptomyces, which are the richest known source of antibiotics. This review integrates around 100 of these papers in sections dealing with evolution, ecology, pathogenicity, growth and development, stress responses and secondary metabolism, gene expression, and technical advances. Genomic approaches have greatly accelerated progress. For example, it has been definitively shown that interspecies recombination of conserved genes has occurred during evolution, in addition to exchanges of some of the tens of thousands of non-conserved accessory genes. The closeness of the association of Streptomyces with plants, fungi, and insects has become clear and is reflected in the importance of regulators of cellulose and chitin utilisation in overall Streptomyces biology. Interestingly, endogenous cellulose-like glycans are also proving important in hyphal growth and in the clumping that affects industrial fermentations. Nucleotide secondary messengers, including cyclic di-GMP, have been shown to provide key input into developmental processes such as germination and reproductive growth, while late morphological changes during sporulation involve control by phosphorylation. The discovery that nitric oxide is produced endogenously puts a new face on speculative models in which regulatory Wbl proteins (peculiar to actinobacteria) respond to nitric oxide produced in stressful physiological transitions. Some dramatic insights have come from a new model system for Streptomyces developmental biology, Streptomyces venezuelae, including molecular evidence of very close interplay in each of two pairs of regulatory proteins. An extra dimension has been added to the many complexities of the regulation of secondary metabolism by findings of regulatory crosstalk within and between pathways, and even between species, mediated by end products. Among many outcomes from the application of chromosome immunoprecipitation sequencing (ChIP-seq) analysis and other methods based on “next-generation sequencing” has been the finding that 21% of Streptomyces mRNA species lack leader sequences and conventional ribosome binding sites. Further technical advances now emerging should lead to continued acceleration of knowledge, and more effective exploitation, of these astonishing and critically important organisms.

show abstract

Section: New Perspectives In Growth and Development Of Streptomycesmentioning

confidence: 92%

Section: New Perspectives In Growth and Development Of Streptomycesmentioning

confidence: 92%

Section: New Perspectives In Growth and Development Of Streptomycesmentioning

confidence: 99%

Section: New Perspectives In Growth and Development Of Streptomycesmentioning

confidence: 99%

Section: New Perspectives In Growth and Development Of Streptomycesmentioning

confidence: 99%

See 3 more Smart Citations

Recent advances in understanding Streptomyces

2016

View full text Add to dashboard Cite

show abstract

Recent Advances in Soft Materials to Build and Functionalize Hard Structures for Electrochemical Energy Storage and In situ Electrochemical Molecular Biosensing

Guo

2016

Adv Funct Materials

View full text Add to dashboard Cite

Soft materials such as carbon materials, polymers and macromolecules possess rich functional groups and have become an important research filed in material science and engineering but they lack high durability and mechanical rigidity. Hard structures can provide stable and rigid scaffolds as well as hierarchically porous structures for superior physical properties; however, they often suffer from relatively poor surface chemistry. Construction of soft materials functionalized hard structures often generates new composite materials with unique physico‐chemical properties for synergistically boosting high‐efficient energy conversion/storage and biosensing. In this review, starting with introduction of fundamentals and properties of soft materials and hard structures, advances in fabrications and characterizations of the composite materials are discussed. The excellent physico‐chemical and electrochemical properties of the soft materials‐built and functionalized composite materials as well as their applications as electrode materials in solar cells, fuel cells, electrochemical water splitting, lithium‐ion batteries/lithium‐sulfur batteries/supercapacitors, and in situ molecular biosensing for greatly improved performance are surveyed. The performance enhancement mechanisms in these electrochemical systems are discussed to elicit fundamental insights. The challenges and prospects of this research area are also deliberated to inspire future improvements.

show abstract

Machine‐Learning Analysis of Streptomyces coelicolor Transcriptomes Reveals a Transcription Regulatory Network Encompassing Biosynthetic Gene Clusters

Lee,

Choe,

Palsson

et al. 2024

Advanced Science

View full text Add to dashboard Cite

Streptomyces produces diverse secondary metabolites of biopharmaceutical importance, yet the rate of biosynthesis of these metabolites is often hampered by complex transcriptional regulation. Therefore, a fundamental understanding of transcriptional regulation in Streptomyces is key to fully harness its genetic potential. Here, independent component analysis (ICA) of 454 high‐quality gene expression profiles of the model species Streptomyces coelicolor is performed, of which 249 profiles are newly generated for S. coelicolor cultivated on 20 different carbon sources and 64 engineered strains with overexpressed sigma factors. ICA of the transcriptome dataset reveals 117 independently modulated groups of genes (iModulons), which account for 81.6% of the variance in the dataset. The genes in each iModulon are involved in specific cellular responses, which are often transcriptionally controlled by specific regulators. Also, iModulons accurately predict 25 secondary metabolite biosynthetic gene clusters encoded in the genome. This systemic analysis leads to reveal the functions of previously uncharacterized genes, putative regulons for 40 transcriptional regulators, including 30 sigma factors, and regulation of secondary metabolism via phosphate‐ and iron‐dependent mechanisms in S. coelicolor. ICA of large transcriptomic datasets thus enlightens a new and fundamental understanding of transcriptional regulation of secondary metabolite synthesis along with interconnected metabolic processes in Streptomyces.

show abstract

Nitrogen oxide cycle regulates nitric oxide levels and bacterial cell signaling

Cited by 48 publications

References 41 publications

Recent advances in understanding Streptomyces

Recent advances in understanding Streptomyces

Recent Advances in Soft Materials to Build and Functionalize Hard Structures for Electrochemical Energy Storage and In situ Electrochemical Molecular Biosensing

Machine‐Learning Analysis of Streptomyces coelicolor Transcriptomes Reveals a Transcription Regulatory Network Encompassing Biosynthetic Gene Clusters

Contact Info

Product

Resources

About