Nitrogen removal, microbial community and electron transport in an integrated nitrification and denitrification system for ammonium-rich wastewater treatment

Zeng, Danfei; Jia, Miao; Wu, Guangxue; Zhan, Xinmin

doi:10.1016/j.ibiod.2018.07.014

Cited by 68 publications

(13 citation statements)

References 47 publications

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“…AMO, NAR, and NIR obtain electrons from ETS, and their ability to do this is directly related to nitrogen removal efficiency. 45 Hence, the presence of PS resulted in the decreased enzyme activity, which is an important cause of reduced ETSA. 46,47 In our study, PS adhered to the surface of cell membranes, changing the membrane structure (Figure 3), which may also influence the low ETSA in microbial membrane.…”

Section: Nanoplastics Disrupt Microbialmentioning

confidence: 99%

Nanoplastics Disturb Nitrogen Removal in Constructed Wetlands: Responses of Microbes and Macrophytes

Yang

Guo

et al. 2020

Environ. Sci. Technol.

193

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Nanosized plastics (nanoplastics) releasing into the wastewater may pose a potential threat to biological nitrogen removal. Constructed wetland (CW), a wastewater treatment or shore buffer system, is an important sink of nanoplastics, while it is unclear how nitrogen removal in CWs occurs in response to nanoplastics. Here, we investigated the effects of polystyrene (PS) nanoplastics (0, 10, and 1000 μg/L) on nitrogen removal for 180 days in CWs. The results revealed that total nitrogen removal efficiency decreased by 29.5–40.6%. We found that PS penetrated the cell membrane and destroyed both membrane integrity and reactive oxygen species balance. Furthermore, PS inhibited microbial activity in vivo, including enzyme (ammonia monooxygenase, nitrate reductase, and nitrite reductase) activities and electron transport system activity (ETSA). These adverse effects, accompanied by a decline in the relative abundance of nitrifiers (e.g., Nitrosomonas and Nitrospira) and denitrifiers (e.g., Thauera and Zoogloea), directly accounted for the strong deterioration observed in nitrogen removal. The decline in leaf and root activities decreased nitrogen uptake by plants, which is an important factor of deterioration in nitrogen removal. Overall, our results imply that the presence of nanoplastics in the aquatic environment is a hidden danger to the global nitrogen cycle and should receive more attention.

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Section: Nanoplastics Disrupt Microbialmentioning

confidence: 99%

Nanoplastics Disturb Nitrogen Removal in Constructed Wetlands: Responses of Microbes and Macrophytes

Yang

Guo

et al. 2020

Environ. Sci. Technol.

193

View full text Add to dashboard Cite

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“…In the case of the research conducted by Wang et al [47], the nitrification process took place in industrial wastewater, where the concentration of ammonium nitrogen was 450.00 mg•dm −3 . On the other hand, Zeng et al [48] observed nitrification at concentrations of NH4-N ranging from 300.00-900.00 mg•dm −3 . The results obtained in the research carried out at the Municipal and Industrial Sewage Treatment Plant in Oświęcim did not confirm these observations.…”

Section: Discussionmentioning

confidence: 97%

Toxic Effect of Ammonium Nitrogen on the Nitrification Process and Acclimatisation of Nitrifying Bacteria to High Concentrations of NH4-N in Wastewater

et al. 2021

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The aim of the conducted research was to assess the effectiveness of the nitrification process, at different concentrations of ammonium nitrogen, in biologically treated wastewater in one of the largest municipal and industrial wastewater treatment plants in Poland. The studies also attempted to acclimate nitrifying bacteria to the limited concentration of ammonium nitrogen and determined the efficiency of nitrification under the influence of acclimated activated sludge in the biological wastewater treatment system. The obtained results indicate that the concentration of ammonium nitrogen above 60.00 mg·dm−3 inhibits nitrification, even after increasing the biomass of nitrifiers. The increase in the efficiency of the nitrification process in the tested system can be obtained by using the activated sludge inoculated with nitrifiers. For this purpose, nitrifiers should be preacclimated, at least for a period of time, allowing them to colonize the activated sludge. The acclimated activated sludge allows reducing the amount of ammonium nitrogen in treated sewage by approx. 35.0%. The process of stable nitrification in the biological treatment system was observed nine days after introducing the acclimated activated sludge into the aeration chamber.

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“…With the increase of Fe(II)EDTA–NO concentration, the relative abundance of Thauera and Dokdonella decreased from 43.2 to 3.1% (R1 to R3) and 6.0% to nearly 0% (R1 to R4), respectively. Thauera, the most abundant genus in the control sample from initial sludge (R1), is one of the β-Proteobacteria genera and capable of conducting complete denitrification. − It has been proven that Thauera had more N 2 OR-encoding gene, accounting for (8.4–35.7%) of the total quantity, than other genera in denitrifying systems. , Dokdonella is a γ-Proteobacteria genus and has been categorized as nitrogen-removing organisms. , Previously, Wang et al found that the abundance of Dokdonella was positively correlated with gene nosZ , which could encode N 2 OR, via correlation analysis of functional bacteria and genes in a denitrification system. However, no direct evidence for NO reduction-related mechanism of Thauera and Dokdonella has been elucidated, indicating these two genera might have no extra efficient resistance toward a large amount of Fe(II)EDTA–NO, and thus, their relative abundance decreased as NO increased.…”

Section: Discussionmentioning

confidence: 99%

Biological Reduction of Nitric Oxide for Efficient Recovery of Nitrous Oxide as an Energy Source

Wang

Chen

Wei

et al. 2021

Environ. Sci. Technol.

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Chemical absorption–biological reduction based on Fe(II)EDTA is a promising technology to remove nitric oxide (NO) from flue gases. However, limited effort has been made to enable direct energy recovery from NO through production of nitrous oxide (N2O) as a potential renewable energy rather than greenhouse gas. In this work, the enhanced energy recovery in the form of N2O via biological NO reduction was investigated by conducting short-term and long-term experiments at different Fe(II)EDTA–NO and organic carbon levels. The results showed both NO reductase and N2O reductase were inhibited at Fe(II)EDTA–NO concentration up to 20 mM, with the latter being inhibited more significantly, thus facilitating N2O accumulation. Furthermore, N2O accumulation was enhanced under carbon-limiting conditions because of electron competition during short-term experiments. Up to 47.5% of NO–N could be converted to gaseous N2O–N, representing efficient N2O recovery. Fe(II)EDTA–NO reduced microbial diversity and altered the community structure toward Fe(II)EDTA–NO-reducing bacteria-dominated culture during long-term experiments. The most abundant bacterial genus Pseudomonas, which was able to resist the toxicity of Fe(II)EDTA–NO, was significantly enriched, with its relative abundance increased from 1.0 to 70.3%, suggesting Pseudomonas could be the typical microbe for the energy recovery technology in NO-based denitrification.

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Nitrogen removal, microbial community and electron transport in an integrated nitrification and denitrification system for ammonium-rich wastewater treatment

Cited by 68 publications

References 47 publications

Nanoplastics Disturb Nitrogen Removal in Constructed Wetlands: Responses of Microbes and Macrophytes

Nanoplastics Disturb Nitrogen Removal in Constructed Wetlands: Responses of Microbes and Macrophytes

Toxic Effect of Ammonium Nitrogen on the Nitrification Process and Acclimatisation of Nitrifying Bacteria to High Concentrations of NH4-N in Wastewater

Biological Reduction of Nitric Oxide for Efficient Recovery of Nitrous Oxide as an Energy Source

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