Nitrogen uptake by the macro-algae Cladophora coelothrix and Cladophora parriaudii: Influence on growth, nitrogen preference and biochemical composition

Ross, Michael E.; Davis, Katharine Anne; McColl, R. H. S.; Stanley, Michele S.; Day, John; Semiao, Andrea

doi:10.1016/j.algal.2017.12.005

Cited by 86 publications

(41 citation statements)

References 49 publications

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“…On the other hand, macroalgae release both organic nitrogen 53 and inorganic nitrogen reserves 31 during active growth and so have the potential to assimilate nutrients in the environment upon translocation. Experimental macroalgae cultured with ammonium may use this internal reserve for growth instead of assimilating nitrate in the water column, 16 and may therefore not integrate the local δ 15 N signature completely.…”

Section: Discussionmentioning

confidence: 99%

“…Reduction occurs through reducing enzymes present in macroalgae, such as nitrate and nitrite reductase 16,26 . These nitrate‐reducing enzymes can be temporarily switched off as a result of ammonium assimilation; therefore, ammonium assimilation can inhibit nitrate being simultaneously assimilated in macroalgae 16,26 . This is particularly relevant to this study, whereby transplanted macroalgae could preferentially assimilate the δ 15 N signal of ammonium present in an estuary rather than that of the nitrate present, leading to inaccurate interpretations of the nitrogen sources present 11 …”

Section: Macroalgal Physiologymentioning

confidence: 99%

“…In the natural environment, ammonium and nitrate ions are considered the most common nitrogen sources, and as such are frequently used in laboratory experiments 26 . Macroalgae have been shown to preferentially uptake ammonium over nitrate 14,16 . Alwyn and Rees 14 found that K m (the concentration of a nutrient that gives half of the maximum rate of uptake) and V max (the maximum rate of uptake achieved at saturating conditions of the nutrient) are greater for ammonium uptake than for nitrate uptake for a range of macroalgal species.…”

Section: Macroalgal Physiologymentioning

confidence: 99%

“…Fractionation processes in the water column, or during nitrogen uptake, could mean that this assumption is invalid 12,13 . Nitrogen uptake is often determined by many different environmental factors such as light, temperature, nitrogen species and morphological factors 14–16 . Despite these issues, δ 15 N values in macroalgae are generally believed to be representative of biologically available nitrogen in estuaries 15 .…”

Section: Introductionmentioning

confidence: 99%

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Isotopically labelled macroalgae: A new method for determining sources of excess nitrogen pollution

Bailes

Gröcke

2020

Rapid Comm Mass Spectrometry

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Rationale Stable nitrogen isotope ratios (δ15N) can be used to discern sources of excess nitrogen pollution in water. The δ15N values of nitrate in water often do not reflect the true δ15N source value owing to high temporal variation, and there are high analytical costs associated with obtaining δ15N values from water nitrate. To find alternative solutions, we isotopically labelled macroalgae (i.e. seaweed) beyond natural variation as a new method for determining sources of excess nitrogen pollution in seawater. Methods Fucus vesiculosus (bladder wrack) non‐fertile tips were collected from Easington Colliery, County Durham, UK, and cultured in two isotopically enriched solutions containing ammonium sulphate with δ15N values of 170 ± 5‰ and –60 ± 3‰ for a period of 19 days. The macroalgae were cultured in separate opened glass jars in an incubator with set temperature (11°C) and light (125 μmol photons m−2 s−2 on a light/dark rhythm of 16 h/8 h). The oven‐dried tips were analysed for δ15N over the 19‐day experiment. Results The macroalgal tips incorporated the isotopically enriched solutions rapidly, reaching 50% of the isotopically enriched seawater after ca 11 days for the 15N‐enriched solution and ca 15 days for the 14N‐enriched solution. δ15N values were incorporated more into the torn base of the macroalgal tips than into the middle and apex regions. Conclusions F. vesiculosus rapidly incorporates the isotopic ratio of the artificial seawater solution to which it is translocated. The laboratory‐developed isotopically labelled macroalgae can be manufactured to generate ‘unnatural’ δ15N values for translocation into coastal environments. This approach can provide an efficient, low‐cost alternative to current analytical methods for determining and monitoring nitrogen pollution.

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Section: Discussionmentioning

confidence: 99%

Section: Macroalgal Physiologymentioning

confidence: 99%

Section: Macroalgal Physiologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isotopically labelled macroalgae: A new method for determining sources of excess nitrogen pollution

Bailes

Gröcke

2020

Rapid Comm Mass Spectrometry

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“…Nevertheless, filamentous algae like Cladophora sp. can be harvested and used as a secondary product for compost, biofertilizer, bioestimulants or as a good source of cellulose fibers, helping farmers obtain alternative resources (Wilkie and Mulbry, 2002;Ross et al, 2018;Sucaldito and Camacho 2017).…”

mentioning

confidence: 99%

Removal of nutrients from Organic Liquid Agricultural Waste using filamentous algae.

et al. 2021

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A feasibility analysis of tertiary treatment for Organic Liquid Agricultural Waste is presented using filamentous algae belonging to the genus Cladophora sp. as an alternative to chemical tertiary treatment. The main advantages of tertiary treatments that use biological systems are the low cost investment and the minimal dependence on environmental variables. In this work we demonstrate that filamentous algae reduces the nutrient load of nitrate (circa 75%) and phosphate (circa 86%) from the organic waste effluents coming from dairy farms after nine days of culture, with the added advantage being that after the treatment period, algae removal can be achieved by simple procedures. Currently, the organic wastewater is discarded into fields and local streams. However, the algae can acquire value as a by-product since it has various uses as compost, cellulose, and biogas. A disadvantage of this system is that clean water must be used to achieve enough water transparency to allow algae growth. Even so, the nutrient reduction system of the organic effluents proposed is friendly to the ecosystem, compared to tertiary treatments that use chemicals to precipitate and collect nutrients such as nitrates and phosphates.

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Monitoring microalgal growth of Chlorella minutissima with a new all solid‐state contact nitrate selective sensor

Balkanli

Işıldak

İnan

et al. 2022

Biotechnology Progress

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As third generation feedstock, microalgae are microorganisms that can grow only in the optimum conditions. There are parameters including the concentration of macro and microelements in nutrient solution, pH, temperature and light intensity that have significant impact on microalgal growth. In recent years, various sensing devices have been developed for sensitive measurement of these parameters during microalgal growth. In this study, a new potentiometric nitrate selective sensor was developed to indicate the nitrate uptake of microalgae and the effect of nitrate nutrient on microalgal growth, specifically, and this sensor was successfully applied to determine nitrate concentration in medium during microalgal growth. Moreover, the effects of nitrate, carbonate and phosphate concentration in the growth medium on biomass production of Chlorella minutissima were investigated by using Box–Behnken design method, and optimum conditions were determined for the highest biomass production of microalgae. As a result of the experiments, it was seen that the highest C. minutissima production was achieved using the medium consist of 2.63 g/L NaNO3, 0.35 g/L Na2CO3 and 0.4 g/L KH2PO4. Statistically, it was observed that there was a proportional relationship between the microalgae production and investigated parameters such as carbon, nitrogen and phosphate amounts of culture mediums. The electrode showed a wide linear range between 1.0 × 10−1 and 5.0 × 10−5 M with a detection limit of the 5 × 10−6 M and the response time was found as 10 s. The results showed that developed nitrate selective sensor could be successfully applied for continuous measurement of nitrate in microalgal productions at reduced cost.

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Nitrogen uptake by the macro-algae Cladophora coelothrix and Cladophora parriaudii: Influence on growth, nitrogen preference and biochemical composition

Cited by 86 publications

References 49 publications

Isotopically labelled macroalgae: A new method for determining sources of excess nitrogen pollution

Isotopically labelled macroalgae: A new method for determining sources of excess nitrogen pollution

Removal of nutrients from Organic Liquid Agricultural Waste using filamentous algae.

Monitoring microalgal growth of Chlorella minutissima with a new all solid‐state contact nitrate selective sensor

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