A small-plot experiment was made to assess the influence on dry-matter output from grass of a wide range of fertilizer nitrogen and defoliation interval treatments. There were five defoliation treatments, 22, 28, 45, 75 and 112-day regrowth intervals throughout the growing season each at six levels of nitrogen application, ranging by 300 kg increments from 0 to 1500 kg/ha/year.There was a marked interaction effect between treatments; a positive dry-matter response was maintained to a higher level of applied nitrogen with more frequent defoliation. In 2 years out of 3 maximum dry-matter yield was produced under a 75-day defoliation interval although the mean yield advantage over a 45-day defoliation system was only 11 %. Mean yield of digestible dry matter appeared to reach a maximum under a 45-day defoliation interval at 600 kg N/ha but at the lower levels of N the maximum yield was reached at the longest growth interval.Seasonal response to nitrogen under the 22-and 28-day defoliation systems measured as the increase in yield resulting from increased N at each cutting date reached its peak in July-August. Application for these short growth periods early and late in the growing season appeared to be a relatively inefficient use of nitrogen.The less frequently the sward was harvested and the higher the nitrogen application the greater was the reduction in ground cover as estimated by eye at the end of the growing season, this reached an estimated 25% reduction under 112-day defoliation at 300 kg N/ha/year.In relation to published figures nitrate content of herbage did not reach dangerous levels until nitrogen application reached levels beyond those at which maximum dry-matter yield was achieved.