Nitrosative responses in citrus plants exposed to six abiotic stress conditions

Ziogas, Vasileios; Tanou, Georgia; Filippou, Panagiota; Diamantidis, Grigorios; Vasilakakis, M.; Fotopoulos, Vasileios; Molassiotis, Athanassios

doi:10.1016/j.plaphy.2013.04.004

Cited by 107 publications

(63 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although accumulation of NO during drought stress appears to be a general response in diverse plant species and tissues, its specificity has only been established by using various inhibitors / scavengers such as c-PTIO [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide] or L-NAME [(N(G)-nitro-L-arginine-methyl ester] that reverse the process(s) [7,14,17,18,23,25,30,38,39]. Similarly, the kinetics of NO release from donors depends on many factors such as the reactivity of donor, surrounding environment, chemical nature of tissue, light, concentration and active duration of exposure etc.…”

Section: Exogenous No Donors In Drought Stress Ameliorationmentioning

confidence: 99%

See 1 more Smart Citation

NO to drought-multifunctional role of nitric oxide in plant drought: Do we have all the answers?

2015

View full text Add to dashboard Cite

Nitric oxide (NO) is a versatile gaseous signaling molecule with increasing significance in plant research due to its association with various stress responses. Although, improved drought tolerance by NO is associated greatly with its ability to reduce stomatal opening and oxidative stress, it can immensely influence other physiological processes such as photosynthesis, proline accumulation and seed germination under water deficit. NO as a free radical can directly alter proteins, enzyme activities, gene transcription, and post-translational modifications that benefit functional recovery from drought. The present drought-mitigating strategies have focused on exogenous application of NO donors for exploring the associated physiological and molecular events, transgenic and mutant studies, but are inadequate. Considering the biphasic effects of NO, a cautious deployment is necessary along with a systematic approach for deciphering positively regulated responses to avoid any cytotoxic effects. Identification of NO target molecules and in-depth analysis of its effects under realistic field drought conditions should be an upmost priority. This detailed synthesis on the role of NO offers new insights on its functions, signaling, regulation, interactions and co-existence with different drought-related events providing future directions for exploiting this molecule towards improving drought tolerance in crop plants.

show abstract

Section: Exogenous No Donors In Drought Stress Ameliorationmentioning

confidence: 99%

“…The accumulation of NO has often been visualized in guard cells in response to water deficit by using a NO-sensitive fluorescent dye DAF-2DA [10,15,16,23,40]. The increase in NO production correlated significantly to the decrease in stomatal conductance in potted Vitis vinifera under drought stress [22].…”

Section: No As a Modulator Of Stomatal Movementmentioning

confidence: 99%

NO to drought-multifunctional role of nitric oxide in plant drought: Do we have all the answers?

2015

View full text Add to dashboard Cite

show abstract

“…to the ortho position of the aromatic ring of the Tyrosine (Tyr) residue] (Ziogas et al 2013). These protein redox modifications alter the function of a broad spectrum of proteins and therefore the analysis of NO-targeted proteome serves as a tool to investigate the mechanism through which NO impacts their functions (Tanou et al 2010(Tanou et al , 2012b.…”

Section: Introductionmentioning

confidence: 99%

Roles of sodium hydrosulfide and sodium nitroprusside as priming molecules during drought acclimation in citrus plants

Ziogas

Tanou

Belghazi³

et al. 2015

Plant Mol Biol

Self Cite

View full text Add to dashboard Cite

Emerging evidence suggests that the gaseous molecules hydrogen sulfide (H2S) and nitric oxide (NO) enhances plant acclimation to stress; however, the underlying mechanism remains unclear. In this work, we explored if pretreatment of citrus roots with NaHS (a H2S donor) or sodium nitroprusside (SNP, a NO donor) for 2 days (d) could elicit long-lasting priming effects to subsequent exposure to PEG-associated drought stress for 21 d following a 5 d acclimation period. Detailed physiological study documented that both pretreatments primed plants against drought stress. Analysis of the level of nitrite, NOx, S-nitrosoglutahione reductase, Tyr-nitration and S-nitrosylation along with the expression of genes involved in NO-generation suggested that the nitrosative status of leaves and roots was altered by NaHS and SNP. Using a proteomic approach we characterized S-nitrosylated proteins in citrus leaves exposed to chemical treatments, including well known and novel S-nitrosylated targets. Mass spectrometry analysis also enabled the identification of 42 differentially expressed proteins in PEG alone-treated plants. Several PEG-responsive proteins were down-regulated, especially photosynthetic proteins. Finally, the identification of specific proteins that were regulated by NaHS and SNP under PEG conditions provides novel insight into long-term drought priming in plants and in a fruit crop such as citrus in particular.

show abstract

“…Xu et al (2010) observed that NO led to programmed cell death (PCD) in roots of Solanum nigrum suggesting that the effect of NO is dose-dependent. The negative effect of high SNP is the reaction of excess NO with superoxide forming peroxynitrite or other oxidant, which can mediate the tyrosine nitration of proteins, that it can be considered an indicator of nitrosative stress in plants (Chaki et al 2010;Ziogas et al 2013). In our experiment, a low concentration of SNP was used (50 lM), which was proved in the range of protective effect in the past studies (data not shown).…”

Section: Discussionmentioning

confidence: 99%

The time course of NO involved in ABA pathway to improve drought tolerance in Oxytropis ochrocephala Bunge

Chen

et al. 2015

Acta Physiol Plant

View full text Add to dashboard Cite

Oxytropis ochrocephala Bunge is a poisonous legume plant which exhibits drought acclimation behavior and spreads rapidly under adverse environment. This study demonstrates that the stress signals including NO (nitric oxide), ABA (abscisic acid), and H 2 O 2 (Hydrogen peroxide) are involved in roots of O. ochrocephala seedlings when exposed to drought stress simulated by PEG-6000 solution. The relationship among these signals was investigated by using exogenous and endogenous modulators.The results indicate that a time course of NO is accumulated in roots of O. ochrocephala in response to drought stress, which is generated enzymatically by nitrate reductase (NR) activity. The low level of NO acts as a downstream signaling of ABA and is involved with H 2 O 2 signaling cascade. There is a regulatory mechanism of controlling NO concentration and maintaining the equilibrium state between ROS (reactive oxygen species) and NO, which modulates the root cell vitality, and osmotic adjustment thus improves root growth and developmental processes under drought stress.

show abstract

Nitrosative responses in citrus plants exposed to six abiotic stress conditions

Cited by 107 publications

References 40 publications

NO to drought-multifunctional role of nitric oxide in plant drought: Do we have all the answers?

NO to drought-multifunctional role of nitric oxide in plant drought: Do we have all the answers?

Roles of sodium hydrosulfide and sodium nitroprusside as priming molecules during drought acclimation in citrus plants

The time course of NO involved in ABA pathway to improve drought tolerance in Oxytropis ochrocephala Bunge

Contact Info

Product

Resources

About