Nitrosative stress in <i>Escherichia coli</i>: reduction of nitric oxide

Vine, Claire E.; Cole, Jeffrey A.

doi:10.1042/bst0390213

Cited by 41 publications

(46 citation statements)

References 19 publications

(15 reference statements)

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“…2A). By contrast, a small contribution (<3%) from the periplasmatic Nap to NO formation has been reported in both bacteria (Gilberthorpe & Poole, 2008;Rowley et al, 2012;Vine & Cole, 2011).…”

Section: Enzymes Involved In No and N 2 O Metabolismmentioning

confidence: 96%

Nitrous Oxide Metabolism in Nitrate-Reducing Bacteria

Torres

Simon

Rowley

et al. 2016

Advances in Microbial Physiology

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Nitrous oxide (N2O) is an important greenhouse gas (GHG) with substantial global warming potential and also contributes to ozone depletion through photochemical nitric oxide (NO) production in the stratosphere. The negative effects of N2O on climate and stratospheric ozone make N2O mitigation an international challenge. More than 60% of global N2O emissions are emitted from agricultural soils mainly due to the application of synthetic nitrogen-containing fertilizers. Thus, mitigation strategies must be developed which increase (or at least do not negatively impact) on agricultural efficiency whilst decrease the levels of N2O released. This aim is particularly important in the context of the ever expanding population and subsequent increased burden on the food chain. More than two-thirds of N2O emissions from soils can be attributed to bacterial and fungal denitrification and nitrification processes. In ammonia-oxidizing bacteria, N2O is formed through the oxidation of hydroxylamine to nitrite. In denitrifiers, nitrate is reduced to N2 via nitrite, NO and N2O production. In addition to denitrification, respiratory nitrate ammonification (also termed dissimilatory nitrate reduction to ammonium) is another important nitrate-reducing mechanism in soil, responsible for the loss of nitrate and production of N2O from reduction of NO that is formed as a by-product of the reduction process. This review will synthesize our current understanding of the environmental, regulatory and biochemical control of N2O emissions by nitrate-reducing bacteria and point to new solutions for agricultural GHG mitigation.

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Section: Enzymes Involved In No and N 2 O Metabolismmentioning

confidence: 96%

Nitrous Oxide Metabolism in Nitrate-Reducing Bacteria

Torres

Simon

Rowley

et al. 2016

Advances in Microbial Physiology

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“…However, this reduction capacity is low compared with that of NorVW or NrfA. The alternative view is therefore that FNR represses transcription of hmp under anaerobic conditions so that alternative, more effective, protection mechanisms are activated anaerobically (Vine & Cole, 2011a). It is possible, however, that Hmp synthesis becomes physiologically relevant under extreme environmental conditions.…”

Section: Repair Of Nitrosative Damagementioning

confidence: 99%

Legless pathogens: how bacterial physiology provides the key to understanding pathogenicity

Cole

2012

Microbiology

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“…Although the importance of these reactive nitrogen and oxygen intermediate species has been documented for host defense reactions against bacteria and fungi, their role in the pathogenesis of viral infections is only partly understood [9,10]. It has been reported that nitric oxide (NO) has deleterious consequences in the presence of reactive oxygen species, since peroxynitrites are formed.…”

Section: Introductionmentioning

confidence: 99%

Downregulation of inducible nitric oxide synthase (iNOS) expression is implicated in the antiviral activity of acetylsalicylic acid in HCV-expressing cells

et al. 2014

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Previously, we described that acetylsalicylic acid (ASA) decreases HCV expression, but the mechanisms involved have not been clearly established. We evaluated the participation of inducible nitric oxide synthase (iNOS) in the regulation of HCV-RNA induced by ASA. Huh7 cells expressing non-structural HCV proteins were exposed to 4 mM ASA and incubated at the same times we reported HCV downregulation (24-72 h), and iNOS mRNA and protein levels were then measured by real-time PCR and Western blot, respectively. Nitric oxide levels were measured at the same time. Inhibition of iNOS mRNA by small interfering RNAs (siRNA) and activation of the iNOS gene promoter by ASA treatment were evaluated. In Huh7 replicon cells treated with ASA, we found decreased levels of iNOS mRNA, iNOS protein and nitrosylated protein levels at 48-72 h. ASA exposure also reduced the transactivation of the iNOS promoter in HCV replicon cells at 48 h, and this was partly due to the decrease in the affinity of transcription factor C/EBP-β for its binding site in the iNOS promoter. siRNA silencing of iNOS decreased HCV-RNA expression (65 %) and potentiated the antiviral effect (80 %) of ASA compared with control cells. ASA reduces iNOS expression by downregulating promoter activity, mRNA and protein levels at the same time that it decreases HCV expression. These findings suggest that the antiviral activity of ASA is mediated partially through the modulation of iNOS.

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Nitrosative stress in Escherichia coli: reduction of nitric oxide

Cited by 41 publications

References 19 publications

Nitrous Oxide Metabolism in Nitrate-Reducing Bacteria

Nitrous Oxide Metabolism in Nitrate-Reducing Bacteria

Legless pathogens: how bacterial physiology provides the key to understanding pathogenicity

Downregulation of inducible nitric oxide synthase (iNOS) expression is implicated in the antiviral activity of acetylsalicylic acid in HCV-expressing cells

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