Nitrosoguanidine Mutagenesis and Genome Shuffling Enhanced the Oxytetracycline Production of Streptomyces rimosus

Lu, Zhitang

doi:10.9790/3008-0462327

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…Hay varios ejemplos en la literatura en el que se ha usado un agente mutagénico en una cepa parental, seguida de diversas rondas de genome shuffling para obtener cepas mejoradas; por ejemplo, en el caso de la cepa Saccharopolyspora spinosa donde con UV diversificaron a su cepa parental de alta producción, y mediante 4 rondas de Genome Shuffling lograron obtener una cepa que produjo 4.36 veces más que a cepa parental (Zhihua Jin, Cheng, & Cen, 2006); para la cepa Streptomyces avermitilis iniciaron diversificando la cepa con metilnitronitrosoguanidina (MNNG) y UV, utilizando estreptomicina como elemento de selección y finalmente realizaron una ronda de Genome Shuffling logrando producir 11.2 veces más que la cepa parental en cuanto a la producción de doramectina ; para una cepa de Streptomyces rimosus productora de oxitetraciclina se diversificaron las poblaciones con MNNG, estas se recombinaron con 6 rodas de Genome Shuffling, y se obtuvo una cepa capaz de producir 339% más que la cepa parental (Lu, Zhang, Liu, & Li, 2013). Para la cepa…”

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Desarrollo de un sistema para el mejoramiento genético de Streptomyces sp

Rodríguez

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Section: Selección Secundaria De Fusionantesunclassified

Desarrollo de un sistema para el mejoramiento genético de Streptomyces sp

Rodríguez

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An Insight into the “-Omics” Based Engineering of Streptomycetes for Secondary Metabolite Overproduction

Chaudhary

Dhakal

Sohng

2013

BioMed Research International

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Microorganisms produce a range of chemical substances representing a vast diversity of fascinating molecular architectures not available in any other system. Among them, Streptomyces are frequently used to produce useful enzymes and a wide variety of secondary metabolites with potential biological activities. Streptomyces are preferred over other microorganisms for producing more than half of the clinically useful naturally originating pharmaceuticals. However, these compounds are usually produced in very low amounts (or not at all) under typical laboratory conditions. Despite the superiority of Streptomyces, they still lack well documented genetic information and a large number of in-depth molecular biological tools for strain improvement. Previous attempts to produce high yielding strains required selection of the genetic material through classical mutagenesis for commercial production of secondary metabolites, optimizing culture conditions, and random selection. However, a profound effect on the strategy for strain development has occurred with the recent advancement of whole-genome sequencing, systems biology, and genetic engineering. In this review, we demonstrate a few of the major issues related to the potential of “-omics” technology (genomics, transcriptomics, proteomics, and metabolomics) for improving streptomycetes as an intelligent chemical factory for enhancing the production of useful bioactive compounds.

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Nitrosoguanidine Mutagenesis and Genome Shuffling Enhanced the Oxytetracycline Production of Streptomyces rimosus

Cited by 2 publications

References 15 publications

Desarrollo de un sistema para el mejoramiento genético de Streptomyces sp

Desarrollo de un sistema para el mejoramiento genético de Streptomyces sp

An Insight into the “-Omics” Based Engineering of Streptomycetes for Secondary Metabolite Overproduction

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