Nitrosomonas sp. Based biosensor for ammonium nitrogen measurement in wastewater

Raud, Merlin; Lember, Erki; Jõgi, Eerik; Kikas, Timo

doi:10.1007/s12257-013-0078-x

Cited by 13 publications

(6 citation statements)

References 28 publications

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“…High contents of organic load as well as the high proliferation rate of microorganisms are considered as the causative factors for high BOD in water. Shallow ponds are efficient in removing BOD because of their low surface loading (Raud et al 2013). In this study, we observed seasonal variations in the levels of 33 mg/L) and COD (39.33-354.66 mg/L); the high BOD level during summer may be attributed to the increased metabolic activity of microbes.…”

Section: Discussionmentioning

confidence: 54%

“…Suspected cultures were evaluated for presence of ammonia and nitrite and vice versa for production of nitrite and nitrate (Saha et al 2014;Roalkvam et al 2020). Identification of Nitrosomonas was tested by the Nessler's reagent (Raud et al 2013;Agus Fitri et al 2017) and Nitrosomonas and Nitrobacter were confirmed through Trommsdrof's reagent (Hanks & Weintraub 1936;Kshatri et al 2017). Selected cultures were preserved at À80°C with 30-40% glycerol stock for further studies.…”

Section: Bacteriological Analysesmentioning

confidence: 99%

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Water quality assessment of East Kolkata Wetland with a special focus on bioremediation by nitrifying bacteria

Saha

Sarkar

Bandyopadhyay

2021

Water Science and Technology

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East Kolkata Wetlands (EKW) is designated as International Ramsar site and are the hotspot for large-scale wastewater aquaculture practices. However, the continued surveillance of physicochemical properties of water and application of an eco-friendly approach are essential to ensure safe aquaculture practices. In the present study, we assessed the seasonal variation in physicochemical parameters of water across EKW and investigated the role of nitrifying bacteria as probiotics. We statistically analyzed various physicochemical properties of water samples from EKW. Results of the statistical analysis indicated a significant variation in all the physicochemical parameters across the selected water bodies of EKW (p < 0.01). We isolated and enumerated Nitrosomonas sp. and Nitrobacter sp. and assessed their ability to degrade trichloroethylene (TCE). The role of Nitrosomonas and Nitrobacter sp. were further investigated and established through a small-scale experiment. Two microbial isolates, NSW3 and NBW2, displayed superior TCE degradation ability at pH 5, and the application of these strains as probiotics were found to improve the quality of water and survival rate of fishes in the treated experimental tanks. Our findings suggest that the application of the above mixed bacterial cultures in aquaculture could be an effective and environment-friendly approach for safe and productive aquaculture operations.

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Section: Discussionmentioning

confidence: 54%

Section: Bacteriological Analysesmentioning

confidence: 99%

Water quality assessment of East Kolkata Wetland with a special focus on bioremediation by nitrifying bacteria

Saha

Sarkar

Bandyopadhyay

2021

Water Science and Technology

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“…Due to their ability to oxidize NH 3 , Nitrosomonas sp. also are used for the development of biosensors for estimation of ammonium‐N in wastewater 34 . Conversion of CH 4 produced from biogas plants can have beneficial impacts on the performance of wastewater treatment.…”

Section: Resultsmentioning

confidence: 99%

Continuous culture of Nitrosomonas eutropha and biomethanol production using whole cell biotransformation

Sabale

Babu

Panchwagh

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND Nitrosomonas eutropha is a chemolithoautotrophic organism with an ability to oxidize ammonium to nitrite and nitrate. Continuous culture with and without cell recycling was used to enhance production of N. eutropha cell biomass using a 40‐L bioreactor. Whole cell biotransformation was used to convert methane (CH4) present in the biogas into biomethanol using N. eutropha. RESULTS The maximum biomass production rate of N. eutropha was increased by three‐fold with provision of cell recycling in the continuous culture. Removal of nitrite from the continuous culture resulted in high activity of ammonia monooxygenase enzyme responsible for conversion of CH4 to biomethanol. Use of ammonium sulfate as a source of electrons for biotransformation reaction with N. eutropha resulted in a maximum 119.86 mg L−1 of biomethanol production. CONCLUSION Our research provides an efficient process for biomass production of N. eutropha using continuous culture. We have demonstrated improvement in CH4 to biomethanol conversion using biogas as feedstock. © 2021 Society of Chemical Industry

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“… Amperometric 1.4 × 10 −4 –1.43 × 10 −3 – 0.030–0.036 n.s.r. 1.4 × 10 −4 15–25 min (in 5–6 days 10–15 min) 14 days [ 17 ] SiO 2 /ZrO 2 /phosphate-NH 4 composite Potentiometric 1.0 × 10 −6 –1.0 × 10 −2 7.7 × 10 −7 –4.0 × 10 −2 31.3 mV decade −1 1.58 × 10 −7 1 min 6 months [ 18 ] Poly(vinyl chloride) membrane with nonactin Potentiometric 1.0 × 10 −5 –1.0 × 10 −1 – 56 mV decade −1 8.0 × 10 −6 20 s 6 months [ 19 ] Zirconium titanium phosphate ion exchanger Potentiometric 1.0 × 10 −4 –1 1.2 × 10 −5 –1 42 mV decade −1 1.0 × 10 −5 30 s Over 2 years (if stored under controlled conditions). [ 20 ] Copper ion-doped clinoptilolite Amperometric 2.0 × 10 −5 –1.0 × 10 −3 2.0 × 10 −5 –1.0 × 10 −2 191 μA mol −1 L 5.0 × 10 −6 1 min – [ 21 ] 25,27-di-(5-thio-octyloxy)calix[4]arene-crown-6 Conductometric 1 × 10 −5 –1.5 × 10 −3 1.0 × 10 −5 –1.0 × 10 −2 8.22 μs mM −1 1.0 × 10 −5 5–10 s 115 days This work PANI polyaniline, PSSMA poly(styrene sulfonate- co -maleic acid) anion, n.s.r.…”

Section: Resultsmentioning

confidence: 99%

Development of Conductometric Sensor Based on 25,27-Di-(5-thio-octyloxy)calix[4]arene-crown-6 for Determination of Ammonium

et al. 2016

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The conductometric sensor based on 25,27-di-(5-thio-octyloxy)calix[4]arene-crown-6 was developed for the quantitative analysis of ammonium. The calixarene was immobilized on the surface of the planar interdigitated electrodes by attachment of its dialkyl sulfide groups to the surface of the gold electrodes. The intrinsic ability of the calixarene to capture ammonium was studied in the conductometric measuring mode and by the electrochemical impedance spectroscopy. The developed sensor showed high selectivity to ammonium in the presence of mono-, di-, and trivalent cations. Selective and highly sensitive detection of ammonium resulted from the complexation between the ammonium ions and a crown-ether fragment of the upper rim of the 25,27-di-(5-thio-octyloxy)calix[4]arene-crown-6 macrocycle. The developed sensor had high signal repeatability. Its sensitivity was found to be satisfactory for the forthcoming sensor application in the water-sample analysis; the linear range was 0.01–1.5 mM and limit of detection 10 μM.Electronic supplementary materialThe online version of this article (doi:10.1186/s11671-016-1317-9) contains supplementary material, which is available to authorized users.

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Nitrosomonas sp. Based biosensor for ammonium nitrogen measurement in wastewater

Cited by 13 publications

References 28 publications

Water quality assessment of East Kolkata Wetland with a special focus on bioremediation by nitrifying bacteria

Water quality assessment of East Kolkata Wetland with a special focus on bioremediation by nitrifying bacteria

Continuous culture of Nitrosomonas eutropha and biomethanol production using whole cell biotransformation

Development of Conductometric Sensor Based on 25,27-Di-(5-thio-octyloxy)calix[4]arene-crown-6 for Determination of Ammonium

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