Nitrous Oxide Consumption in Oxygenated and Anoxic Estuarine Waters

Tang, Wanhu; Jayakumar, Amal; Sun, Xin; Tracey, John C.; Carroll, Julia; Wallace, Elizabeth; Lee, Jenna; Nathan, Levy; Ward, Bess B.

doi:10.1029/2022gl100657

Cited by 10 publications

(6 citation statements)

References 54 publications

(87 reference statements)

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“…However, in estuarine and coastal areas of China, Clade I nosZ was transcribed more actively than Clade II, despite being less abundant (Dai et al, 2022 ). Chesapeake Bay qPCR measurements show Clade II as ~2 orders of magnitude more abundance than Clade I, suggesting it dominates N 2 O consumption (Tang et al, 2022 ). Therefore, the two nosZ clades have varying biochemical impacts on N 2 O consumption, likely dependent on their environmental source.…”

Section: Discussionmentioning

confidence: 99%

“…Two phylogenetically distinct variants of nosZ have been identified: Clade I and II, also known as typical and atypical, respectively (Jones et al, 2012 ; Sanford et al, 2012 ). Differences in kinetics (Yoon et al, 2016 ; Tang et al, 2022 ), community composition, and/or environmental distribution between Clade I and II containing organisms are likely to impact environmental N 2 O fluxes. Clade I organisms are often found to also contain genes encoding the enzymes for complete denitrification.…”

Section: Introductionmentioning

confidence: 99%

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Aquatic nitrous oxide reductase gene (nosZ) phylogeny and environmental distribution

Intrator,

Jayakumar,

Ward

2024

Front. Microbiol.

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Nitrous oxide (N2O) is a potent greenhouse gas and a major cause of ozone depletion. One-third of atmospheric N2O originates in aquatic environments. Reduction of N2O to dinitrogen gas (N2) requires the nitrous oxide reductase enzyme, which is encoded by the gene nosZ. Organisms that contain nosZ are the only known biological sinks of N2O and are found in diverse genera and a wide range of environments. The two clades of nosZ (Clade I and II) contain great diversity, making it challenging to study the population structure and distribution of nosZ containing organisms in the environment. A database of over 11,000 nosZ sequences was compiled from NCBI (representing diverse aquatic environments) and unpublished sequences and metagenomes (primarily from oxygen minimum zones, OMZs, where N2O levels are often elevated). Sequences were clustered into archetypes based on DNA and amino acid sequence identity and their clade, phylogeny, and environmental source were determined. Further analysis of the source and environmental distribution of the sequences showed strong habitat separation between clades and phylogeny. Although there are more Clade I nosZ genes in the compilation, Clade II is more diverse phylogenetically and has a wider distribution across environmental sources. On the other hand, Clade I nosZ genes are predominately found within marine sediment and are primarily from the phylum Pseudonomonadota. The majority of the sequences analyzed from marine OMZs represented distinct phylotypes between different OMZs showing that the nosZ gene displays regional and environmental separation. This study expands the known diversity of nosZ genes and provides a clearer picture of how the clades and phylogeny of nosZ organisms are distributed across diverse environments.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Aquatic nitrous oxide reductase gene (nosZ) phylogeny and environmental distribution

Intrator,

Jayakumar,

Ward

2024

Front. Microbiol.

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“…Inter-lake differences in N 2 O emissions have been reported for Finnish lakes (Huttunen et al, 2003a;Kortelainen et al, 2020) and Chinese lakes (Zhou et al, 2021), and N 2 O emissions are higher in lakes with higher trophic levels. The roles of nitrification and denitrification in determining dissolved N 2 O concentrations and N 2 O emissions have also been reported (e.g., Yoh et al, 1983;Mengis et al, 1997;Senga et al, 2002;Beaulieu et al, 2014;Salk et al, 2016;Lian et al, 2022;Tang et al, 2022a, b).…”

Section: Introductionmentioning

confidence: 86%

“…reduction obtained in incubation experiments byTang et al (2022a) for the initial N 2 O concentration. This implies that some of the N 2 O accumulated in the bottom water layer was diffused to the upper water layer under enhanced lake water mixing due to the continuously higher wind speeds during the afternoon.…”

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confidence: 99%

Sub-daily Variations in Nitrous Oxide Fluxes from the Littoral Zone of a Temperate Eutrophic Lake

Iwata,

Kawagishi,

Takahashi

et al. 2024

Preprint

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Lakes are sources of nitrous oxide (N2O). However, labor-intensive gas analysis has confined evaluations of temporal variability in lakes to the seasonal time scale. We used a state-of-the-art laser-based N2O analyzer combined with a floating chamber to examine sub-daily variations in N2O emissions from a shallow eutrophic lake in Japan. We conducted intense daytime field campaigns during different seasons to reveal differences in sub-daily variations in N2O emissions from the water surface in conjunction with the dynamics of dissolved N2O concentrations in the lake water. The study revealed that N2O fluxes varied within a day: emissions increased in response to increased wind speeds. Variations in surface dissolved N2O concentrations caused by water mixing were also important during the summer, when accumulation of N2O initially occurred in the lake bottom layer during stably stratified conditions. During winter, the lake water was well mixed and dissolved N2O concentrations were uniform throughout the water column; thus, wind speed was the only factor controlling the diurnal variations in N2O emissions. These findings demonstrate the need to consider diurnal variability when estimating cumulative N2O emissions accurately over long periods.

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“…The potential for N 2 O consumption or depletion exists even in oxic waters (Rees et al., 2021; X. Sun et al., 2021; Tang et al., 2022; Toyoda et al., 2021). To date, the biogeochemical cycle of N 2 O in ocean water and sediment includes ammonia oxidation (ammonia‐oxidizing bacteria [AOB]) (J. H. Guo et al., 2013), ammonia oxidation process (ammonia‐oxidizing archaea [AOA]) (L. Wu et al., 2020), nitrifier denitrification process (Sedlacek et al., 2020), dissimilatory nitrate/nitrite reduction to ammonium (Y. H. Sun et al., 2016), algal photochemical process (Guieysse et al., 2013), fungal metabolism (Peng & Valentine, 2021) hybrid formation (Stieglmeier et al., 2014; Wan et al., 2023) and abiological process catalyzed by iron‐based and manganese‐based catalysts (Zhu‐Barker et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

Potential Contributions of Ammonia‐Oxidizing Microorganisms to the Distributions of Nitrous Oxide in the Northern Bering Sea

Liu,

Chen,

Ling

et al. 2024

JGR Oceans

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Oceanic N2O is a major source of atmospheric N2O gas and is involved in global warming and ozone depletion. It is thought to be mainly produced by nitrification, denitrification and nitrifier denitrification processes mediated by ammonia‐oxidizing bacteria, ammonia‐oxidizing archaea (AOA) and denitrifying bacteria. The Bering Sea, especially its continental shelf area, is considered a typical source of atmospheric N2O. During the 7th Chinese National Arctic Research Expedition (CHINARE2016), the distributions of N2O and ammonia‐oxidizing microorganisms (AOOs) in the Bering Sea continental shelf and abyssal basin water were investigated. At a depth of 50∼900 m within the abyssal basin, the in‐situ ammonia oxidation process, particularly performed by AOA, exhibits considerable potential for the formation of supersaturated N2O. Meanwhile, beneath the oxygen minimum zone (depth range is approximately 800∼1,000 m), supersaturated N2O is primarily driven by mixing processes, while the ammonia oxidation mediated by AOA also contributes to a certain extent. In addition, the N2O distribution characteristic exhibits a substantial disparity between the southern and northern Bering Sea shelves, with the former characterized as a mild sink and the latter as a weak source. The water column of the Bering Sea demonstrates a considerable potential for generating supersaturated N2O through ammonia oxidation, as corroborated by the current study.

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Nitrous Oxide Consumption in Oxygenated and Anoxic Estuarine Waters

Cited by 10 publications

References 54 publications

Aquatic nitrous oxide reductase gene (nosZ) phylogeny and environmental distribution

Aquatic nitrous oxide reductase gene (nosZ) phylogeny and environmental distribution

Sub-daily Variations in Nitrous Oxide Fluxes from the Littoral Zone of a Temperate Eutrophic Lake

Potential Contributions of Ammonia‐Oxidizing Microorganisms to the Distributions of Nitrous Oxide in the Northern Bering Sea

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