Nitrous oxide (N2O)-reducing denitrifier-inoculated organic fertilizer mitigates N2O emissions from agricultural soils

Gao, Nan; Shen, Weishou; Camargo, Estefânia Silva; Shiratori, Yutaka; Nishizawa, Tomoyasu; Isobe, Kazuo; He, Xinhua; Senoo, Keishi

doi:10.1007/s00374-017-1231-z

Cited by 30 publications

(11 citation statements)

References 46 publications

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“…Further efforts should be directed towards selecting organisms that are both strong sinks for N 2 O and able to survive and compete in soil, to secure long-lasting effects on N 2 O emissions. A tantalizing added value would be provided by selecting organisms (or consortia of organisms) that are not only strong N 2 O-sinks, but also promote plant growth and disease resistance [38,39].…”

Section: Discussionmentioning

confidence: 99%

Nitrous oxide respiring bacteria in biogas digestates for reduced agricultural emissions

et al. 2021

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Inoculating agricultural soils with nitrous oxide respiring bacteria (NRB) can reduce N2O-emission, but would be impractical as a standalone operation. Here we demonstrate that digestates obtained after biogas production are suitable substrates and vectors for NRB. We show that indigenous NRB in digestates grew to high abundance during anaerobic enrichment under N2O. Gas-kinetics and meta-omic analyses showed that these NRB’s, recovered as metagenome-assembled genomes (MAGs), grew by harvesting fermentation intermediates of the methanogenic consortium. Three NRB’s were isolated, one of which matched the recovered MAG of a Dechloromonas, deemed by proteomics to be the dominant producer of N2O-reductase in the enrichment. While the isolates harbored genes required for a full denitrification pathway and could thus both produce and sequester N2O, their regulatory traits predicted that they act as N2O sinks in soil, which was confirmed experimentally. The isolates were grown by aerobic respiration in digestates, and fertilization with these NRB-enriched digestates reduced N2O emissions from soil. Our use of digestates for low-cost and large-scale inoculation with NRB in soil can be taken as a blueprint for future applications of this powerful instrument to engineer the soil microbiome, be it for enhancing plant growth, bioremediation, or any other desirable function.

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Section: Discussionmentioning

confidence: 99%

Nitrous oxide respiring bacteria in biogas digestates for reduced agricultural emissions

et al. 2021

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“…Further efforts should be on selecting organisms that are both strong sinks for N2O, and able to survive in soil, in order to secure a long-lasting effect on the soils propensity to emit N2O. A tantalizing option which may offset some of the economic costs associated with such a process is to select organisms (or consortia of organisms) that are not only strong N2O sinks, but also promote plant growth and disease resistance (Gao et al 2016(Gao et al , 2017.…”

Section: Conclusive Remarksmentioning

confidence: 99%

N₂O-respiring bacteria in biogas digestates for reduced agricultural emissions

et al. 2020

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Mitigation of N2O-emissions from soils is needed to reduce climate forcing by food production. Inoculating soils with N2O-reducing bacteria would be effective, but costly and impractical as a standalone operation. Here we demonstrate that digestates obtained after biogas production may provide a low-cost and widely applicable solution. Firstly, we show that indigenous N2O-reducing bacteria in digestates grow to high levels during anaerobic enrichment under N2O. Gas kinetics and meta-omic analysis show that the N2O-respiring organisms, recovered as metagenome-assembled genomes (MAGs), grow by harvesting fermentation intermediates of the methanogenic consortium. Three digestate-derived denitrifying, N2O-reducing bacteria were obtained through isolation, one of which matched the recovered MAG of a dominant Dechloromonas-affiliated N2O reducer. While the identified N2O-reducers encoded genes required for a full denitrification pathway and could thus both produce and sequester N2O, their regulatory traits predicted that they act as N2O-sinks. Secondly, moving towards practical application, we show that these isolates grow by aerobic respiration in digestates, and that fertilization with these enriched digestates reduces N2O emissions. This shows that the ongoing implementation of biogas production in agriculture opens a new avenue for cheap and effective reduction of N2O emissions from food production.

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“…Plant growth-promoting rhizobacteria (PGPR) are a group of rhizosphere bacteria which can improve plant growth, suppress invading pathogens, and improve plant abiotic stress tolerance and productivity (1–4). Studies have shown that the inoculation of soil with PGPR strains with nitrous oxide (N 2 O)-reducing ability decreases N 2 O emissions (2, 5, 6). As PGPR, Azospirillum sp.…”

Section: Announcementmentioning

confidence: 99%

“…As PGPR, Azospirillum sp. strains TSA2S and TSH100, originally isolated from the rhizosphere of paddy soil in Tokyo, Japan (7), possess the ability to improve plant growth and mitigate N 2 O from soil (2, 6). Thus, these two strains could be explored as environmentally friendly biofertilizers.…”

Section: Announcementmentioning

confidence: 99%

Genome Sequences of Two Azospirillum sp. Strains, TSA2S and TSH100, Plant Growth-Promoting Rhizobacteria with N ₂ O Mitigation Abilities

Gao

Shen

Nishizawa

et al. 2019

Microbiol Resour Announc

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Nitrous oxide (N2O)-reducing denitrifier-inoculated organic fertilizer mitigates N2O emissions from agricultural soils

Cited by 30 publications

References 46 publications

Nitrous oxide respiring bacteria in biogas digestates for reduced agricultural emissions

Nitrous oxide respiring bacteria in biogas digestates for reduced agricultural emissions

N₂O-respiring bacteria in biogas digestates for reduced agricultural emissions

Genome Sequences of Two Azospirillum sp. Strains, TSA2S and TSH100, Plant Growth-Promoting Rhizobacteria with N ₂ O Mitigation Abilities

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Nitrous oxide (N2O)-reducing denitrifier-inoculated organic fertilizer mitigates N2O emissions from agricultural soils

Cited by 30 publications

References 46 publications

Nitrous oxide respiring bacteria in biogas digestates for reduced agricultural emissions

Nitrous oxide respiring bacteria in biogas digestates for reduced agricultural emissions

N2O-respiring bacteria in biogas digestates for reduced agricultural emissions

Genome Sequences of Two Azospirillum sp. Strains, TSA2S and TSH100, Plant Growth-Promoting Rhizobacteria with N 2 O Mitigation Abilities

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Product

Resources

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N₂O-respiring bacteria in biogas digestates for reduced agricultural emissions

Genome Sequences of Two Azospirillum sp. Strains, TSA2S and TSH100, Plant Growth-Promoting Rhizobacteria with N ₂ O Mitigation Abilities