Nitroxides and a nitroxide-based UV filter have the potential to photoprotect UVA-irradiated human skin fibroblasts against oxidative damage

Venditti, Elisabetta; Brugè, Francesca; Astolfi, Paola; Kochevar, Irene E.; Damiani, Elisabetta

doi:10.1016/j.jdermsci.2011.03.008

Cited by 24 publications

(16 citation statements)

References 65 publications

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“…However, the viability of post-irradiation cultures exceeded 90 during 72 h-incubation period Fig.3 B , suggesting that the UV-A doses caused growth inhibition, rather than cell death caused by plasma membrane damage and apoptosis by oxidative stress. Another report Venditti et al, 2011 showed that when fibroblasts were irradiated with 9 J cm -2 or 18 J cm -2 , 95 and 75 of the cells were viable, respectively, corresponding well with the viabilities determined in this study.…”

Section: Effect Of a Polyphenol On Cell Proliferation After Uv-a Irrasupporting

confidence: 89%

Effect of Polyphenols on Reactive Oxygen Species Production and Cell Growth of Human Dermal Fibroblasts after Irradiation with Ultraviolet-A Light

et al. 2015

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Ultraviolet-A UV-A can damage microbes by generating reactive oxygen species ROS , singlet oxygen, superoxides, hydrogen peroxide and hydroxyl radicals. These species readily react with lipids, proteins, DNA and other constituents of cells, leading to oxidative deterioration and the eventual death of the microbe. However, the oxidative ability of these reactive species also harms the viability of mammalian cells such as fibroblasts and keratinocytes, as they cause both acute and chronic damage, photo-aging, and photo-carcinogenesis. This study describes a UV-A treatment that does not affect the viability or growth of human neonate dermal fibroblasts, as determined by examining the post-irradiation cell density after the addition of polyphenols as antioxidants. The results demonstrate the possible wide applicability of UV-A sterilization. The potency of polyphenols for attenuating UV-A-induced ROS generation in cells was tested using -catechin hydrate, -epigallocatechin gallate hydrate, morin hydrate, quercetin hydrate and resveratrol. The lowest concentration of polyphenols required to reduce ROS by 50 in cells upon exposure to a dose of 15 J cm -2 was determined and defined as its IC 50 . Pre-treatment with morin hydrate at its IC 50 allowed cells irradiated with 5.0 J cm -2 UV-A to recover to the level of the specific growth rate of cells incubated without UV-A irradiation. However, the growth rate of cells exposed to 15 J cm -2 UV-A irradiation was scarcely influenced by co-incubation with morin hydrate this dose of UV-A also suppressed cell growth completely in the absence of morin hydrate, although co-incubation resulted in no decrease in cell viability. This study demonstrates the potential of polyphenols for protecting both the viability of cells and their ability to proliferate from damage caused by UV-A-irradiation.

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Section: Effect Of a Polyphenol On Cell Proliferation After Uv-a Irrasupporting

confidence: 89%

Effect of Polyphenols on Reactive Oxygen Species Production and Cell Growth of Human Dermal Fibroblasts after Irradiation with Ultraviolet-A Light

et al. 2015

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“…These effects of EEDO were mediated by inhibition of ROS generation, a key factor in premature senescence induced by UVB irradiation. 11,12) Treatment of EEDO to HR-1 hairless mice exposed to UVB significantly attenuated the number of senescent cells and reduced ROS levels in the skin.…”

Section: Discussionmentioning

confidence: 99%

“…2,10) Excess production and ineffective elimination of ROS production have thus been implicated in skin photoaging induced by UV radiation. 11,12) Dalbergia odorifera T. Chem (Leguminosae) is an indigenous medicinal herb that has been widely used in China and Korea for the treatment of ischemia, swelling, necrosis, rheumatic pain, and blood disorders. 13) Several components from the heartwood of D. odorifera, including flavonoids, quinines, and phenolic constituents, exhibit diverse biological activities.…”

mentioning

confidence: 99%

Ethanol extract of Dalbergia odorifera protects skin keratinocytes against ultraviolet B-induced photoaging by suppressing production of reactive oxygen species

Ham

Hwang

Kang

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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“…A tenfold increase in MMP-1 mRNA expression levels was observed 24 h post-UVA treatment, which was significantly reduced by all nitroxide compounds (Venditti et al 2011). …”

Section: Othersmentioning

confidence: 93%

Antioxidants and Skin Aging

Hashimoto

2014

Systems Biology of Free Radicals and Antioxidants

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Nitroxides and a nitroxide-based UV filter have the potential to photoprotect UVA-irradiated human skin fibroblasts against oxidative damage

Cited by 24 publications

References 65 publications

Effect of Polyphenols on Reactive Oxygen Species Production and Cell Growth of Human Dermal Fibroblasts after Irradiation with Ultraviolet-A Light

Effect of Polyphenols on Reactive Oxygen Species Production and Cell Growth of Human Dermal Fibroblasts after Irradiation with Ultraviolet-A Light

Ethanol extract of Dalbergia odorifera protects skin keratinocytes against ultraviolet B-induced photoaging by suppressing production of reactive oxygen species

Antioxidants and Skin Aging

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