NKT Cell Stimulation with α-Galactosylceramide Results in a Block of Th17 Differentiation after Intranasal Immunization in Mice

Zygmunt, Beata M.; Weißmann, Sebastian; Guzmán, Carlos A.

doi:10.1371/journal.pone.0030382

Cited by 11 publications

(11 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, a-galactosylceramide, which was used to mobilize and activate iNKT (16,19), was reported to protect mice against the development of CVB3 inducedmyocarditis (21). In addition, it is proved that a-galactosylceramide could not only stimulate NKT but also result in a block of Th17 differentiation after intranasal immunization in mice (24). Our previous study reveals that Th17 could aggravate CVB3-induced myocarditis (22).…”

Section: Discussionmentioning

confidence: 97%

The Frequency of Invariant Natural Killer T Cells Correlates with the Severity of Myocarditis

Wang

et al. 2014

Viral Immunology

View full text Add to dashboard Cite

Invariant natural killer T cells (iNKT) perform different functions in different diseases. The cells were reported to protect myocarditis. However, the detail relationships between iNKT and Coxsackievirus B3 (CVB3)-induced myocarditis remain unclear. In order to investigate the correlation between the severity of CVB3-induced inflammation infiltration and the proportion of iNKT in the spleen and circulating blood, BALB/c mice were grouped into three groups according to the inflammation infiltration area of heart sections. The proportion of iNKT in CD3-positive cells in the spleen correlated negatively with the inflammation area (linear fit; R(2)=0.93) and virus capsid protein VP1 (linear fit; R(2)=0.84) in the myocardial tissue, while the proportion of iNKT in CD3-positive cells in the PBMC positively correlated with the inflammation area (linear fit; R(2)=0.91) and virus capsid protein VP1 (linear fit; R(2)=0.93) in the myocardial tissue. The results imply that iNKT might be used as a parameter for the diagnosis of myocarditis in clinical practice.

show abstract

Section: Discussionmentioning

confidence: 97%

The Frequency of Invariant Natural Killer T Cells Correlates with the Severity of Myocarditis

Wang

et al. 2014

Viral Immunology

View full text Add to dashboard Cite

show abstract

“…Although phytosphingosine exhibited beneficial effects in cosmetic markets, topical application at high doses is limited because of high toxicity. Several researchers modified and developed phytosphingosine derivatives to reduce toxic effects, and they examined the biological activities of these compounds (Kim et al, 2003a(Kim et al, , b, 2004Park et al, 2003;Pavicic et al, 2007;Ryu et al, 2010;Walsh et al, 2011;Paget et al, 2012;Zygmunt et al, 2012). The biological and pharmacological activities of YG-II-6 compounds were tested in an animal model, in which skin inflammation was induced by topical application of TPA or intradermal injection of IL-23 in mouse skin.…”

Section: Discussionmentioning

confidence: 99%

“…Phytosphingosine is a class of sphingolipids that regulate the T cell-mediated immune response (Walsh et al, 2011;Paget et al, 2012;Zygmunt et al, 2012). The cytokine-mediated JAK/STAT signaling pathway also regulates the immune response (Shuai and Liu, 2003).…”

Section: Yg-ii-6 Compounds Inhibit Jak/stat Signalingmentioning

confidence: 99%

Phytosphingosine Derivatives Ameliorate Skin Inflammation by Inhibiting NF-κB and JAK/STAT Signaling in Keratincoytes and Mice

Kim

Lee

Jung

et al. 2014

Journal of Investigative Dermatology

View full text Add to dashboard Cite

Phytosphingosine is abundant in plants and fungi and is found in mammalian epidermis, including the stratum corneum. Phytosphingosine and its derivatives N-acetyl phytosphingosine and tetraacetyl phytosphingosine are part of the natural defense system of the body. However, these molecules exhibit strong toxicities at high concentrations. We synthesized phytosphingosine derivatives, mYG-II-6 ((Z)-4-oxo-4-(((2S,3S,4R)-1,3,4-trihydroxyoctadecan-2-yl)amino)but-2-enoic acid) and fYG-II-6 ((E)-4-oxo-4-(((2S,3S,4R)-1,3,4-trihydroxyoctadecan-2-yl)amino)but-2-enoic acid), to increase efficacy and decrease toxicity, and the biological activities of the derivatives in the inflammatory response were examined. Both YG-II-6 compounds effectively suppressed 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammatory skin damage and inflammatory response in a mouse model. In addition, topical application of fYG-II-6 suppressed ear swelling and psoriasiform dermatitis in the ears of IL-23-injected mice. Anti-inflammatory and antipsoriatic activities of the phytosphingosine derivatives inhibited NF-κB, JAK/signal transducer and activator of transcription (JAK/STAT), and mitogen-activated protein kinase (MAPK) signaling. Finally, the YG-II-6 compounds induced programmed cell death in keratinocytes and mouse skin and were less toxic than phytosphingosine. Our study demonstrated that the phytosphingosine-derived YG-II-6 compounds have much stronger biological potencies than the lead compounds. The YG-II-6 compounds ameliorated inflammatory skin damage. Thus, YG-II-6 compounds are potential topical agents for treating chronic inflammatory skin diseases, such as psoriasis.

show abstract

“…Generally, Th17 has been described as an immune response evoked by intranasal immunizations [65]. However, a special immune cell type, the semi-invariant natural killer T (iNKT) cells, can be exploited to deviate the intranasal route from a Th17 response [66]. …”

Section: Protein Conjugate Vaccinesmentioning

confidence: 99%

From Immunologically Archaic to Neoteric Glycovaccines

Cavallari

Libero

2017

Vaccines

View full text Add to dashboard Cite

Polysaccharides (PS) are present in the outermost surface of bacteria and readily come in contact with immune cells. They interact with specific antibodies, which in turn confer protection from infections. Vaccines with PS from pneumococci, meningococci, Haemophilus influenzae type b, and Salmonella typhi may be protective, although with the important constraint of failing to generate permanent immunological memory. This limitation has in part been circumvented by conjugating glycovaccines to proteins that stimulate T helper cells and facilitate the establishment of immunological memory. Currently, protection evoked by conjugated PS vaccines lasts for a few years. The same approach failed with PS from staphylococci, Streptococcus agalactiae, and Klebsiella. All those germs cause severe infections in humans and often develop resistance to antibiotic therapy. Thereby, prevention is of increasing importance to better control outbreaks. As only 23 of more than 90 pneumococcal serotypes and 4 of 13 clinically relevant Neisseria meningitidis serogroups are covered by available vaccines there is still tremendous clinical need for PS vaccines. This review focuses on glycovaccines and the immunological mechanisms for their success or failure. We discuss recent advances that may facilitate generation of high affinity anti-PS antibodies and confer specific immunity and long-lasting protection.

show abstract

NKT Cell Stimulation with α-Galactosylceramide Results in a Block of Th17 Differentiation after Intranasal Immunization in Mice

Cited by 11 publications

References 27 publications

The Frequency of Invariant Natural Killer T Cells Correlates with the Severity of Myocarditis

The Frequency of Invariant Natural Killer T Cells Correlates with the Severity of Myocarditis

Phytosphingosine Derivatives Ameliorate Skin Inflammation by Inhibiting NF-κB and JAK/STAT Signaling in Keratincoytes and Mice

From Immunologically Archaic to Neoteric Glycovaccines

Contact Info

Product

Resources

About