NlCDK1 gene is required for the ovarian development and survival of the brown planthopper, Nilaparvata lugens Stål, as revealed by RNA interference

Hao, Peiying; Ma, Yan; Feng, Yi; Lu, Chaofeng; Yu, Xiaoping

doi:10.1016/j.aspen.2018.01.013

Cited by 4 publications

(6 citation statements)

References 37 publications

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“…Therefore, we think that higher concatenation of schaftoside would suppress more NlCDK1, and resulted in lower survival rate of BPH. Recently, we knockdown the expression of NlCDK1 using RNA interference technique, and found that NlCDK1 was required for the survival of BPH ( Hao et al, 2018 ). However, it is still unclear how the inhibition of NlCDK1 resulted in the death of BPH.…”

Section: Discussionmentioning

confidence: 99%

Schaftoside Interacts With NlCDK1 Protein: A Mechanism of Rice Resistance to Brown Planthopper, Nilaparvata lugens

et al. 2018

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Brown planthopper (BPH) Nilaparvata lugens Stål is a serious insect pest of rice in Asian countries. Active compounds have close relationship with rice resistance against BPH. In this study, HPLC, MS/MS, and NMR techniques were used to identify active compounds in total flavonoids of rice. As a result, a BPH resistance-associated compound, Peak 1 in HPLC chromatogram of rice flavonoids, was isolated and identified as schaftoside. Feeding experiment with artificial diet indicated that schaftoside played its role in a dose dependent manner, under the concentration of 0.10 and 0.15 mg mL-1, schaftoside showed a significant inhibitory effect on BPH survival (p < 0.05), in comparison with the control. The fluorescent spectra showed that schaftoside has a strong ability to bind with NlCDK1, a CDK1 kinase of BPH. The apparent association constant KA for NlCDK1 binding with schaftoside is 6.436 × 103 L/mol. Docking model suggested that binding of schaftoside might affect the activation of NlCDK1 as a protein kinase, mainly through interacting with amino acid residues Glu12, Thr14 and Val17 in the ATP binding element GXGXXGXV (Gly11 to Val18). Western blot using anti-phospho-CDK1 (pThr14) antibody confirmed that schaftoside treatment suppressed the phosphorylation on Thr-14 site of NlCDK1, thus inhibited its activation as a kinase. Therefore, this study revealed the schaftoside-NlCDK1 interaction mode, and unraveled a novel mechanism of rice resistance against BPH.

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Section: Discussionmentioning

confidence: 99%

Schaftoside Interacts With NlCDK1 Protein: A Mechanism of Rice Resistance to Brown Planthopper, Nilaparvata lugens

et al. 2018

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“…Recently, a BPH resistance-associated schaftoside in rice was found to bind to NlCDK1, a CDK1 kinase of BPH involved in multiple biological processes. 49,50 Further study showed that schaftoside suppressed the activation of the NlCDK1, thus the survival of BPH was significant inhibited by the schaftoside. 50 The interaction between schaftoside and NlCDK1 protein is a novel mechanism of rice resistance to BPH.…”

Section: Secondary Metabolites As Active Molecules Associated With Bpmentioning

confidence: 98%

“…Schaftoside, one type of flavonoid, is a group of polyphenolic secondary metabolites in plants. Recently, a BPH resistance‐associated schaftoside in rice was found to bind to NlCDK1, a CDK1 kinase of BPH involved in multiple biological processes . Further study showed that schaftoside suppressed the activation of the NlCDK1, thus the survival of BPH was significant inhibited by the schaftoside .…”

Section: Molecular Players Of Rice Originmentioning

confidence: 99%

“…After being delivered into BPH, the dsRNA binds to the mRNA of target genes in the planthopper in a sequence‐specific manner, resulting in the knockdown of the target genes in planthoppers, which then causes phenotype disturbance of them. By feeding dsRNA using artificial diets or injecting dsRNAs, the most attractive target genes of BPH have been screened, for example when feeding with 0.5 μg μL −1 dsRNA of BPH NlCDK1 , the mRNA level of NlCDK1 in BPH was effectively and significantly reduced . A major challenge for plant‐mediated RNAi‐based BPH control is how to make the dsRNA effectively and economically deliver via transgenic expression in rice into BPH.…”

Section: Plant‐mediated Rna Interference‐based Bph Controlmentioning

confidence: 99%

“…By feeding dsRNA using artificial diets or injecting dsRNAs, the most attractive target genes of BPH have been screened, for example when feeding with 0.5 μg μL −1 dsRNA of BPH NlCDK1, the mRNA level of NlCDK1 in BPH was effectively and significantly reduced. 49 A major challenge for plant-mediated RNAi-based BPH control is how to make the dsRNA effectively and economically deliver via transgenic expression in rice into BPH. Therefore, although a lot of attractive target genes of BPH have been screened, there is only one successful story of plant-mediated RNAi-based BPH control using a planthopper gene in transgenic rice.…”

Section: Plant-mediated Rna Interference-based Bph Controlmentioning

confidence: 99%

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Current understanding of the molecular players involved in resistance to rice planthoppers

Yang

Zhang

2019

Pest Management Science

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Rice planthoppers are the most widespread and destructive pest of rice. Planthopper control depends greatly on the understanding of molecular players involved in resistance to planthoppers. This paper summarizes the recent progress in the understanding of some molecular players involved in resistance to planthoppers and the mechanisms involved. Recent researches showed that host‐plant resistance is the most promising sustainable approach for controlling planthoppers. Planthopper‐resistant varieties with a host‐plant resistance gene have been released for rice products. Integrated planthopper management is a proposed strategy to prolong the durability of host‐plant resistance. Bacillus spp. and their gene products or insect pathogenic fungi have great potential for application in the biological control of planthoppers. Enhancement of the activity of the natural enemies of planthoppers would be more cost‐effective and environmentally friendly. Various molecular processes regulate rice–planthopper interactions. Rice encounters planthopper attacks via transcription factors, secondary metabolites, and signaling networks in which phytohormones have central roles. Maintenance of cell wall integrity and lignification act as physical barriers. Indirect defenses of rice are regulated via chemical elicitors, honeydew‐associated elicitor, amendment with silicon and biochar, and salivary protein of BPH as elicitor or effector. Further research directions on planthopper control and rice defense against planthoppers are also put forward. © 2019 Society of Chemical Industry

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Current understanding of the genomic, genetic, and molecular control of insect resistance in rice

Chen

Guo

et al. 2020

Mol Breeding

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Rice (Oryza sativa) is both a vital source of food and a key model cereal for genomic research. Insect pests are major factors constraining rice production. Here, we provide an overview of recent progress in functional genomics research and the genetic improvements of insect resistance in rice. To date, many insect resistance genes have been identified in rice, and 14 such genes have been cloned via a map-based cloning approach. The proteins encoded by these genes perceive the effectors of insect and activate the defense pathways, including the expression of defense-related genes, including mitogen-activated protein kinase, plant hormone, and transcription factors; and defense mechanism against insects, including callose deposition, trypsin proteinase inhibitors (TryPIs), secondary metabolites, and green leaf volatiles (GLVs). These ongoing functional genomic studies provide insights into the molecular basis of rice-insect interactions and facilitate the development of novel insect-resistant rice varieties, improving long-term control of insect pests in this crucial crop.

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NlCDK1 gene is required for the ovarian development and survival of the brown planthopper, Nilaparvata lugens Stål, as revealed by RNA interference

Cited by 4 publications

References 37 publications

Schaftoside Interacts With NlCDK1 Protein: A Mechanism of Rice Resistance to Brown Planthopper, Nilaparvata lugens

Schaftoside Interacts With NlCDK1 Protein: A Mechanism of Rice Resistance to Brown Planthopper, Nilaparvata lugens

Current understanding of the molecular players involved in resistance to rice planthoppers

Current understanding of the genomic, genetic, and molecular control of insect resistance in rice

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