NLRP3 inflammasome is required for apoptosis of Aggregatibacter actinomycetemcomitans-infected human osteoblastic MG63 cells

Aggregatibacter actinomycetemcomitans, a potent immunoregulator of the periodontal host defense system and alveolar bone homeostasis

“…Taking into consideration clinical studies reporting ultrastructural findings that imply that localized aggressive periodontitis is associated with bacterial invasion of the alveolar bone surface (Carranza et al ., ), it is conceivable that the inflammasome protein, NLRP3, is a critical regulator of A. actinomycetemcomitans induced osteoblast apoptosis (Zhao et al ., ). Zhao et al .…”

“…Zhao et al . () infected isolated human osteoblastic MG63 cell cultures with A. actinomycetemcomitans , which increased cell apoptosis. Aggregatibacter actinomycetemcomitans infection upregulated the expression of inflammatory mediators downstream of the NLRP3 inflammasome protein, and knockdown expression of NLRP3 by small interfering RNA attenuated apoptosis of A. actinomycetemcomitans ‐infected MG63 osteoblastic cells (Zhao et al ., ).…”

“…() infected isolated human osteoblastic MG63 cell cultures with A. actinomycetemcomitans , which increased cell apoptosis. Aggregatibacter actinomycetemcomitans infection upregulated the expression of inflammatory mediators downstream of the NLRP3 inflammasome protein, and knockdown expression of NLRP3 by small interfering RNA attenuated apoptosis of A. actinomycetemcomitans ‐infected MG63 osteoblastic cells (Zhao et al ., ). Although the aforementioned study does not elucidate the role of specific A. actinomycetemcomitans virulence factors, findings from this in vitro investigation highlight that A. actinomycetemcomitans invasion of the alveolar bone surface may directly promote osteoblast apoptosis through the NLRP3 inflammasome (Zhao et al ., ).…”

Aggregatibacter actinomycetemcomitans‐Induced AIM2 Inflammasome Activation Is Suppressed by Xylitol in Differentiated THP‐1 Macrophages

“…Much focus on the correlation between periodontal disease and inflammasomes exists in the current literature, and the authors of this study have also reported the involvement of inflammasome components in chronic periodontitis; however, 17 the role of inflammasomes in AgP remains largely unknown. Limited studies have investigated the relationship between inflammasomes and AgP 18–21 . A. actinomycetemcomitans activates NACHT, LRR and PYD domains‐containing protein 3 (NLRP3) inflammasomes for release of proinflammatory cytokines and apoptosis 18 , 19 and stimulates T helper 17 cell induction and expression of AIM2 and NLRP3 in atherosclerotic lesions that affects progression of atherosclerosis 20 .…”

“…Limited studies have investigated the relationship between inflammasomes and AgP 18–21 . A. actinomycetemcomitans activates NACHT, LRR and PYD domains‐containing protein 3 (NLRP3) inflammasomes for release of proinflammatory cytokines and apoptosis 18 , 19 and stimulates T helper 17 cell induction and expression of AIM2 and NLRP3 in atherosclerotic lesions that affects progression of atherosclerosis 20 . Furthermore, A .…”