NMR-based metabolomic evaluation in dairy cows with displaced abomasum

Basoglu, Abdullah; Başpınar, Nuri; Coşkun, Alparslan

doi:10.3906/vet-1310-52

Cited by 10 publications

(13 citation statements)

References 23 publications

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“…sn-Glycero-3-phosphocoline was lower in liver samples of RDA cows. In our previous study (Basoglu et al 2014), where metabolites identified and quantified by NMR analysis only in plasma samples were valine, 3 b-hydroxybutyrate, alanine, glutamine, glutamate, and succinate. Among these parameters, succinate decreased significantly in cows with RDA.…”

Section: Discussionmentioning

confidence: 90%

Nuclear magnetic resonance (NMR)-based metabolome profile evaluation in dairy cows with and without displaced abomasum

Basoglu

Başpınar

Tenori

et al. 2020

Veterinary Quarterly

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Erdem Gulersoy (2020) Nuclear magnetic resonance (NMR)-based metabolome profile evaluation in dairy cows with and without displaced abomasum, Veterinary Quarterly, 40:1, 1-15, ABSTRACT Background: Displaced abomasum (DA) is a condition of dairy cows that severely impacts animal welfare and causes huge economic losses. Objective: To assess the metabolic status of the disease using metabolomics in serum, urine and liver samples aimed at both water soluble and lipid soluble fractions. Methods: Fifty Holstein multiparous cows with DA (42 left, 8 right) and 20 clinically healthy Holstein multiparous cows were used. Left DA was associated with concomitant ketosis in 19 animals and right in two. NMR-based metabolomics approach and hematological and biochemical analyses were performed. Statistical analysis was carried out on 1 H-NMR data after they have been normalized using PQN method. Results: Contrary to generated PCA score plots the OPLS-supervised method revealed differences between healthy animals and diseased ones based on serum water-soluble samples. While water and lipid soluble metabolites decreased in serum samples, fatty acid fractions and cholesterol were increased in liver samples in DA affected cows. The metabolomic and chemical profiles clearly revealed that cows with DA (especially with LDA) were at risk of ketosis and fatty liver. Serum hippuric acid concentration was significantly higher in healthy cows in comparison with LDA, whereas serum glycine concentration was reported higher for healthy when compared to RDA affected animals. Conclusion: A biochemical network and pathway mapping revealed 'valine, leucine and isoleucine biosynthesis' and 'phenylalanine, tyrosine and tryptophan biosynthesis' as the most probable altered metabolic pathway in DA condition. Serum was advocated as the optimal biological matrix for the 1 H-NMR analysis. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 90%

Nuclear magnetic resonance (NMR)-based metabolome profile evaluation in dairy cows with and without displaced abomasum

Basoglu

Başpınar

Tenori

et al. 2020

Veterinary Quarterly

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“…Because the energy output (milk) exceeds the energy input, every postpartum high-producing dairy cow experiences NEB, but not every cow exhibits LDA [6]. Several previous studies have shown that LDA is accompanied by NEB and lower blood levels of succinate, which is an important biochemical intermediate of the TCA cycle [3,9,39]. In the present study, two important intermediate metabolites of the TCA cycle, citric acid and cis-aconitic acid, were found to be sharply decreased in postpartum dairy cows with LDA, which indicated that postpartum dairy cows with LDA suffer a more severe NEB than healthy postpartum dairy cows.…”

Section: Discussionmentioning

confidence: 99%

“…doi: 10.1292/jvms. been determined through NMR-based metabolomics [3]. Considering the lower sensitivity of the NMR technique, further studies of dairy cows with a displaced abomasum should be performed using other high-throughput analysis technologies.…”

mentioning

confidence: 99%

Identification of disordered metabolic networks in postpartum dairy cows with left displacement of the abomasum through integrated metabolomics and pathway analyses

Guo

Tao

et al. 2020

The Journal of Veterinary Medical Science

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High-producing dairy cows are easily affected by left displacement of the abomasum (LDA) within 4 weeks postpartum. Although LDA is highly associated with metabolic disturbances, the related information on comprehensive metabolic changes, with the exception of some blood biochemical parameters, remains limited. In this study, the changes in plasma metabolites and in the metabolic profile of postpartum dairy cows with LDA were investigated through liquid chromatography coupled with quadrupole time of flight mass spectrometry (LC-Q/ TOF-MS)-based metabolomics, and the metabolic networks related to LDA were constructed through metabolomics pathway analysis (MetPA). An obvious change in the metabolic profile was reflected by significant variations in 68 plasma metabolites in postpartum dairy cows with LDA, and these variations consequently altered 13 metabolic pathways (histidine metabolism, tyrosine metabolism, valine, leucine and isoleucine biosynthesis, phenylalanine, tyrosine and tryptophan biosynthesis, arginine and proline metabolism, tryptophan metabolism, synthesis and degradation of ketone bodies, linoleic acid metabolism, arachidonic acid metabolism, citrate cycle, butanoate metabolism, vitamin B 6 metabolism and pyrimidine metabolism). This study shows that the more detailed information obtained by LC-Q/TOF-MS-based metabolomics and MetPA might contribute to a better understanding of the disordered metabolic networks in postpartum dairy cows with LDA. KEY WORDS: left displacement of the abomasum, liquid chromatography coupled with quadrupole time of flight mass spectrometry, metabolomics, pathway analysis, postpartum dairy cow Left displacement of the abomasum (LDA) is often observed in high-producing dairy cows mainly within 4 weeks postpartum [2, 12, 14]. Specifically, LDA occurs when the abomasum migrates from its normal position to the left lateral abdominal wall, and this migration can cause anorexia and colic in dairy cows and even death in some cases [45]. Dairy cows with LDA exhibit a lower health status, decreased fertility, less milk production and hence a greater culling rate [11, 26]. Many studies have focused on factors related to LDA, and the findings suggest that the species, breed, gender, age, production level, nutrition, metabolism and concurrent diseases likely play an important role in the pathogenesis of LDA [13]. Marked variations in some blood biochemical parameters, such as amyloid A, haptoglobin, non-esterified fatty acids (NEFAs), beta-hydroxy-butyrate (BHBA), Ca, P, Mg, Cl, urea, glucose, aspartate aminotransferase and glutamate dehydrogenase, indicate that the metabolic status of lactating dairy cows with abomasum displacement is strikingly altered [18, 32, 34]. Nevertheless, the available information on comprehensive metabolic changes remains limited, and this topic should be further explored using omics technologies. Metabolomics, as a branch of omics research, aims to identify and quantify small endogenous metabolites in biological samples using a high-throughput analytical p...

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“…In the gastrointestinal tract, histamine levels can be affected by the host allergic or inflammatory responses, altered activity of digestive enzymes, dietary intake, and microbial processes (Smolinska et al 2014). Histamine also can influence the abomasal tone and motility patterns in cows with endotoxemia (Basoglu et al 2014). It has been found that endotoxins and mediators of inflammation such as histamine are released in excessive amounts in cows with acute metritis, mastitis and laminitis, which leads to abomasal atony and distension (Sexton et al 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Nutritional management during the transitional period and negative energy balance in the immediate post parturient period are known to be the most important factors leading to displacement of the abomasum in dairy cows (Radostits et al 2007, Mulligan and Doherty 2008, Doll et al 2009, Basoglu et al 2014. Other reported predisposing factors include genetics, neurological abomasal atony or hypomotility, hypocalcemia, and other metabolic disorders such as ketosis and fatty liver (Holtenius et al 2000,Van Winden andKuiper 2003).…”

Section: Introductionmentioning

confidence: 99%

Changes of hematological and biochemical parameters and levels of pepsinogen, histamine and prostaglandins in dairy cows affected with left displacement of the abomasum

Al-Rawashdeh¹,

Ismail²,

Talafha³

et al. 2017

Polish Journal of Veterinary Sciences

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The aims of this study were to determine the serum levels of pepsinogen, histamine, and prostaglandins F2α and E2 in lactating dairy cows affected with left displacement of the abomasum (LDA). In addition, the hematological and serum biochemical parameters were also determined in cows affected with LDA. A total of 52 adult lactating Holstein-Friesian cows affected with LDA and 30 normal cows (control) were used. In LDA cows, the average age, BCS and body weight were 4.9 ± 1.2 years, 2.5 ± 0.75, and 525 ± 150kg respectively. The average days-in-milk (DIM) in affected cows was 14 ± 6 with a range between 7 to 45 days. There were no significant differences in values of rectal temperature, heart rate and respiration rate between LDA cows and control. Rumen motility was significantly (p≤0.05) decreased in LDA cows. Cows affected with LDA had significantly (p≤0.05) increased glucose levels, and decreased levels of calcium and magnesium. There were significantly (p≤0.05) increased serum levels of pepsinogen and histamine in LDA cows while levels of prostaglandin E2 were significantly decreased in comparison to those in control cows. There were no significant changes in serum levels of prostaglandin F2α. In the hematology analyses, there were no significant changes in cows with LDA when compared to those in control cows. This study provides evidence of a possible role for pepsinogen, histamine and prostaglandin E2 in the etiopathophysiology of LDA in dairy cows.

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NMR-based metabolomic evaluation in dairy cows with displaced abomasum

Cited by 10 publications

References 23 publications

Nuclear magnetic resonance (NMR)-based metabolome profile evaluation in dairy cows with and without displaced abomasum

Nuclear magnetic resonance (NMR)-based metabolome profile evaluation in dairy cows with and without displaced abomasum

Identification of disordered metabolic networks in postpartum dairy cows with left displacement of the abomasum through integrated metabolomics and pathway analyses

Changes of hematological and biochemical parameters and levels of pepsinogen, histamine and prostaglandins in dairy cows affected with left displacement of the abomasum

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