NMR-based metabolomics with enhanced sensitivity

Chandra, Kousik; Al-Harthi, Samah; Sukumaran, Sujeesh; Almulhim, Fatimah; Emwas, Abdul‐Hamid; Atreya, Hanudatta S.; Jaremko, Łukasz; Jaremko, Mariusz

doi:10.1039/d1ra01103k

Cited by 40 publications

(25 citation statements)

References 38 publications

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“…The sensitivity of NMR spectroscopy has been improved significantly by dynamic-nuclear polarization (DNP) ( Ardenkjær-Larsen et al, 2003 ; Emwas et al, 2008 ; Ludwig et al, 2010 ), cryo-probes, ultra-high magnetic fields ( Deborde et al, 2017 ; Emwas et al, 2019 ), and the development of new faster methods. However, sensitivity remains a main limitation in the field ( Emwas et al, 2019 ; Robertson et al, 2020 ; Chandra et al, 2021 ). For instance, secondary metabolites (usually existing at very low concentrations) are beyond the detection limit of NMR spectroscopy, while for volatile molecules can be detected by GC-MS combined with the mass spectrum and retention time ( Emwas et al, 2015 ; Kohlstedt and Wittmann, 2019 ).…”

Section: Advantages and Disadvantages Of Chromatography And Nuclear M...mentioning

confidence: 99%

Fluxomics - New Metabolomics Approaches to Monitor Metabolic Pathways

et al. 2022

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Fluxomics is an innovative -omics research field that measures the rates of all intracellular fluxes in the central metabolism of biological systems. Fluxomics gathers data from multiple different -omics fields, portraying the whole picture of molecular interactions. Recently, fluxomics has become one of the most relevant approaches to investigate metabolic phenotypes. Metabolic flux using 13C-labeled molecules is increasingly used to monitor metabolic pathways, to probe the corresponding gene-RNA and protein-metabolite interaction networks in actual time. Thus, fluxomics reveals the functioning of multi-molecular metabolic pathways and is increasingly applied in biotechnology and pharmacology. Here, we describe the main fluxomics approaches and experimental platforms. Moreover, we summarize recent fluxomic results in different biological systems.

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Section: Advantages and Disadvantages Of Chromatography And Nuclear M...mentioning

confidence: 99%

Fluxomics - New Metabolomics Approaches to Monitor Metabolic Pathways

et al. 2022

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“…Recently, Chandra et al addressed the issues of sensitivity and specificity in in vitro NMR spectroscopy by suggesting new methodological developments [27,28]. Significantly improved sensitivity was reported by combining two previously developed techniques, i.e., (a) controlled detuning of the probe such that the "spin noise tuning optimum" (SNTO) was obtained, and (b) using an externally cylindrical NMR tube made of susceptibility-matched glass, containing a slot-like (non-cylindrical) sample space, instead of a conventional 5 mm NMR tube.…”

Section: In Vitro Vs In Vivo Nmr Spectroscopic Analysismentioning

confidence: 99%

“…In this design, the main horizontal axis of the sample chamber within the sensitive volume of the transmitter/receiver coil, is in parallel with the electric B 1 field of the (saddle) coil. Here, no sample is present in the region most susceptible to the generation of spin noise [27]. The authors also developed a tailored sequence to optimally identify metabolites whose signals are usually hidden under broad lipid and protein signals.…”

Section: In Vitro Vs In Vivo Nmr Spectroscopic Analysismentioning

confidence: 99%

Methodological Developments for Metabolic NMR Spectroscopy from Cultured Cells to Tissue Extracts: Achievements, Progress and Pitfalls

Lutz

Bernard

2022

Molecules

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This is a broad overview and critical review of a particular group of closely related ex vivo and in vitro metabolic NMR spectroscopic methods. The scope of interest comprises studies of cultured cells and excised tissue, either intact or after physicochemical extraction of metabolites. Our detailed discussion includes pitfalls that have led to erroneous statements in the published literature, some of which may cause serious problems in metabolic and biological interpretation of results. To cover a wide range of work from relevant research areas, we consider not only the most recent achievements in the field, but also techniques that proved to be valid and successful in the past, although they may not have generated a very significant number of papers more recently. Thus, this comparative review also aims at providing background information useful for judiciously choosing between the metabolic ex vivo/in vitro NMR methods presented. Finally, the methods of interest are discussed in the context of, and in relation to, other metabolic analysis protocols such as HR-MAS and cell perfusion NMR, as well as the mass spectrometry approach.

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“…A wide range of NMR experiments are available and commonly used in metabolomics applications [42,[56][57][58][59][60][61][62][63] and later on we will focus on NMR results. For instance, onedimensional (1D) experiments are used for detection and quantification of compounds containing atoms such as 1 H, 13 C, 15 N, 31 P [37,64].…”

Section: Metabolomics Analytical Toolsmentioning

confidence: 99%

You Are What You Eat: Application of Metabolomics Approaches to Advance Nutrition Research

Emwas

Al-Rifai

Szczepski

et al. 2021

Foods

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A healthy condition is defined by complex human metabolic pathways that only function properly when fully satisfied by nutritional inputs. Poor nutritional intakes are associated with a number of metabolic diseases, such as diabetes, obesity, atherosclerosis, hypertension, and osteoporosis. In recent years, nutrition science has undergone an extraordinary transformation driven by the development of innovative software and analytical platforms. However, the complexity and variety of the chemical components present in different food types, and the diversity of interactions in the biochemical networks and biological systems, makes nutrition research a complicated field. Metabolomics science is an “-omic”, joining proteomics, transcriptomics, and genomics in affording a global understanding of biological systems. In this review, we present the main metabolomics approaches, and highlight the applications and the potential for metabolomics approaches in advancing nutritional food research.

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NMR-based metabolomics with enhanced sensitivity

Abstract: We combined Spin Noise Tuning Optimum (SNTO) and electric field component-optimized shaped tube to boost sensitivity for NMR-based metabolomics.

Cited by 40 publications

References 38 publications

Fluxomics - New Metabolomics Approaches to Monitor Metabolic Pathways

Fluxomics - New Metabolomics Approaches to Monitor Metabolic Pathways

Methodological Developments for Metabolic NMR Spectroscopy from Cultured Cells to Tissue Extracts: Achievements, Progress and Pitfalls

You Are What You Eat: Application of Metabolomics Approaches to Advance Nutrition Research

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