NMR-based metabonomic study of Chinese medicine Gegen Qinlian Decoction as an effective treatment for type 2 diabetes in rats

Tian, Na; Wang, Junsong; Wang, Pengran; Song, Xingfang; Yang, Ming‐Hua; Kong, Ling–Yi

doi:10.1007/s11306-013-0535-8

Cited by 48 publications

(31 citation statements)

References 58 publications

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“…The liver is a main site of glucose metabolism and is stimulated by insulin to import glucose from the blood and synthesize glycogen, thereby preventing postprandial hyperglycemia4445. Under insulin-resistant conditions, hepatic gluconeogenesis is elevated7, and the key gluconeogenic enzymes G6Pase and PEPCK are significantly increased in the livers of HFD-fed mice46.…”

Section: Discussionmentioning

confidence: 99%

Metabolomics reveals the protective of Dihydromyricetin on glucose homeostasis by enhancing insulin sensitivity

Jiang

Wan

et al. 2016

Sci Rep

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Dihydromyricetin (DMY), an important flavanone found in Ampelopsis grossedentata, possesses antioxidative properties that ameliorate skeletal muscle insulin sensitivity and exert a hepatoprotective effect. However, little is known about the effects of DMY in the context of high-fat diet (HFD)-induced hepatic insulin resistance. Male Sprague-Dawley(SD) rats were fed a HFD(60% fat) supplemented with DMY for 8 weeks. The administration of DMY to the rats with HFD-induced insulin resistance reduces hyperglycemia, plasma levels of insulin, and steatosis in the liver. Furthermore, DMY treatment modulated 24 metabolic pathways, including glucose metabolism, the TCA cycle. DMY significantly enhanced glucose uptake and improved the translocation of glucose transporter 1. The specificity of DMY promoted the phosphorylation of AMP-activated protein kinase (AMPK). In addition, the exposure of HepG2 cells to high glucose concentrations impaired the insulin-stimulated phosphorylation of Akt2 Ser474 and insulin receptor substrate-1 (IRS-1) Ser612, increased GSK-3β phosphorylation, and upregulated G6Pase and PEPCK expression. Collectively, DMY improved glucose-related metabolism while reducing lipid levels in the HFD-fed rats. These data suggest that DMY might be a useful drug for use in type 2 diabetes insulin resistance therapy and for the treatment of hepatic steatosis.

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Section: Discussionmentioning

confidence: 99%

Metabolomics reveals the protective of Dihydromyricetin on glucose homeostasis by enhancing insulin sensitivity

Jiang

Wan

et al. 2016

Sci Rep

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“…These metabolites were the end product of glycolysis, thus their levels elevated in response to an increased glycolytic activity. This is different from the liver tissues with type 2 diabetes [42] . One possible explanation for these results is that HepG2 cell is a kind of tumor cell line with a high rate of aerobic glycolysis, known as the Warburg effect, which is a hallmark of cancer cell glucose metabolism [43] .…”

Section: Discussionmentioning

confidence: 95%

Metabolomic profiling reveals effects of marein on energy metabolism in HepG2 cells

Jiang

Liu

et al. 2017

Preprint

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Previous studies have suggested that Coreopsis tinctoria improves insulin resistance in rats fed with high-fat diet. But little is known about the antidiabetic effects of marein which is the main component of C. tinctoria. This study investigated the effects of ethyl acetate extract of C. tinctoria (AC) on insulin resistance (IR) in rats fed a high-fat diet. High glucose and fat conditions cause a significant increase in blood glucose, insulin, serum TC,TG and LDL-C, leading to an abnormal IR in rats. However, treatment with AC protects against HFD-induced IR by improving fasting serum glucose and lipid homeostasis. High glucose conditions cause a significant decrease in glycogen synthesis and increases PEPCK and G6Pase protein levels and Krebs-cycle-related enzymes levels, leading to an abnormal metabolic state in HepG2 Cells. However, treatment with Marein improves IR by increasing glucose uptake and glycogen synthesis and by downregulating PEPCK and G6Pase protein levels. The statistical analysis of HPLC/MS data demonstrates that Marein restores the normal metabolic state. The results show that AC ameliorates IR in rats and Marein has the potential effect in improving IR by ameliorating glucose metabolic disorders.AbbreviationsACethyl acetate extract of Coreopsis tinctoriaTCATricarboxylic acidHepG2hepatocellular carcinoma cell line2-NBDG2-(N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino)-2-deoxyglucoseG6Paseglucose-6-phosphatasePEPCKphosphoenolpyruvate carboxykinaseIRinsulin resistanceHFDhigh-fat dietSDHAsuccinate dehydrogenase flavoprotein subunitACO2aconitase 2IDH2isocitrate dehydrogenase 2CScitrate synthaseFHfumarate hydrataseMDH2malate dehydrogenaseDLSTdihydrolipoamide S-succinyltransferase

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“…Metabolomics has emerged as a valuable tool to get comprehensive metabolic analysis and discover novel endogenous biomarkers of DM and its complications, which may reveal the pathophysiology of the complex metabolic disorder and aid to evaluate therapeutic strategies. 16,17 Mass spectrometry is a powerful tool for metabolomic analysis with the advantages of high selectivity and sensitivity, coupled with liquid chromatography to make it suitable for throughput metabolic proling. In this study, an ultra-high performance liquid chromatography coupled with quadrupole time-of-ight mass spectrometry (UHPLC-Q-TOF-MS) method integrated with statistical tools was applied to augment the understanding of DN and investigate the therapeutic efficacy of Radix Scutellariae on DN.…”

Section: Introductionmentioning

confidence: 99%

Metabolomics insights into diabetes nephropathy and protective effects of Radix Scutellariae on rats using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

Men

Zhou

et al. 2017

RSC Adv.

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NMR-based metabonomic study of Chinese medicine Gegen Qinlian Decoction as an effective treatment for type 2 diabetes in rats

Cited by 48 publications

References 58 publications

Metabolomics reveals the protective of Dihydromyricetin on glucose homeostasis by enhancing insulin sensitivity

Metabolomics reveals the protective of Dihydromyricetin on glucose homeostasis by enhancing insulin sensitivity

Metabolomic profiling reveals effects of marein on energy metabolism in HepG2 cells

Metabolomics insights into diabetes nephropathy and protective effects of Radix Scutellariae on rats using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

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