NMR metabolic profiling of serum identifies amino acid disturbances in chronic fatigue syndrome

Armstrong, Christopher W.; McGregor, Neil; Sheedy, John R; Buttfield, Ian H; Butt, Henry L.; Gooley, Paul R.

doi:10.1016/j.cca.2012.06.022

Cited by 57 publications

(77 citation statements)

References 37 publications

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“…The anaplerotic amino acids, which serve to maintain TCA cycle capacity (category III), were reduced in female ME/CFS patients. Our findings are in agreement with previous reports showing reductions of Gln, Glu, and Phe in smaller ME/CFS patient cohorts (4,14). Interestingly, a study of ME/CFS patients found changes in urine amino acid concentrations consistent with the specific amino acid pattern we found in serum, and a reduced urinary excretion of the TCA cycle intermediates succinic acid and citric acid was also reported (15).…”

Section: Discussionsupporting

confidence: 93%

“…Compared with many other chronic diseases, patients with ME/CFS have a lower quality of life, with major implications for patients and their families and for society (2,3). Recent research suggests that ME/CFS is associated with changes in fundamental processes of energy metabolism (4,5). Importantly, such metabolic changes may arise from dysregulated physiological response mechanisms that may be relevant in ME/CFS, such as immune activation, inflammation, and receptor-mediated signaling (6,7).…”

Section: Introductionmentioning

confidence: 99%

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Metabolic profiling indicates impaired pyruvate dehydrogenase function in myalgic encephalopathy/chronic fatigue syndrome

et al. 2016

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Metabolic profiling indicates impaired pyruvate dehydrogenase function in myalgic encephalopathy/chronic fatigue syndrome

et al. 2016

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“…2019, 9, x FOR PEER REVIEW 10 of 12 addition, a marked decrease in phenylalanine, alanine, aspartate, and arginine levels was observed in D group. The glycogenic amino acids underwent degradation [29] and reflected an enhancement in the gluconeogenesis pathway that compensates the deficiency of glucose. The results further suggested that carvacrol helps produce energy and amino acid metabolic disorders in P. digitatum.…”

Section: Discussionmentioning

confidence: 99%

The Antifungal Potential of Carvacrol against Penicillium Digitatum through 1H-NMR Based Metabolomics Approach

et al. 2019

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Carvacrol (5-Isopropyl-2-methylphenol), a volatile oil constituent, mainly exists in Labiaceae family plants. Carvacrol has long been studied for its natural antifungal potential and food preservative potential. However, its exact mode of action, especially against Penicillium digitatum (P. digitatum), remains unexplored. Herein, a 1 H-NMR-based metabolomic technique was used to investigate the antifungal mechanism of carvacrol against P. digitatum. The metabolomic profiling data showed that alanine, aspartate, glutamate, and glutathione metabolism were imbalanced in the fungal hyphae. A strong positive correlation was seen between aspartate, glutamate, alanine, and glutamine, with a negative correlation among glutathione and lactate. These metabolic changes revealed that carvacrol-induced oxidative stress had disturbed the energy production and amino acid metabolism of P. digitatum. The current study will improve the understanding of the metabolic changes posed by plant-based fungicides in order to control citrus fruit green mold caused by P. digitatum. Moreover, the study will provide a certain experimental and theoretical basis for the development of novel citrus fruit preservatives.aroused by excessive use of certain routine and synthetic antifungal agents. Over the past few decades, the intentions have been to ensure the hygiene and safety levels of fruit products along with promoting maximum biodegradability. In order to achieve this ambition, researchers are still trying to screen natural products with strong antifungal potential from plants. Moreover, it has also been tried to explore new preservation technologies that can replace synthetic fungicides. Compared with synthetic fungicides, natural antifungal substances have attained much attention in recent years [6][7][8][9].Various essential oils refined from Melaleuca alternifolia have strong inhibitory effects on multiple fungal strains, particularly P. italicum Wehmer and P. digitatum Sacc., and hence can be used as natural preservatives in agricultural and food processing [10]. Previously we have reported that cinnamaldehyde contained in cinnamon has a good antifungal effect against P. italicum and clarifies the method for dynamic detection of the antifungal effect of cinnamaldehyde [11]. In addition, GC-MS and GC-FID techniques successfully separated the essential oils components viz. limonene (87.02%), linalyl acetate (53.76%) and linalool (39.74%) from peels, leaves and flowers of Citrus aurantium var amara, neroli oil appeared as the best fungistatic agent, which reduced the P. digitatum sporulation to 25 % at a 50 mg/mL concentration of oil [12].Carvacrol (5-Isopropyl-2-methylphenol) is the major volatile oil constituent from Labiaceae family plants, such as oregano, mint, and thyme [13]. Carvacrol has many medicinal and edible applications, including antifungal, antimicrobial, anti-inflammatory, insecticidal, antitumor, and other effects [14]. Carvacrol inhibited the growth of P. digitatum and P. italicum in in vitro and in vivo experiments of l...

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“…Confirmation of metabolite assignments were achieved on the 600 MHz spectrometer with the 2-dimensional (2D) 1 H 1 H TOCSY experiment using the phase-sensitive DIPSI2 sequence and 3-9-19 WATERGATE for water suppression with presaturation, and relaxation delay of 1.5 s. Thirty-two scans were employed with m of 50 ms and 4096 T 2 and 1024 T 1 data points. States-TPPI was used for quadrature detection and processed using a QSINE window function, and FID multiplied by 1 Hz line broadening for T 2 data and 0.3 Hz for T 1 data as previously reported (Armstrong et al 2012;Sheedy 2013). 2D spectra were manually phase corrected and automatically baseline corrected in Topspin.…”

Section: Nmr Data Acquisitionmentioning

confidence: 99%

¹H-NMR analysis of the human urinary metabolome in response to an 18-month multi-component exercise program and calcium–vitamin-D₃supplementation in older men

Sheedy

Gooley

Nahid

et al. 2014

Appl. Physiol. Nutr. Metab.

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The musculoskeletal benefits of calcium and vitamin-D3 supplementation and exercise have been extensively studied, but the effect on metabolism remains contentious. Urine samples were analyzed by (1)H-NMR spectroscopy from participants recruited for an 18-month, randomized controlled trial of a multi-component exercise program and calcium and vitamin-D3 fortified milk consumption. It was shown previously that no increase in musculoskeletal composition was observed for participants assigned to the calcium and vitamin-D3 intervention, but exercise resulted in increased bone mineral density, total lean body mass, and muscle strength. Retrospective metabolomics analysis of urine samples from patients involved in this study revealed no distinct changes in the urinary metabolome in response to the calcium and vitamin-D3 intervention, but significant changes followed the exercise intervention, notably a reduction in creatinine and an increase in choline, guanidinoacetate, and hypoxanthine (p < 0.001, fold change > 1.5). These metabolites are intrinsically involved in anaerobic ATP synthesis, intracellular buffering, and methyl-balance regulation. The exercise intervention had a marked effect on the urine metabolome and markers of muscle turnover but none of these metabolites were obvious markers of bone turnover. Measurement of specific urinary exercise biomarkers may provide a basis for monitoring performance and metabolic response to exercise regimes.

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NMR metabolic profiling of serum identifies amino acid disturbances in chronic fatigue syndrome

Cited by 57 publications

References 37 publications

Metabolic profiling indicates impaired pyruvate dehydrogenase function in myalgic encephalopathy/chronic fatigue syndrome

Metabolic profiling indicates impaired pyruvate dehydrogenase function in myalgic encephalopathy/chronic fatigue syndrome

The Antifungal Potential of Carvacrol against Penicillium Digitatum through 1H-NMR Based Metabolomics Approach

¹H-NMR analysis of the human urinary metabolome in response to an 18-month multi-component exercise program and calcium–vitamin-D₃supplementation in older men

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NMR metabolic profiling of serum identifies amino acid disturbances in chronic fatigue syndrome

Cited by 57 publications

References 37 publications

Metabolic profiling indicates impaired pyruvate dehydrogenase function in myalgic encephalopathy/chronic fatigue syndrome

Metabolic profiling indicates impaired pyruvate dehydrogenase function in myalgic encephalopathy/chronic fatigue syndrome

The Antifungal Potential of Carvacrol against Penicillium Digitatum through 1H-NMR Based Metabolomics Approach

1H-NMR analysis of the human urinary metabolome in response to an 18-month multi-component exercise program and calcium–vitamin-D3supplementation in older men

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¹H-NMR analysis of the human urinary metabolome in response to an 18-month multi-component exercise program and calcium–vitamin-D₃supplementation in older men