NMR Metabolomics as an Effective Tool To Unravel the Effect of Light Intensity and Temperature on the Composition of the Marine Microalgae <i>Isochrysis galbana</i>

Aguilera-Sáez, Luis Manuel; Abreu, Ana Cristina; Camacho-Rodríguez, J.; González-López, Cynthia Victoria; Cerón-García, M.C.; Fernández, Ignacio

doi:10.1021/acs.jafc.8b06840

Cited by 33 publications

(23 citation statements)

References 64 publications

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“…For example, the biosynthesis of lipids, pigments, and polyketide metabolites (e.g., biotoxins) in microalgae may be tuned by using operational and abiotic stress factors . We have recently shown the capacity of 1 H NMR metabolomics to successfully monitor metabolic shifts of Isochrysis galbana when varying culture conditions such as temperature and irradiance . To the best of our knowledge, we report herein the first application of an NMR-based metabolomics approach to describe both polar and nonpolar metabolic profiles of A.…”

Section: Introductionmentioning

confidence: 99%

Production of Amphidinols and Other Bioproducts of Interest by the Marine Microalga Amphidinium carterae Unraveled by Nuclear Magnetic Resonance Metabolomics Approach Coupled to Multivariate Data Analysis

Abreu

Molina-Miras

Aguilera-Sáez

et al. 2019

J. Agric. Food Chem.

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This study assessed the feasibility of an NMR metabolomics approach coupled to multivariate data analysis to monitor the naturally present or stresses-elicited metabolites from a long-term (>170 days) culture of the dinoflagellate marine microalgae Amphidinium carterae grown in a fiberglass paddlewheel-driven raceway photobioreactor. Metabolic contents, in particular, in two members of the amphidinol family, amphidinol A and its 7-sulfate derivative amphidinol B (referred as APDs), and other compounds of interest (fatty acids, carotenoids, oxylipins, etc.) were evaluated by altering concentration levels of the f/2 medium nutrients and daily mean irradiance. Operating with a 24 h sinusoidal light cycle allowed a 3-fold increase in APD production, which was also detected by an increase in hemolytic activity of the methanolic extract of A. carterae biomass. The presence of APDs was consistent with the antitumoral activity measured in the methanolic extracts of the biomass. Increased daily irradiance was accompanied by a general decrease in pigments and an increase in SFAs (saturated fatty acids), MUFAs (monounsaturated fatty acids), and DHA (docosahexaenoic acid), while increased nutrient availability lead to an increase in sugar, amino acid, and PUFA ω-3 contents and pigments and a decrease in SFAs and MUFAs. NMR-based metabolomics is shown to be a fast and suitable method to accompany the production of APD and bioactive compounds without the need of tedious isolation methods and bioassays. The two APD compounds were chemically identified by spectroscopic NMR and spectrometric ESI-IT MS (electrospray ionization ion trap mass spectrometry) and ESI-TOF MS (ESI time-of-flight mass spectrometry) methods.

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Section: Introductionmentioning

confidence: 99%

Production of Amphidinols and Other Bioproducts of Interest by the Marine Microalga Amphidinium carterae Unraveled by Nuclear Magnetic Resonance Metabolomics Approach Coupled to Multivariate Data Analysis

Abreu

Molina-Miras

Aguilera-Sáez

et al. 2019

J. Agric. Food Chem.

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“…The proximate cellular concentrations of the primary and secondary metabolites have been enlisted (Table 2). Previous reports have highlighted presence of metabolites like amino acids, organic acids, carbohydrates, alkaloids, alkenones, sterols, α-tocopherol amongst several others (Aguilera-Sáez et al, 2019;Bandarra et al, 2003). In this study, the protein, carbohydrate and total polysaccharide content estimated were 520.02, 80.01 and 168.7 mg g −1 DW of extract respectively.…”

Section: Cellular Metabolite Compositionmentioning

confidence: 49%

Phycoprospecting the nutraceutical potential of Isochrysis sp as a source of aquafeed and other high‐value products

Bhattacharjya

Singh

Mishra

et al. 2021

Aquaculture Research

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Microalgae, besides possessing more efficient photosynthetic machinery than terrestrial plants (Chisti, 2013), have also been recognized as potential source of plethora of novel compounds ranging from proteins to polar and neutral lipids, carotenoids to phenolic compounds and various long-chain polyunsaturated fatty acids amongst others (Marella et al., 2020;De Oliveira et al., 2020). Isochrysis sp., a marine prymnesiophyte, has long been one of the most widely experimented microalgae owing to both its exceptional nutritional value, fast growth rate as well as wide range of tolerance to different environmental cues (Fabregas et al., 1985;Matos et al., 2019). Studies have revealed that since this haptophyte can synthesize range of ω-3 fatty acids like EPA and DHA, vitamins and complex polysaccharides, carotenoids like fucoxanthin, sterols and secondary metabolites, it can serve as an eminent source for mainly nutraceuticals and pharmaceutical sectors (Bandarra et al., 2003;Matos et al., 2017).With a storehouse of metabolites, this microalga has also been explored as an alternative source of natural antioxidant besides its antimicrobial, anti-inflammatory, anti-cancerous and antihypercholesterolaemic properties (Nuño et al., 2013;Sun et al., 2014). Isochrysis, because of its unparalleled nutritional value besides other microalgae like Skeletonema, Chaetoceros, Pavlova, Nannochloropsis, Tetraselmis and Thalassiosira, has been preferred both as a direct natural feed for molluscs, shrimps and as an indirect food source for fish larvae (Dineshbabu et al., 2019). Being crucial for the growth and development rotifers like Brachionus plicatilis and crustaceans like oysters, shellfish and mussels; for rearing of bivalve hatcheries and for conditioning of the brood stock, it plays a pivotal role in aquaculture industries (Zhu et al.,

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“…C16/C18 fatty acids (90.02%), saturated fatty acids (29.82%) and monounsaturated fatty acids (32.67%) were the major constituents of its lipid composition, which is suitable for biodiesel synthesis. In addition, high irradiances stimulated a decrease of chlorophyll a, fucoxanthin and amino acids content and induce the conversion of polar fatty acids to saturated and unsaturated fatty acid in Isochrysis galbana (Aguilera-Sáez et al, 2019).…”

Section: Lightmentioning

confidence: 93%

Influence of different abiotic factors on lipid production by microalgae – a review

Gaignard

Zissis

Buso

2021

OCL

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Research on lipid production in microalgae, especially to target biofuels, has been widely published in recent decades. This literature is mostly focused on the optimization of culture conditions, metabolic engineering, biorefinery and downstream processes. At the lab scale, lipid production is very promising concerning biofuels production based on microalgae due to their high lipid content. However, the accumulation of knowledge associated with the development of processes and technologies for the production of biomass and associated lipids has so far not achieved the hoped-for commercial success due to still too expensive operating costs compared to fossil fuels. This review aims to present the influence of various abiotic factors on the accumulation of lipids and lipid profile in microalgae, such as the depletion of the culture medium, temperature and light, but also most innovative like the induction by hormones. Other optimization procedures and the use of wastewater to improve the cost-viability are discussed in perspective.

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NMR Metabolomics as an Effective Tool To Unravel the Effect of Light Intensity and Temperature on the Composition of the Marine Microalgae Isochrysis galbana

Cited by 33 publications

References 64 publications

Production of Amphidinols and Other Bioproducts of Interest by the Marine Microalga Amphidinium carterae Unraveled by Nuclear Magnetic Resonance Metabolomics Approach Coupled to Multivariate Data Analysis

Production of Amphidinols and Other Bioproducts of Interest by the Marine Microalga Amphidinium carterae Unraveled by Nuclear Magnetic Resonance Metabolomics Approach Coupled to Multivariate Data Analysis

Phycoprospecting the nutraceutical potential of Isochrysis sp as a source of aquafeed and other high‐value products

Influence of different abiotic factors on lipid production by microalgae – a review

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