NMR Study of the Adsorption−Desorption Kinetics of Dissolved Tetraalanine in MCM-41 Mesoporous Material

Pizzanelli, Silvia; Kababya, Shifi; Frydman, Veronica; Landau, Miron V.; Vega, Shimon

doi:10.1021/jp044389e

Cited by 41 publications

(52 citation statements)

References 55 publications

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“…Initial studies of silica biomineralization have focused on single amino acids (alanine, methionine or glycine) [66–70] or up to four residue repeats (dianaline [67] and tetraalanine [68]). As with calcium phosphate biominerals, REDOR measurements ( 15 N– 29 Si, 29 Si– 15 N, and 15 N– 13 C) were used to determine orientation.…”

Section: Studies With Silicatesmentioning

confidence: 99%

Solid-state NMR studies of proteins immobilized on inorganic surfaces

Shaw

2015

Solid State Nuclear Magnetic Resonance

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Solid state NMR is the primary tool for studying the quantitative, site-specific structure, orientation, and dynamics of biomineralization proteins under biologically relevant conditions. Two calcium phosphate proteins, statherin (43 amino acids) and leucine rich amelogenin protein (LRAP; 59 amino acids), have been studied in depth and have different dynamic properties and 2D- and 3D-structural features. These differences make it difficult to extract design principles used in nature for building materials with properties such as high strength, unusual morphologies, or uncommon phases. Consequently, design principles needed for developing synthetic materials controlled by proteins are not clear. Many biomineralization proteins are much larger than statherin and LRAP, necessitating the study of larger biomineralization proteins. More recent studies of the significantly larger full-length amelogenin (180 residues) represent a significant step forward to ultimately investigate the full diversity of biomineralization proteins. Interactions of amino acids, a silaffin derived peptide, and the model LK peptide with silica are also being studied, along with qualitative studies of the organic matrices interacting with calcium carbonate. Dipolar recoupling techniques have formed the core of the quantitative studies, yet the need for isolated spin pairs makes this approach costly and time intensive. The use of multidimensional techniques to study biomineralization proteins is becoming more common, methodology which, despite its challenges with these difficult-to-study proteins, will continue to drive future advancements in this area.

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Section: Studies With Silicatesmentioning

confidence: 99%

Solid-state NMR studies of proteins immobilized on inorganic surfaces

Shaw

2015

Solid State Nuclear Magnetic Resonance

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“…The D-alanine is more tightly bound to the mesoporous composite SBA-15 than its L-isomer. Vega and coworkers used 2 H MAS NMR to determine the adsorption and desorption rates of a deuterium-labeled tetraalanine salt at the surface of the pores of MCM-41 [191]. The dynamic deuterium MAS NMR approach can also be applied to many other molecular adsorption-desorption systems, as long as the signal-to-noise ratio is sufficient.…”

Section: Immobilization Of Small Biomolecules On Mesoporous Materialsmentioning

confidence: 99%

Immobilization of Biomolecules on Mesoporous Structured Materials

Vinu

Gokulakrishnan

Mori

et al. 2007

Bio‐inorganic Hybrid Nanomaterials

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Various functional systems have been developed using artificially constructed structures and synthesized compounds, and the nanotechnology based on them has recently received much attention. However, current nanoscale technologies are still highly inferior to those seen in natural systems. The efficiency of the energy conversion that occurs in mitochondrial and photosynthetic systems far exceeds that obtained in artificial systems. A dog can smell and a bat can hear far more sensitively than most artificial sensors. The information processing exhibited by the brain and nervous systems is much more sophisticated than that exhibited by current computers. Nature developed superior nanotechnologies to our own several billion years ago. Therefore, the use of biomaterials for the development of various functional systems can be a very practical approach to reaching several goals in current nanotechnologies.Unfortunately, biomolecules in most cases give their best performance under ambient conditions and are not mechanically and thermally strong enough to be used in harsh conditions. In order to overcome such difficulties, hybridization of biomolecules with supporting materials such as lipid assemblies, polymers and inorganic materials has been proposed to attain both biological functions and mechanical stability . Biomolecules are frequently immobilized on silica supports through a sol-gel process. As advanced silica structures, mesoporous silica molecular sieves and related materials have received much attention as inorganic supports for biomolecules [31][32][33][34][35][36][37][38][39][40]. Mesoporous silica materials are chemically and mechanically stable and resistant to microbial attack. In addition, chemical modification to introduce organic functional groups to the inner silica pore wall is highly possible. Outstanding properties of the mesoporous structure also lie in their high specific surface area and huge specific pore volume, which are definitely advantageous for the efficient accommodation of biomolecules. In addition, the narrow pore size distribution could provide reliable immobilization of biomolecules. According to the j113 Bio-inorganic Hybrid Nanomaterials. Edited

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“…In a 2 H MAS NMR study of tetraalanine in MCM‐41 by Pizzanelli et al. ,103 the adsorption‐desorption kinetics at the surface‐liquid interface could be probed as a function of water content and temperature. The adsorption and desorption rates, as well as the activation energies, were determined by sideband analysis of the temperature‐dependent 2 H MAS NMR measurements.…”

Section: Amino Acids and Peptides In Confinement For Medical Applicmentioning

confidence: 99%

Recent Advances in Solid State NMR of Small Molecules in Confinement

Werner

Rothermel

Breitzke

et al. 2014

Israel Journal of Chemistry

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Dedicated to Professor Shimon Vega on the Occasion of His 70th Birthday duced molecules in the confined state. Solid state (ss) NMR gives a unique insight into the dynamics, the guesthost interactions, and the binding sites of porous materials and is probably the most powerful characterization method for probing a huge variety of real-life systems. Recent results in research of microporous zeolites and periodically mesoporous silica (PMS) materials using NMR will be highlighted.

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NMR Study of the Adsorption−Desorption Kinetics of Dissolved Tetraalanine in MCM-41 Mesoporous Material

Cited by 41 publications

References 55 publications

Solid-state NMR studies of proteins immobilized on inorganic surfaces

Solid-state NMR studies of proteins immobilized on inorganic surfaces

Immobilization of Biomolecules on Mesoporous Structured Materials

Recent Advances in Solid State NMR of Small Molecules in Confinement

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