NMR Study on the Interaction of Trehalose with Lactose and Its Effect on the Hydrogen Bond Interaction in Lactose

Abstract

Trehalose, a well-known stress-protector of biomolecules, has been investigated for its effect on the mobility, hydration and hydrogen bond interaction of lactose using diffusion-ordered NMR spectroscopy and NMR of hydroxy protons. In ternary mixtures of trehalose, lactose and water, the two sugars have the same rate of diffusion. The chemical shifts, temperature coefficients, vicinal coupling constants and ROE of the hydroxy protons in trehalose, lactose and sucrose were measured for the disaccharides alone in water/acetone-d 6 solutions as well as in mixtures. The data indicated that addition of trehalose did not change significantly the strength of the hydrogen bond interaction between GlcOH3 and GalO5' in lactose. Small upfield shifts were however measured for all hydroxy protons when the sugar concentration was increased. The chemical shift of the GlcOH3 signal in lactose showed less change, attributed to the spatial proximity to GalO5'. Chemical exchange between hydroxy protons of lactose and trehalose was observed in the ROESY NMR spectra. Similar effects were observed with sucrose indicating no specific effect of trehalose at the concentrations investigated (73 to 763 mg/mL) and suggesting that it is the concentration of hydroxy groups more than the type of sugars which is guiding intermolecular interactions.

A Distinct Proof on Interplay between Trehalose and Guanidinium Chloride for the Stability of Stem Bromelain

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Sweet Confinement: Glucose and Carbohydrate Osmolytes in Reverse Micelles

“…We recently reported on this phenomenon and showed that the chemical exchange within the RM interior is at least 20–100× slower in the RM interior than in the bulk aqueous solution. Broadening and coalescence as a result of chemical exchange are observed in the 1 H NMR spectra of the other osmolytes we studied. − Many NMR studies simplify the 1 H NMR spectrum of aqueous glucose solutions by using D 2 O to eliminate the dominant water peak from the spectra, ,− while also displacing the glucose OH protons and deuterons. By using H 2 O in our experiments, we were able to observe the appearance and narrowing of these signals in AOT RMs.…”

How do interfaces alter the dynamics of supercooled water?

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