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Valcheva, Eva; Veleva, S.; Valchev, Ivo; Димитров, Иван

doi:10.1023/a:1010310706612

Cited by 6 publications

(6 citation statements)

References 4 publications

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“…Therefore in this analysis, the enzyme kinetics study utilizing xylanase (the key xylan backbone degrading enzyme) was analyzed based on the modified Prout-Tompkins formula for the processing of simple reducing sugars. Similar findings for the xylanase activity on kraft pulp have also been documented (Valcheva et al, 2000).…”

Section: Resultssupporting

confidence: 85%

“…The detailed calculation for each temperature is shown in the supplementary data. Significantly higher Rs max was obtained from the study compared to the one achieved by Valcheva et al (2000) where only 37.5 mg/g of sugar was obtained from kraft pulp xylanase hydrolysis. The high yield of the Rs max achieved in the study showed the high efficiency of xylanase hydrolysis on the pretreated kenaf stem.…”

Section: Temperature Dependence Of the Hydrolysis Processmentioning

confidence: 56%

“…The linear relationship is significant for the calculations of the kinetic constant of χ and k (Table 2). Valcheva et al (2000) had also performed a similar study with four different temperatures (20 to 50°C) on kraft pulp and all of them showed a straight line with high correlation coefficient. In this analysis, the correlation coefficient, R, and standard deviation calculated by the Revised Prout-Tompkins equation is 0.9097 and 0.119, respectively.…”

Section: Temperature Dependence Of the Hydrolysis Processmentioning

confidence: 93%

“…The thermal inactivation of the enzyme may contribute to the decrease of the reducing sugars at 50°C at a longer hydrolysis time. (Valcheva et al, 2000).…”

Section: Temperature Dependence Of the Hydrolysis Processmentioning

confidence: 99%

“…The explanation on the kinetic process needs to consider most heterogeneous equations relating to other activity of heterogeneous catalytic, topochemical and diffusion (Valcheva et al, 2000), and most heterogeneous conditions kinetic research applied the first method of hydrolysis velocity as the parameter of the primary concentration of enzymes (Chrastil, 1988;Nill et al, 2018). Due to its significant correlation variable (R 2 = 0.9974), the revised Prout-Tompkins equation, notably, best described the heterogeneous enzyme hydrolysis kinetics (Valtcheva et al, 2003) Towards this end, this study aims to understand the hydrolysis mechanism on the pretreated kenaf by xylanase (the main hemicellulosic xylan degrading enzyme) by a systematic kinetic study into simple reducing sugars.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Enzyme Kinetics Study for Heterogeneous System of Pretreated Kenaf Hydrolysis

Azelee¹,

Nordin²,

Illias³

et al. 2020

JST

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The peculiarity of spatially restricted diffusion and molecular collision processes results in considerable contrast in a reaction between the reactant and catalyst in the heterogeneous system from its corresponding homogeneous structure. The identification of the enzymatic hydrolysis process of pre-treated kenaf and to convert it into simple sugars employing a systematic kinetic investigation is the aims of this study. The influence of substrate concentration on xylanase hydrolysis was performed in water bath shakers. In-house recombinant xylanase expressed in Pichia pastoris was used for the hydrolysis at pH 4.0 in 50 mM sodium citrate buffer with 200 rpm agitation. Modified Prout-Tompkins equation was used for the heterogeneous substrate hydrolysis. The results obtained show that temperature simultaneously influenced the time dependency of the reducing sugar yield. Dependence of the enzymatic rate of reaction can be calculated effectively on the conversion of substrates over different temperatures. The activation energy needed for pretreated kenaf hydrolysis was among the least compared to other lignocelluloses, which was only 25.15 kJ/mol. In conclusion, the exponential kinetic equation by the Modified Prout-Tompkins equation offers a solid understanding of xylanase hydrolysis on the pretreated kenaf. Thus, the prediction of the degree of hydrolysis required at the predetermined temperature and time values used can be quickly and precisely determined.

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Section: Resultssupporting

confidence: 85%

Section: Temperature Dependence Of the Hydrolysis Processmentioning

confidence: 56%

Section: Temperature Dependence Of the Hydrolysis Processmentioning

confidence: 93%

“…The thermal inactivation of the enzyme may contribute to the decrease of the reducing sugars at 50°C at a longer hydrolysis time. (Valcheva et al, 2000).…”

Section: Temperature Dependence Of the Hydrolysis Processmentioning

confidence: 99%