No association between MTHFR gene C677T/A1298C polymorphisms, serum folate, vitamin B12, homocysteine levels, and prostate cancer in an Algerian population

Abstract

BackgroundMethylenetetrahydrofolate reductase (MTHFR) is an important enzyme involved in folate and homocysteine metabolism, which are necessary for DNA methylation and nucleotide synthesis. Genetic polymorphisms that reduce MTHFR activity have been linked to several diseases, including prostate cancer. In this study, we aimed to investigate whether MTHFR polymorphisms, along with serum levels of folate, vitamin B12, and homocysteine, are associated with prostate cancer risk in the Algerian population.MethodsA total of 106 Algerian men with newly diagnosed prostate cancer and 125 healthy controls were included in this case‐control study. The MTHFR C677T and A1298C polymorphisms were analyzed using PCR/RFLP and Real‐Time PCR TaqMan® assays, respectively. Serum levels of folate, total homocysteine, and vitamin B12 were measured using an automatic biochemistry analyzer.ResultsWe found no significant differences in the genotype frequency of A1298C and C677T between prostate cancer patients and controls. Moreover, serum levels of folate, total homocysteine, and vitamin B12 were not significantly associated with prostate cancer risk (p > 0.05). However, age and family history were identified as significant risk factors (OR = 1.178, p = 0.00 and OR = 10.03, p = 0.007, respectively).ConclusionOur study suggests that MTHFR C677T and A1298C, as well as serum levels of folate, total homocysteine, and vitamin B12, are not associated with prostate cancer risk in the Algerian population. However, age and family history are significant risk factors. Further studies with a larger sample size are required to confirm these findings.

Associations between MTHFR gene polymorphisms (C677T and A1298C) and genetic susceptibility to prostate cancer: a systematic review and meta-analysis

“…Frontiers in Genetics frontiersin.org Frontiers in Genetics frontiersin.org Collin et al, 2009;Muslumanoglu et al, 2009;Cai et al, 2010;Safarinejad et al, 2010;Wu et al, 2010;Kosova et al, 2011;Küçükhüseyin et al, 2011;Fard-Esfahani et al, 2012;Kobayashi et al, 2012;Mandal et al, 2012;Vidal et al, 2012;de Vogel et al, 2013;López-Cortés et al, 2013;Ghasemi et al, 2014;Wu et al, 2016;TELLOUCHE-BOUHOUHOU et al, 2018;Mouhoub-Terrab et al, 2023) included 12,455 cases and 13,900 controls with the C677T polymorphism and 6,396 cases and 8,913 controls with the A1298C polymorphism (Table 1). The participants included Caucasians, Asians, and individuals of mixed races.…”

Nutrigenomics and Integrative Medicine: Shaping the Future of Cancer Management

“…In vitro and preclinical investigations demonstrate that plant-based dietary components effectively regulate detoxification enzymes and activate essential signalling pathways. Additionally, specific dietary elements, such as flavonoids and vitamins B12, E, and C, are known for their ability to repair oxidative DNA damage [45][46][47][48][49].…”