No carry over of unmetabolised deoxynivalenol in milk of dairy cows fed high concentrate proportions

Keese, Christina; Meyer, Ulrich; Valenta, Hana; Schollenberger, Margit; Starke, A.; Weber, I.; Rehage, J.; Breves, Gerhard; Dänicke, Sven

doi:10.1002/mnfr.200800077

Cited by 38 publications

(35 citation statements)

References 36 publications

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“…The concentration of DON and DOM-1 in serum was measured by liquid chromatography-tandem mass spectrometry after extraction with ethyl acetate on a ChemElut cartridge and clean up by IACs (Keese et al, 2008). The detection limit was 0.2 ng/mL.…”

Section: Serum Don and Dom-1 Analysismentioning

confidence: 99%

Effects of deoxynivalenol in naturally contaminated wheat on feed intake and health status of horses

et al. 2015

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The present study examined the short-term effects of deoxynivalenol (DON), administered at two different concentrations via a feed preparation using naturally contaminated wheat, on feed intake, liver and kidney metabolism and immunomodulatory properties in horses. Twelve geldings were randomly assigned to one of three dietary treatments for 21 days. DON was provided via naturally contaminated wheat (14.6 ± 6.5 mg DON/kg dry matter). The daily feed intake was adjusted to 4 kg of wheat and 1.7 kg of silage per 100 kg of body weight (BW). Horses were fed one of the following diets: control wheat with 0% contaminated wheat (CON), wheat mixture containing 53 ± 2% of DON-contaminated wheat [low DON intake (LDI)] or wheat mixture containing 78 ± 4% of DON-contaminated wheat [high DON intake (HDI)]. CON, LDI and HDI corresponded to a targeted daily DON intake via the complete ration of <5, 50 and 75 μg/kg BW, respectively. None of the horses demonstrated any clinical signs commonly associated with the intake of DON such as colic or depression. HDI was associated with lower daily wheat intake on day 21. Serum DON concentrations increased with higher DON intake. The non-toxic DON metabolite, deepoxy-deoxynivalenol (DOM-1) was only detected on day 21 of the DON feeding period. No changes in haematological and serum parameters or serum globulins or in the ex vivo proliferation response of peripheral blood mononuclear cells were observed. These results suggest that horses are less sensitive to DON exposure than other domestic species, for example, swine. Therefore, the European Commission guidance value for critical DON concentrations in swine feed (complete diet) of 0.9 mg/kg could be safely applied for rations intended for feeding adult horses as well.

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Section: Serum Don and Dom-1 Analysismentioning

confidence: 99%

Effects of deoxynivalenol in naturally contaminated wheat on feed intake and health status of horses

et al. 2015

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“…To determine the effects of DON on granulosa cell steroidogenesis, cells were treated from day 2 with 0, 1, 10, or 100 ng/ml DON (in methanol) or vehicle (methanol alone), and cells and media were recovered on day 6; these doses were based on concentrations of 2-14 ng/ml DON reported in serum of cattle fed a contaminated concentrate (Keese et al 2008). Apoptosis was measured using an Annexin V-FITC apoptosis detection kit (Sigma-Aldrich) after treating cells with an effective dose of DON for 4 days.…”

Section: Experimental Treatmentsmentioning

confidence: 99%

Effects of the mycotoxin deoxynivalenol on steroidogenesis and apoptosis in granulosa cells

Guerrero-Netro¹,

Chorfi²,

Price³

2015

Reproduction

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Mycotoxins can reduce fertility and development in livestock, notably in pigs and poultry, although the effect of most mycotoxins on reproductive function in cattle has not been established. One major mycotoxin, deoxynivalenol (DON), not only targets immune cells and activates the ribotoxic stress response (RSR) involving MAPK activation, but also inhibits oocyte maturation in pigs. In this study, we determined the effect of DON on bovine granulosa cell function using a serum-free culture system. Addition of DON inhibited estradiol and progesterone secretion, and reduced levels of mRNA encoding estrogenic (CYP19A1) but not progestogenic (CYP11A1 and STAR) proteins. Cell apoptosis was increased by DON, which also increased FASLG mRNA levels. The mechanism of action of DON was assessed by western blotting and PCR experiments. Addition of DON rapidly and transiently increased phosphorylation of MAPK3/1, and resulted in a more prolonged phosphorylation of MAPK14 (p38) and MAPK8 (JNK). Activation of these pathways by DON resulted in time-and dose-dependent increases in abundance of mRNA encoding the transcription factors FOS, FOSL1, EGR1, and EGR3. We conclude that DON is deleterious to granulosa cell function and acts through a RSR pathway.

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“…DON is mostly biotransformed within the rumen and detected in milk as DON and deepoxy-deoxynivalenol (DOM-1) [20][21][22], whereas T-2 has been detected in the milk without metabolization [23] and metabolized into HT-2, NEO, 4-deacetylneosolaniol, and 4 more unknown metabolites [24]. Metabolites could have different toxic effects than the parent mycotoxin [25,26].…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a high performance liquid chromatographic–mass spectrometry method for the simultaneous quantification of 10 trichothecenes in ultra-high temperature processed cow milk

Flores-Flores

González-Peñas

2015

Journal of Chromatography A

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No carry over of unmetabolised deoxynivalenol in milk of dairy cows fed high concentrate proportions

Cited by 38 publications

References 36 publications

Effects of deoxynivalenol in naturally contaminated wheat on feed intake and health status of horses

Effects of deoxynivalenol in naturally contaminated wheat on feed intake and health status of horses

Effects of the mycotoxin deoxynivalenol on steroidogenesis and apoptosis in granulosa cells

Development and validation of a high performance liquid chromatographic–mass spectrometry method for the simultaneous quantification of 10 trichothecenes in ultra-high temperature processed cow milk

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