No differences in support of thrombin generation by neonatal or adult platelets

Bernhard, Heike; Rosenkranz, A.; Novak, M. John; Leschnik, B.; Petritsch, M.; Rehak, Thomas; Köfeler, Harald; Ulrich, Dietmar; Muntean, W.

doi:10.1055/s-0037-1621492

Cited by 8 publications

(6 citation statements)

References 13 publications

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“…

Phosphatidylserine (PS) exposure: Annexin V, which binds PS with high affinity, can be used to characterize the procoagulant phenotype of activated platelets. Analysis of PS exposure through measurement of Annexin V binding via flow cytometry has shown that the procoagulant phenotype is similar for both cord blood-derived platelets and adult platelets following stimulation with thrombin (17,18). Along these lines, phospholipid content of adult and cord blood platelets has also been reported to be similar (17,18).

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Section: Introductionmentioning

confidence: 99%

“…Analysis of PS exposure through measurement of Annexin V binding via flow cytometry has shown that the procoagulant phenotype is similar for both cord blood-derived platelets and adult platelets following stimulation with thrombin (17,18). Along these lines, phospholipid content of adult and cord blood platelets has also been reported to be similar (17,18). The procoagulant phenotype of peripheral blood-derived neonatal platelets has yet to be defined.…”

Section: Introductionmentioning

confidence: 99%

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Neonatal platelets: mediators of primary hemostasis in the developing hemostatic system

2014

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The human hemostatic system is developmentally regulated, resulting in qualitative and quantitative differences in the mediators of primary and secondary hemostasis as well as fibrinolysis in neonates and infants. Although gestational and age related differences in coagulation factor levels occur, the existence of a unique neonatal platelet phenotype remains controversial. Complicated by difficulties in obtaining adequate neonatal blood volumes with which to perform functional assays, ambiguity surrounds the characterization of neonatal platelets. Thus, much of the current knowledge of neonatal platelet function has been based on studies from cord blood samples. Studies suggest that cord blood-derived platelets, as a surrogate for neonatal platelets, are hypo-functional when compared to adult platelets. This relative platelet dysfunction combined with a propensity toward thrombocytopenia in the Neonatal Intensive Care Unit population, creates a clinical conundrum regarding the appropriate administration of platelet transfusions. This review provides an appraisal of the distinct functional phenotype of neonatal platelets. Neonatal platelet transfusion practices and the impact of the relatively hypo-functional neonatal platelet on those practices will be considered.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Neonatal platelets: mediators of primary hemostasis in the developing hemostatic system

2014

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“…Regarding the venoms used in this study and their dependence on endogenous phospholipids for their coagulotoxicity, our previous work showed that D. russelii venom is unusually highly dependent upon phospholipid for the activation of Factor X [ 49 ], which is not mirrored by species within the genera Macrovipera , Oxyuranus , or Pseudonaja [ 49 , 67 ]. However, no differences in phospholipid levels have been observed between paediatric and adult plasma [ 68 ], suggesting that phospholipid levels do not explain the differential potency of D. russelii venom upon the two plasmas. The observed difference may therefore be related to the mechanism of procoagulant action and requires future work to elucidate.…”

Section: Resultsmentioning

confidence: 99%

Children and Snakebite: Snake Venom Effects on Adult and Paediatric Plasma

Zdenek

Rodrigues

Bourke

et al. 2023

Toxins

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Snakebite is a globally neglected tropical disease, with coagulation disturbances being the primary pathology of many deadly snake venoms. Age-related differences in human plasma have been abundantly reported, yet the effect that these differences pose regarding snakebite is largely unknown. We tested for differences in coagulotoxic effects (via clotting time) of multiple snake venoms upon healthy human adult (18+) and paediatric (median 3.3 years old) plasma in vivo and compared these effects to the time it takes the plasmas to clot without the addition of venom (the spontaneous clotting time). We tested venoms from 15 medically significant snake species (from 13 genera) from around the world with various mechanisms of coagulotoxic actions, across the three broad categories of procoagulant, pseudo-procoagulant, and anticoagulant, to identify any differences between the two plasmas in their relative pathophysiological vulnerability to snakebite. One procoagulant venom (Daboia russelii, Russell’s Viper) produced significantly greater potency on paediatric plasma compared with adult plasma. In contrast, the two anticoagulant venoms (Pseudechis australis, Mulga Snake; and Bitis cornuta, Many-horned Adder) were significantly more potent on adult plasma. All other procoagulant venoms and all pseudo-procoagulant venoms displayed similar potency across both plasmas. Our preliminary results may inform future studies on the effect of snake venoms upon plasmas from different age demographics and hope to reduce the burden of snakebite upon society.

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“…The phospholipid content and baseline exposure of platelet surface phosphatidylserine is comparable in adults and newborns [65,66]. However, more platelet microparticles are generated and more phosphatidylserine molecules are exposed in the term and preterm platelets when thrombin or calcium ionophores were used as activators [67].…”

Section: Newborn Plateletsmentioning

confidence: 99%

Platelets in the Newborn

Esiaba¹,

Mousselli²,

Faison³

et al. 2019

Neonatal Medicine

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Platelets were first described in the mid-nineteenth century. Since then, their roles were identified in hemostasis and thrombosis, inflammation, leukocyte interactions, angiogenesis, and cancer growth. But there is little information about such platelet functions in the newborn. Several studies highlighted some platelet differences between newborns and adults. Yet, in spite of these differences, healthy newborns appear to be adequately protected. A number of factors, however, were reported to negatively affect neonatal platelets. These include maternal hypertensive disorders or infections, neonatal asphyxia or respiratory distress, therapies such as ampicillin or indomethacin, and treatment modalities such as ventilators, nitric oxide, or extracorporeal membrane oxygenation (ECMO). Their effects on newborn platelets are usually transitory, lasting from several hours to a few days or weeks. If these effects are well characterized, they could serve as reporters for diagnosis and monitoring during therapy. Careful studies of neonatal platelets are needed to improve the understanding of basic physiology and pathophysiology in this cohort and to identify possible targets for intervention and therapy.

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No differences in support of thrombin generation by neonatal or adult platelets

Abstract: Our results do not provide any evidence that a different PL content or expression of neonatal platelets may alter TG in neonates.

Cited by 8 publications

References 13 publications

Neonatal platelets: mediators of primary hemostasis in the developing hemostatic system

Neonatal platelets: mediators of primary hemostasis in the developing hemostatic system

Children and Snakebite: Snake Venom Effects on Adult and Paediatric Plasma

Platelets in the Newborn

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