No evidence for a bovine mastitis<i>Escherichia coli</i>pathotype

Leimbach, Andreas; Vollmers, John; Daniel, Rolf; Dobrindt, Ulrich

doi:10.1101/096479

Cited by 13 publications

(20 citation statements)

References 159 publications

(441 reference statements)

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“…Several studies have examined E. coli isolates for evidence of a specific subset of mammary-pathogenic E. coli (MPEC) with sometimes conflicting results. A variety of E. coli phylogroups have been associated with IMI and in some studies particular phylogroups or phylogenetic lineages were more commonly associated with mastitis, whereas in others no association was found (Blum et al, 2008;Blum and Leitner, 2013;Keane, 2016;Leimbach et al, 2017). However, limitations of the strain-typing methods used may have contributed to the failure to detect MPEC as a recent whole-genome comparison of 66 phylogroup A mammary-derived E. coli with phylogroup A dairy farm-derived E. coli found that mammary isolates had a reduction in phylogenetic diversity and a larger core genome but smaller pangenome than dairy farm isolates (Goldstone et al, 2016).…”

Section: E Colimentioning

confidence: 99%

Symposium review: Intramammary infections—Major pathogens and strain-associated complexity

Keane

2019

Journal of Dairy Science

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Intramammary infection (IMI) is one of the most costly diseases to the dairy industry. It is primarily due to bacterial infection and the major intramammary pathogens include Escherichia coli, Streptococcus uberis, and Staphylococcus aureus. The severity and outcome of IMI is dependent on several host factors including innate host resistance, energy balance, immune status, parity, and stage of lactation. Additionally, the infecting organism can influence the host immune response and progression of disease. It is increasingly recognized that not only the infecting pathogen species, but also the strain, can affect the transmission, severity, and outcome of IMI. For each of 3 major IMI-associated pathogens, S. aureus, Strep. uberis, and E. coli, specific strains have been identified that are adapted to the intramammary environment. Strain-dependent variation in the host immune response to infection has also been reported. The diversity of strains associated with IMI must be considered if vaccines effective against the full repertoire of mammary pathogenic strains are to be developed. Although important advances have been made recently in understanding the molecular mechanism underpinning strain-specific virulence, further research is required to fully elucidate the cellular and molecular pathogenesis of mammary adapted strains and the role of the strain in influencing the pathophysiology of infection. Improved understanding of molecular pathogenesis of strains associated with bovine IMI will contribute to the development of new control strategies, therapies, and vaccines. The development of enabling technologies such as pathogenomics, transcriptomics, and proteomics can facilitate system-level studies of strain-specific molecular pathogenesis and the identification of key mediators of host-pathogen interactions.

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Section: E Colimentioning

confidence: 99%

Symposium review: Intramammary infections—Major pathogens and strain-associated complexity

Keane

2019

Journal of Dairy Science

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“…De acuerdo con esto último, estudios más recientes a nivel genómico han revelado la presencia de grupos de genes que codifican para sistemas se secreción tipo VI, biosíntesis de lipopolisacáridos, formación de biopelículas y sistemas de captación de hierro, característicos de las E. coli asociadas a mastitis bovina (5)(6)(7)(8) . Sin embargo, otros autores sugieren que no hay evidencia suficiente de que las E. coli causantes de mastitis pertenecen a un patotipo particular (9) . Diversos análisis filogenéticos de E. coli han permitido clasificarlas en cuatro grupos principales A, B1, B2 y D, en donde las bacterias comensales no patógenas pertenecen principalmente a los grupos A y B1, aunque estos filogrupos también incluyen patógenos importantes.…”

Section: Introductionunclassified

“…Diversos análisis filogenéticos de E. coli han permitido clasificarlas en cuatro grupos principales A, B1, B2 y D, en donde las bacterias comensales no patógenas pertenecen principalmente a los grupos A y B1, aunque estos filogrupos también incluyen patógenos importantes. Mientras al grupo B2 y en menor proporción al D, pertenecen las bacterias asociadas con infecciones extra-intestinales (9,10) . Para el caso de E. coli causantes de mastitis bovina se ha observado que pertenecen principalmente a los grupos filogenéticos A y B1 (11,12,13) .…”

Section: Introductionunclassified

“…En este sentido se ha reportado que las E. coli asociadas a mastitis bovina tanto persistente como transitoria, muestran diversidad fenotípica y genotípica (14) . Asimismo, el análisis a nivel genómico de diversos aislados de E. coli, causantes de mastitis bovina y comensales, ha mostrado en ninguno de los casos las bacterias pueden clasificarse en un grupo filogenético en particular, sino que al parecer en ambos casos se han originado de diferentes linajes (9) .…”

Section: Introductionunclassified

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Relación genética, formación de biopelículas, movilidad y virulencia de Escherichia coli aislada de mastitis bovina

Cruz-Soto

Toro-Castillo

Munguía-Magdaleno

et al. 2020

Rev. Mex. Cienc. Pecu.

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Escherichia coli es una de las bacterias ambientales que frecuentemente se ha visto implicada en infecciones de la glándula mamaria bovina. Aunque se desconocen los factores de virulencia específicos involucrados en dicho proceso, se ha observado que las bacterias formadoras de biopelículas se asocian con infecciones persistentes. De igual forma se ha planteado que las bacterias móviles pueden ser más patógenas. El propósito del presente trabajo fue analizar la relación genética, capacidad de formación de biopelículas y movilidad de E. coli aislada de casos de mastitis bovina, así como la virulencia in vivo de aislados representativos. De acuerdo a los datos obtenidos en este trabajo el 67.7% de las bacterias pertenece al grupo filogenético A, el 17.6% al grupo B1 y el 14.7% al D. El estudio de la relación genética mediante (GTG)5 reveló que solo dos bacterias son idénticas genéticamente, el resto de las bacterias se organizaron en siete grupos distintos con 70% de similitud. Por otro lado, el 76.5% de las bacterias estudiadas fue capaz de formar biopelículas de forma fuerte, moderada o débil. Además, los genes csgA y fimA se detectaron en el 52.9% de las bacterias formadoras de biopelículas. Por su parte, el análisis de movilidad arrojó que el 70.6% fue móvil. Mientras que, el análisis de patogenicidad en larvas de Galleria mellonella reveló que aquellas bacterias formadoras de películas y móviles fueron capaces de matar un mayor número de larvas a las 24 h que las no formadoras. Los datos presentados en este trabajo indican que las E. coli causantes de mastitis bovina son muy diversas y pertenecen principalmente al grupo filogenético A y que las formadoras de biopelículas y móviles son más patógenas.

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“…It has been suggested that strains adapted to the mammary gland, called mammary pathogenic strains (MPEC), express virulence factors and more efficiently utilize nutrient sources that allow for improved survival within the mammary gland environment (Blum et al, 2015). However, a recent analysis (Leimbach et al, 2017) comparing the gene sequences of 8 mammary pathogenic strains and 6 fecal commensal strains did not reveal enrichment of virulence determinants or fitness factors in strains isolated from bovine mastitis cases versus commensal organisms. Instead, the authors suggest that each individual species contains unique species-specific traits that enhance fitness and are the result of competition for resources associated with commensalism rather than pathogenicity (Leimbach et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Variation in fibroblast expression of toll-like receptor 4 and lipopolysaccharide-induced cytokine production between animals predicts control of bacterial growth but not severity of Escherichia coli mastitis

Korkmaz

Elsasser

Kerr

2018

Journal of Dairy Science

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Mastitis caused by environmental pathogens such as Escherichia coli is highly problematic to the dairy industry because it incurs substantial cost and tends to be difficult to manage. An effective innate immune response by the host is key to controlling infection, but it should also limit collateral damage to the mammary gland. Between-animal differences in mastitis severity have been attributed to variability in the innate response. In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production. Animals ranked as high and low responders (HR and LR, respectively) were then infected with the P4 strain of E. coli to determine how difference in rank would affect response to mastitis. All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection. The HR animals were also significantly more capable of limiting bacterial growth. No differences in post-infection milk production or concentrations of milk BSA were measured. The current study indicates that HR animals have an early upregulation in their innate response that is beneficial for bacterial clearance; however, they are equally susceptible to tissue damage caused by an exuberant response to the infection. The dermal fibroblast may be used in conjunction with other cell types to determine how the innate response is regulated to mitigate unnecessary injury to the mammary gland while still effectively clearing the pathogen.

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No evidence for a bovine mastitisEscherichia colipathotype

Abstract: Background: Escherichia coli bovine mastitis is a disease of significant economic importance in

Cited by 13 publications

References 159 publications

Symposium review: Intramammary infections—Major pathogens and strain-associated complexity

Symposium review: Intramammary infections—Major pathogens and strain-associated complexity

Relación genética, formación de biopelículas, movilidad y virulencia de Escherichia coli aislada de mastitis bovina

Variation in fibroblast expression of toll-like receptor 4 and lipopolysaccharide-induced cytokine production between animals predicts control of bacterial growth but not severity of Escherichia coli mastitis

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