No evidence of Legionella infection in general practice patients presenting with acute respiratory infections in The Netherlands

Diederen, Bram M. W.; Jong, Caroline M. A. de; Aarts, Ingrid; Peeters, Marcel; Gageldonk-Lafeber, Arianne B van; Wilbrink, B.; Zee, Anneke van der

doi:10.1111/j.1469-0691.2005.01112.x

Cited by 3 publications

(4 citation statements)

References 16 publications

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“…In our experience, PCR as a diagnostic in patients with severe CAP is a sensitive and specific tool in those who produce sputum. The high specificity is exemplified by a recent investigation conducted by our laboratory (Diederen et al, 2005). In this case-control study, nose and throat samples from patients presenting with acute respiratory tract infections and matched controls were analysed for the presence of Legionella spp.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of real-time PCR for the early detection of Legionella pneumophila DNA in serum samples

Diederen¹,

Jong²,

Marmouk³

et al. 2007

Journal of Medical Microbiology

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Legionella pneumonia can be difficult to diagnose. Existing laboratory tests all have shortcomings, especially in the ability to diagnose Legionnaires' disease (LD) at an early stage of the disease in a specimen that is readily obtainable. The aim of this study was to assess the performance of PCR as a rapid diagnostic method and to compare the results of different PCR assays of serum samples from patients with LD. Samples included 151 serum samples from 68 patients with proven LD and 60 serum samples from 36 patients with respiratory tract infections other than Legionella. PCR assays were based on the 5S rRNA gene, 16S rRNA gene and the mip gene. The samples from patients with infections caused by pathogens other than Legionella all tested negative in PCR. Among the patients with proven LD 54.4 % (37/68) tested positive in 5S rRNA PCR, 52.9 % (36/68) in mip gene PCR and 30.9 % (21/68) in 16S rRNA PCR in the first available serum sample. The association between threshold cycle value in 5S PCR positive serum samples (n=49) and C-reactive protein value was determined, and showed a strong negative correlation (Pearson correlation coefficient r="0.63, P<0.0001). In addition to existing tests for the diagnosis of LD, detection of Legionella DNA in serum could be a useful tool for early diagnosis of LD caused by any Legionella species and serogroup, and has the potential to provide a diagnosis in a time frame that could affect initial infection management.

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Section: Resultsmentioning

confidence: 99%

Evaluation of real-time PCR for the early detection of Legionella pneumophila DNA in serum samples

Diederen¹,

Jong²,

Marmouk³

et al. 2007

Journal of Medical Microbiology

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“…Sensitivity controls consisted of tenfold dilutions of L. pneumophila DNA ranging from 1000 to 10 fg. As an internal control, Phocid herpesvirus 1 was added to the samples to monitor processing as well as PCR inhibition (Diederen et al, 2005a).…”

mentioning

confidence: 99%

Detection and quantification of Legionella pneumophila DNA in serum: case reports and review of the literature

Diederen¹,

Jong²,

Kluytmans

et al. 2006

Journal of Medical Microbiology

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Legionella pneumonia can be difficult to diagnose. Existing laboratory tests all have shortcomings, especially the ability to diagnose all Legionella spp. at an early stage. Detection of Legionella DNA in serum can be a valuable tool for the diagnosis of Legionnaires' disease (LD). This report describes two patients with LD diagnosed by PCR using serum samples. In addition, quantification of L. pneumophila DNA using real-time PCR during the course of illness was carried out. The results obtained mirrored both the clinical condition and C-reactive protein values during the course of the illness. Quantification of Legionella DNA in serum using real-time PCR could be a valuable tool to monitor the effects of antimicrobial therapy in patients with LD.

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“…LD at a similar incidence in nonhospitalized CAP patients gives a 3 to 5 times higher incidence than those based on hospitalized CAP cases, although most of the additional cases do not require admission (von Baum et al 2008). However, Legionella was not detected (by PCR) in samples collected by general practitioners from the nose and throat of 230 persons with acute respiratory illness and 200 controls in the Netherlands (Diederen et al 2005). Data about LD in nonhospitalized cases of CAP in other countries seem to be lacking.…”

Section: 1 True Incidence Of Ldmentioning

confidence: 97%

“…Still, the detection of mild cases of LD and/or cases caused by other serogroups and other species is complicated, also because of the limitations of the culture methods. A more complete and rapid diagnosis can be achieved by the inclusion of PCR-based methods (Diederen et al 2005;von Baum et al 2008;Mentasi et al 2012). Improvements in reporting and surveillance can contribute to a further increase of the notification rate as well.…”

Section: 1 True Incidence Of Ldmentioning

confidence: 98%

Microbial Growth in Drinking-Water Supplies: Problems, Causes, Control and Research Needs

Kooij¹,

Wielen²

2013

wio

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No evidence of Legionella infection in general practice patients presenting with acute respiratory infections in The Netherlands

Cited by 3 publications

References 16 publications

Evaluation of real-time PCR for the early detection of Legionella pneumophila DNA in serum samples

Evaluation of real-time PCR for the early detection of Legionella pneumophila DNA in serum samples

Detection and quantification of Legionella pneumophila DNA in serum: case reports and review of the literature

Microbial Growth in Drinking-Water Supplies: Problems, Causes, Control and Research Needs

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