No Major Impact of Two Homologous Proteins Ly6C1 and Ly6C2 on Immune Homeostasis

Abstract

Ly6C comprises two homologous components of Ly6C1 and Ly6C2, and the expression of either of the Ly6C molecules defines unique functional subsets of monocytes. Ly6C is also expressed by other immune cell types, including Aire-expressing medullary thymic epithelial cells. Because the role of Ly6C expression in determining the functional subsets remains unclear, we generated mice deficient for both Ly6C1 and Ly6C2 with CRISPR-Cas9-mediated deletion. Mice deficient for Ly6C1/Ly6C2 showed no major alterations in the subsets and function of monocyte and other immune cells, including the cells involved in the dextran sulfate sodium salt-induced colitis model. By generating the mice deficient for Ly6C1 alone, we have also investigated the expression pattern of Ly6C1 and Ly6C2 in immune cells. Except for medullary thymic epithelial cells and CD4 single-positive T cells, immune cells predominantly expressed Ly6C2. Thus, despite the importance as a marker with a unique differential expression pattern, the Ly6C molecules have no major impact on determining the functional subsets and maintaining immune homeostasis. ImmunoHorizons, 2022, 6: 202-210.

Lin−PU.1dimGATA‐1− defines haematopoietic stem cells with long‐term multilineage reconstitution activity

“…58 The expression of the monocyte-related genes Elane, Ctsg, Prtn3 and Ly6c2 and the neutrophil-related gene Mpo are raised in MPP4, indicating the myeloid differentiation trajectory of this cluster. [59][60][61] The expression levels of the lymphoid-related genes Dntt and Il18rap are increased in the LMPP cluster, which is consistent with the lymphoid-primed characteristics of these cells 62,63 (Figure 1C).…”

“…In the MPP3 cluster, there is strong expression of the gene Hmgb2 , which has been shown as a regulator for HSC regeneration, 57 and Mki67 , which encodes a marker of proliferation 58 . The expression of the monocyte‐related genes Elane , Ctsg , Prtn3 and Ly6c2 and the neutrophil‐related gene Mpo are raised in MPP4, indicating the myeloid differentiation trajectory of this cluster 59–61 . The expression levels of the lymphoid‐related genes Dntt and Il18rap are increased in the LMPP cluster, which is consistent with the lymphoid‐primed characteristics of these cells 62,63 (Figure 1C).…”

Single‐Cell RNA Sequencing and Spatial Transcriptomics Reveal Pathogenesis of Meningeal Lymphatic Dysfunction after Experimental Subarachnoid Hemorrhage

“…Clusters were annotated with well‐known markers. The cells were classified into 16 major cell types (Figure 1C–E) including B cells marked by Cd79A , Cd79B , and Ms4a1 , [ 13 ] NKs marked by Ncr1 , Klrb1c , and Nkg7 , [ 14 ] macrophages marked by C1qb , Cd68 , Csf1r , and Mrc1 , [ 15 ] neutrophils marked by S100α9 , IL1β , and Csf3r , [ 16 ] monocytes marked by Ccr2 and Ly6c2, [ 17 ] T cells marked by Cd3d , Cd3e , and Cd247 , [ 18 ] pDCs marked by Cd209a and Cd74 , [ 19 ] mast cells marked by Fcer1a , Kit , and Ms4a2 , [ 20 ] erythroblasts marked by Ttr , Enpp2 , and Ptgds , erythrocyte marked by Car2 , Hba‐a1 , and Hbb‐bs , [ 21 ] mesenchymal cells marked by Col1a1 , Col1a2 , Col3a1 , and DCN , [ 13 ] epithelial cells identified by the expression of Birc5 and Ube2c , [ 22 ] endothelial cell marked by Pecam1 , Cdh5 , and Kdr , [ 13 ] forebrain neuron marked by Gng13 and S100α5 , [ 23 ] glial cell marked by S100b and Cdh2 , [ 24 ] and neuralprecursor cells marked by Cdh2 , Pde6g , and Gnb3 . [ 24 ] Although all 16 major cell types were presented in mLVs both in sham and SAH groups (Figure 1C), the dynamic changes of each cell type were different.…”