No Mycobacterium paratuberculosis detected in intestinal tissue, including Peyer's patches and lymph follicles, of Crohn's disease

Chiba, Mitsuro; Fukushima, Tsuneo; Horie, Yasuo; Iizuka, Masahiro; Masamune, Osamu

doi:10.1007/s005350050119

Cited by 49 publications

(25 citation statements)

References 26 publications

(53 reference statements)

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“…One study reported that the IS900 sequence was detected in both CD patients and in healthy control subjects (41), whereas other studies were unable to detect IS900 in CD patients (42,43). Suenaga et al reported that the anti- …”

Section: Bacterial Agents In Crohn's Diseasementioning

confidence: 99%

The Role of Bacterial Infection in the Pathogenesis of Inflammatory Bowel Disease

2004

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Section: Bacterial Agents In Crohn's Diseasementioning

confidence: 99%

The Role of Bacterial Infection in the Pathogenesis of Inflammatory Bowel Disease

2004

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“…14,16 However, many of these studies are conflicting and utilize a variety of laboratory techniques without culture confirmation. [19][20][21] Similarly, empiric clinical trials with antimycobacterial therapy have not conclusively demonstrated evidence for MAP as a causal agent in this disease. 16,22 However, the first culture-confirmed case of disseminated MAP infection in a patient with human immunodeficiency virus (HIV) has recently been reported.…”

Section: Introductionmentioning

confidence: 99%

Growth, Congo Red Agar Colony Morphotypes and Antibiotic Susceptibility Testing of Mycobacterium avium subspecies paratuberculosis

Parrish¹,

Ko²,

Dick³

et al. 2004

Clinical Medicine & Research

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OBJECTIVEMycobacterium avium subspecies (subsp.) paratuberculosis (MAP) is the causative agent of Johne's disease in ruminants and has been associated with Crohn's disease in humans. We sought to test growth rates and susceptibilities of various strains of MAP in two available growth media. METHODSUsing the BACTEC macrobroth radiometric growth system and Congo Red-staining agar media, we determined inherent differences in growth characteristics of three bovine and two human strains of MAP and compared susceptibility results obtained in each growth system. RESULTSSignificant differences were observed in growth rate as well as mycobactin J dependence between strains and between a laboratory-adapted isolate of the same strain in the macrobroth system. Similarly, colonial morphology and Congo Red staining on agar media were observed. Two strains, one human and one bovine, demonstrated a 100% rough transparent colony with white coloration on Congo Red agar, while one bovine isolate exclusively grew as a smooth opaque colony with red coloration on Congo Red agar. The remaining strains exhibited mixtures of these two colonial morphotypes on agar media. Comparative susceptibility results between the BACTEC radiometric macrobroth method and the agar proportionality method showed good correlation for most antibiotics/inhibitors tested. However, erratic or poor growth in the macrobroth system prevented minimal inhibitory concentration determinations for two bovine strains by this method. CONCLUSIONThis study demonstrates the variability in the colonial morphology of MAP on Congo Red agar as well as the correlation of antibiotic susceptibility results between the BACTEC macro broth method and the agar proportionality method. This study also emphasizes the need for the development of improved, standardized culture and susceptibility test methods for MAP.

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“…Others did not find evidence for a relation between Crohn's disease and the presence of M. avium subsp. paratuberculosis (4,5). Unfortunately, M. avium subsp.…”

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confidence: 99%

Use of Multilocus Variable-Number Tandem-Repeat Analysis for TypingMycobacterium aviumsubsp.paratuberculosis

Overduin

Schouls²,

Roholl

et al. 2004

J Clin Microbiol

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The etiology of Crohn's disease in humans is largely unknown. Clinical signs of Crohn's disease partly resemble the clinical picture of Johne's disease in ruminants caused by Mycobacterium avium subsp. paratuberculosis. Because of the high prevalence of these bacteria in (products of) ruminants and their remarkable thermostability, concern has been raised about the possible role of these bacteria in the pathogenesis of Crohn's disease. In an attempt to develop a molecular typing method to facilitate meaningful comparative DNA fingerprinting of M. avium subsp. paratuberculosis isolates from the human and animal reservoirs, multilocus variable-number tandem-repeat analysis (MLVA) was explored and compared to IS900 restriction fragment length polymorphism (RFLP) typing. MLVA typing subdivided the most predominant RFLP type, R01, into six subtypes and thus provides a promising molecular subtyping approach to study the diversity of M. avium subsp. paratuberculosis

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No Mycobacterium paratuberculosis detected in intestinal tissue, including Peyer's patches and lymph follicles, of Crohn's disease

Cited by 49 publications

References 26 publications

The Role of Bacterial Infection in the Pathogenesis of Inflammatory Bowel Disease

The Role of Bacterial Infection in the Pathogenesis of Inflammatory Bowel Disease

Growth, Congo Red Agar Colony Morphotypes and Antibiotic Susceptibility Testing of Mycobacterium avium subspecies paratuberculosis

Use of Multilocus Variable-Number Tandem-Repeat Analysis for TypingMycobacterium aviumsubsp.paratuberculosis

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