<p><strong>Background and Objective: </strong>Personality and blood group type are both heritable factors. Personality is the compound mannerism or constitution that forms an individual’s peculiar state of mind. Intelligence quotient (IQ) is the quantification of one’s aptness to reason and solve problems. There is contemplation on the interconnection of the ABO blood group with personality traits and IQ. This study aimed to uncover the relationship between standard personality traits, IQ levels, and the ABO blood group.<br /><strong>Methods: </strong>This cross-sectional descriptive study comprised 200 healthy individuals (faculty and students of a teaching hospital). The blood typing of the participants was carried out by the standard tube method and the Big Five Inventory, a personality questionnaire containing 60 questions and the Naglieri nonverbal ability test, a mental capacity testing form, with 20 nonverbal questions were distributed, which were solved by all participants in a given time. After obtaining the results, scoring for personality traits and the levels of their IQ were determined. Data were analysed to find associations between personality traits, IQ level, and the ABO blood group.<br /><strong>Results: </strong>There exists an association between the ABO blood group and one of the personality traits, i.e., agreeableness (p = 0.001), while no significant relationship was found between the ABO blood group and the remaining four personality traits or the IQ level of the participants (p > 0.05).<br /><strong>Conclusion: </strong>ABO blood grouping may determine distinct personality-wise characteristics of an individual which may or may not be related to the IQ levels. </p>