NOAEL-dose of a neonicotinoid pesticide, clothianidin, acutely induce anxiety-related behavior with human-audible vocalizations in male mice in a novel environment

Hirano, Tetsushi; Yanai, Shogo; Takada, Toru; Yoneda, Naoto; Omotehara, Takuya; Kubota, Naoto; Minami, Kiichi; Yamamoto, Arata; Mantani, Youhei; Yokoyama, Toshifumi; Kitagawa, Hiroshi; Hoshi, Nobuhiko

doi:10.1016/j.toxlet.2017.10.010

Cited by 65 publications

(60 citation statements)

References 52 publications

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“…However, several studies have reported the disappearance and mass death of honeybees in Europe, as well as in America and other countries, occurring since the 1990s [18,21,65], which has raised concerns about the secondary effects of these agents on non-targeted insects. It has also recently been reported that NNs have adverse effects on vertebrates for example, on the reproductive organs in reptiles [9], birds [26,60] and mammals [67], and the emotional behavior in mice [23,24,59,68]. In order to assess the risk of NNs, therefore, there is an urgent need for definitive information about their effects on mammals.…”

Section: Introductionmentioning

confidence: 99%

Immunotoxicity evaluation by subchronic oral administration of clothianidin in Sprague-Dawley rats

Onaru

Ohno

Kubo

et al. 2020

The Journal of Veterinary Medical Science

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Neonicotinoid pesticides (NNs) act as agonists on nicotinic acetylcholine receptors (nAChRs) of insects, and there has been concern about the effects of NNs on the health of mammals. Since nAChRs are expressed in immune cells, it is possible that NNs disturb the immune system. However, few reports have examined the immunotoxicity of clothianidin (CLO), a widely-used NN. Here, we report the effects of CLO on immune organs and type IV allergic reactions in ear auricles. We orally administered CLO at 0, 30 and 300 mg/kg/day (CLO-0, 30 and 300) to Sprague-Dawley rats for 28 days. The effects were evaluated by organ and body weights, histopathology, and immunohistochemistry (TCRαβ, CD4, CD8, CD11b, CD68, CD103). In addition, some cecal contents were subjected to preliminary gut microbiota analysis, because microbiota contribute to host homeostasis, including the immunity. Our results showed loose stool, suppression of body weight gain, significant changes in organ weights (thymus: decreased; liver: increased) and changes of the gut microbiota in the CLO-300 group. There were no obvious histopathological changes in immune organs. Granulomas of the ear auricles were found in one rat of each of the CLO-30 and 300 groups, but CLO had no apparent effect on the thickness or immunohistochemistry in the ear auricles. We present new evidence that CLO affects the thymus and intestine, and might enhance the local inflammatory response. These findings should contribute to the appropriate evaluation of the safety of NNs in the future.

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Section: Introductionmentioning

confidence: 99%

Immunotoxicity evaluation by subchronic oral administration of clothianidin in Sprague-Dawley rats

Onaru

Ohno

Kubo

et al. 2020

The Journal of Veterinary Medical Science

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“…Particularly, murine models have been used in the characterization of various effects of pesticides under controlled conditions. These comprise the evaluation of tolerance thresholds by determining the lethal dose 50 (LD 50 ) -the dose that kills 50% of the test population (Dell'Omo et al 2003;Hirano et al 2017)and adverse effects by endocrine disruption (Bhaskar and Mohanty 2014;Ghodrat et al 2014), oxidative stress (Zhao et al 2016; Morales-Prieto and Abril 2017; Latchoumycandane and Mathur 2002), genotoxic effects (Peris-Sampedro et al 2016), or neurotoxic effects (Chen et al 2016;Dell'Omo and Shore 1996;Westlake et al 1983). All these studies have involved the application of various doses of one or more (mixtures) pesticides.…”

Section: Methodsmentioning

confidence: 99%

Presence of organochlorine pesticides and characterization of biomarkers in wild mice living in crop fields

Herrera

Escalona‐Segura

González-Jaurégui

et al. 2018

Therya

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The use of pesticides in crops bordering conservation areas poses risks for wildlife incidentally exposed; its effects in the Yucatan Peninsula, Mexico, are still unknown. Wild mice that inhabit farming land play a key ecological role, and can also be used as bioindicators of wildlife exposure to pollutants. The objectives of this work were to determine the presence of organochlorine pesticides (OC) in liver and evaluate the seasonal response of enzymatic biomarkers (BM) such as acetylcholinesterase (AChE), glutathione-S-transferase (GST), and catalase (CAT). Wild mice (Mus musculus) were captured between June 2015 and April 2016 in a watermelon crop of a rural community in Quintana Roo, Mexico. Individual mice were sacrificed in situ, followed by tissue dissection (liver, brain and skeletal muscle). Pesticides were determined by gas chromatography; BM activity was estimated by spectrophotometry. We captured 35 individuals, with a capture success of 2.33%. The prevailing OCs detected were drines in both climatic seasons. The rainy season influenced the activity of biomarkers to a greater extent, since AChE showed a lower activity (16 % and 40 % in brain and liver, respectively). GST was activated during the same season (77 % higher), while CAT did not show significant differences between seasons. There was no significant correlation between OC concentrations and biomarker activity, except for drines and AChE in the brain. OC concentrations recorded in the present work are below (2-and 20-fold lower) those reported in other works on rodents under controlled conditions. BM activity suggests that rainfall seem to exacerbate the effects of pesticides on mice; however, it seemingly does not pose a risk for their survival. The use of wild mice as bioindicators is a valuable and practical tool to detect disturbances derived from the use of pesticides in agricultural areas. Further research is recommended using a broader BM battery to identify those pollutants with the most severe effect on the physiology of wild animals incidentally exposed to pesticides. El uso de plaguicidas en cultivos aledaños a áreas de conservación, significa un riesgo para la fauna silvestre expuesta accidentalmente cuyos efectos son desconocidos, particularmente en la Península de Yucatán, México. Los ratones silvestres que habitan las regiones agrícolas, desempeñan un papel ecológico importante, además pueden servir como bioindicador de exposición a contaminantes. Los objetivos del presente trabajo fueron determinar la presencia de plaguicidas organoclorados (OC) en hígado y evaluar la exposición a partir de la respuesta temporal de los biomarcadores enzimáticos (BM), tales como acetilcolinesterasa (AChE), glutatión-S-transferasa (GST) y catalasa (CAT). Ratones silvestres (Mus musculus) fueron capturados entre junio de 2015 y abril de 2016 en un cultivo de sandía de una comunidad agrícola, en Quintana Roo, México. Los individuos fueron sacrificados in situ y se le diseccionaron los tejidos (hígado, cerebro y músculo esquelético). La presencia d...

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“…Male C57BL/6N mice exposed to a single dose of noobserved-adverse-effect level (NOAEL) of clothiadin showed elevated anxiety and increase in c-fos immunoreactivity in certain brain regions (123). Imidacloprid was also reported to stimulate high-fat-dietinduced adiposity and insulin resistance in mice (124).…”

Section: Neonicotinoid Effects On Vertebratesmentioning

confidence: 99%

Non-target toxicity of novel insecticides

Mužinić

Želježić

2018

Archives of Industrial Hygiene and Toxicology

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Humans have used insecticides since ancient times. The spectrum and potency of available insecticidal substances has greatly expanded since the industrial revolution, resulting in widespread use and unforeseen levels of synthetic chemicals in the environment. Concerns about the toxic effects of these new chemicals on non-target species became public soon after their appearance, which eventually led to the restrictions of use. At the same time, new, more environmentally-friendly insecticides have been developed, based on naturally occurring chemicals, such as pyrethroids (derivatives of pyrethrin), neonicotinoids (derivatives of nicotine), and insecticides based on the neem tree vegetable oil (Azadirachta indica), predominantly azadirachtin. Although these new substances are more selective toward pest insects, they can still target other organisms. Neonicotinoids, for example, have been implicated in the decline of the bee population worldwide. This review summarises recent literature published on non-target toxicity of neonicotinoids, pyrethroids, and neem-based insecticidal substances, with a special emphasis on neonicotinoid toxicity in honeybees. We also touch upon the effects of pesticide combinations and documented human exposure to these substances.

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NOAEL-dose of a neonicotinoid pesticide, clothianidin, acutely induce anxiety-related behavior with human-audible vocalizations in male mice in a novel environment

Cited by 65 publications

References 52 publications

Immunotoxicity evaluation by subchronic oral administration of clothianidin in Sprague-Dawley rats

Immunotoxicity evaluation by subchronic oral administration of clothianidin in Sprague-Dawley rats

Presence of organochlorine pesticides and characterization of biomarkers in wild mice living in crop fields

Non-target toxicity of novel insecticides

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