Nobiletin alleviates vascular alterations through modulation of Nrf-2/HO-1 and MMP pathways in<scp>l</scp>-NAME induced hypertensive rats

Potue, Prapassorn; Wunpathe, Chutamas; Maneesai, Putcharawipa; Kukongviriyapan, Upa; Prachaney, Parichat; Pakdeechote, Poungrat

doi:10.1039/c8fo02408a

Cited by 49 publications

(28 citation statements)

References 63 publications

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“…In L-NAMEtreated rats, the BW, as well as the weight of testes and epididymides, did not differ from those in normal rats, as reported in a previous study (Adedara et al, 2018). It is well accepted that chronic treatment with L-NAME produces systemic vasoconstriction and high blood pressure because of decreased NO bioavailability (Potue et al, 2019). Testicular dysfunction induced by hypertension is associated with structural alterations, and reduced serum testosterone levels and StAR protein expression.…”

Section: Discussionsupporting

confidence: 74%

“…The right testis was cut and homogenised with lysis buffer to extract proteins that were used to detect the protein expression of endothelial nitric oxide synthase (eNOS), Nrf2, HO-1 and StAR, as previously described (Chiangsaen et al, 2020;Potue et al, 2019). In each band, the protein intensities were observed and captured using an ImageQuant™400 imager (GE Healthcare).…”

Section: Western Blot Analysismentioning

confidence: 99%

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Cratoxylum formosum dyer extract alleviates testicular damage in hypertensive rats

et al. 2020

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Hypertension is a chronic disorder that contributes to cardiovascular disease (World Health, 2009). Currently, it is the major cause of morbidity and mortality in people worldwide (James et al., 2014). Hypertension associated with sexual dysfunction has been reported in male patients with hypertension (Fogari et al., 2001). Additionally, decreased sexual activity has been observed in male patients with essential hypertension, which has been associated with reduced testosterone levels (Fogari et al., 2002). A previous study has reported that serum total testosterone and epididymal sperm motility are significantly reduced in rats with nitric oxide (NO) depletion (Adedara et al., 2018). It is well known that testosterone is an essential hormone for the initiation and maintenance of spermatogenic processes (Chauhan et al., 2007), synthesised by testicular Leydig cells using cholesterol as its substrate (Li et al., 2011). The biosynthesis of testosterone requires steroidogenic acute regulatory protein (StAR), a mitochondrial cholesterol transporter, that transfers lipids from outside the mitochondrial membrane to the inside (Stocco, 2001). Reportedly,

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Section: Discussionsupporting

confidence: 74%

Section: Western Blot Analysismentioning

confidence: 99%

Cratoxylum formosum dyer extract alleviates testicular damage in hypertensive rats

et al. 2020

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“…Impairment of endothelium-dependent vasodilation in rats induced by L-NAME linked with reductions of eNOS protein expression and plasma nitrate/nitrite concentrations have been shown [ 5 ]. Moreover, aortic hypertrophy, including increases in vascular wall thickness, cross-sectional area, and wall-to-lumen ratio occurred after chronic L-NAME administration was shown [ 6 , 7 ]. Renin angiotensin system (RAS) activation characterized by increases in angiotensin converting enzyme activity and systemic angiotensin II (Ang II) concentration has also been observed in hypertensive rats with NO depletion [ 3 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Syzygium gratum Extract Alleviates Vascular Alterations in Hypertensive Rats

et al. 2020

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Background and Objectives: Syzygium gratum (SG) is a local vegetable and widely consumed in Thailand. Previously, a strong antioxidative effect of SG extract has been reported. The effects of SG extract on hypertension have remained unknown. The effect of SG aqueous extract on blood pressure and vascular changes were examined in L-NAME-induced hypertensive rats (LHR), and its potential active constituents were also explored. Materials and Methods: Male Sprague Dawley rats were allocated to control, L-NAME (40 mg/kg/day), L-NAME + SG (100, 300, 500 mg/kg/day), or captopril (5 mg/kg/day) groups. The components of SG extract were analyzed. Results: The analysis of aqueous SG extract was carried out using HPLC-Mass spectroscopy, and phenolic compounds could be identified as predominant components which might be responsible for its antihypertensive effects observed in the LHR model (p < 0.05). Additionally, SG extract also improved vascular responses to acetylcholine and decreased vascular remodeling in LHR (p < 0.05). Enhancements of eNOS expression and plasma nitric oxide metabolite levels, and attenuation of angiotensin converting enzyme (ACE) activity and plasma angiotensin II levels were observed in the LHR group treated with SG. Moreover, SG exhibited strong antioxidant activities by reducing vascular superoxide generation and systemic malondialdehyde in LHRs. Captopril suppressed high blood pressure and alleviated vascular changes and ACE activity in LHRs, similar to those of the SG extract (p < 0.05). Conclusion: Our results suggest that the SG extract exhibited antihypertensive effects, which is relevant to alleviation of vascular dysfunction and vascular remodeling of LHRs. These effects might be mediated by phenolic compounds to inhibit ACE activity and scavenge reactive oxygen species in LHR.

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“…In the cerebral ischemia model, nobiletin protected by upregulating Nrf‐2, HO‐1 expressions . Potue et al indicated that nobiletin activates the Nrf‐2/HO‐1 pathway in hypertensive rats. According to our study, we observed that systemic administration of nobiletin in group 4 significantly increased the expression of Nrf‐2 and HO‐1 in the liver and kidney compared to the only APAP administered group, suggesting that activation of the Nrf‐2/ARE pathway and HO‐1 upregulation could be a potential hepatoprotective and nephroprotective mechanism.…”

Section: Discussionmentioning

confidence: 99%

Nobiletin attenuates acetaminophen‐induced hepatorenal toxicity in rats

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et al. 2019

J Biochem & Molecular Tox

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The study aimed to examine the effects of nobiletin on the toxicity model induced with acetaminophen (APAP). For this purpose, 24 adult male rats were equally divided into four groups. The groups were the control group (group 1); dimethyl sulfoxide only, the APAP group (group 2) received a single dose of APAP 1000 mg/kg on the 10th day of experiment; the Nobiletin group (group 3), nobiletin (10 mg/kg) for 10 days; and the APAP + Nobiletin group (group 4), nobiletin (10 mg/kg) for 10 days with a single dose of APAP (1000 mg/kg) administered on the 10th day and the experiment ended after 48 hours. At the end of the study, a significant increase in malondialdehyde, interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) levels and a significant decrease in glutathione levels, glutathione peroxidase activities and nuclear factor erythroid-derived 2-like 2 (Nrf-2) and heme oxygenase-1 (HO-1) expressions were observed with APAP application in liver and kidney tissues. Serum aspartate transaminase (AST), alanine transaminase (ALT), urea, and creatinine levels were also significantly increased in the APAP group. However, nobiletin treatment in group 4 reversed oxidative stress and inflammatory and histopathological signs caused by APAP. It is concluded that nobiletin may be a beneficial substance that confers hepatorenal protection to APAP-induced toxicity via antioxidant and anti-inflammatory mechanisms. K E Y W O R D Sacetaminophen, nobiletin, Nrf-2/HO-1, oxidative stress

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Nobiletin alleviates vascular alterations through modulation of Nrf-2/HO-1 and MMP pathways inl-NAME induced hypertensive rats

Abstract: Nobiletin alleviates l-NAME-induced vascular dysfunction and remodeling and superoxide production in rats.

Cited by 49 publications

References 63 publications

Cratoxylum formosum dyer extract alleviates testicular damage in hypertensive rats

Cratoxylum formosum dyer extract alleviates testicular damage in hypertensive rats

Syzygium gratum Extract Alleviates Vascular Alterations in Hypertensive Rats

Nobiletin attenuates acetaminophen‐induced hepatorenal toxicity in rats

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