Nobiletin suppresses adipocyte differentiation of 3T3-L1 cells by an insulin and IBMX mixture induction

Kanda, Kota; Nishi, Kosuke; Kadota, Ayumu; Nishimoto, Sogo; Liu, Ming‐Cheh; Sugahara, Takuya

doi:10.1016/j.bbagen.2011.11.015

Cited by 70 publications

(48 citation statements)

References 30 publications

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“…However, nonstressed rats treated with nobiletin did not adopt in our present study, data concerning behavioral and biochemical changes in nonstressed rats would certainly provide some additional explanations on the antidepressant-like mechanism of nobiletin; so this is one of the limitations of the present study. Finally, since enhanced induction expression of BDNF in response to chronic antidepressant agent treatments could promote neuronal survival and protect neurons from the damaging effects of stress in rat brain [36], BDNF/TrkB-dependent neurogenesis involved in the antidepressant-like mechanism of nobiletin will be further investigated by using inhibitors/activators or gene block-out/silence technique.…”

Section: Discussionmentioning

confidence: 99%

Nobiletin Ameliorates the Deficits in Hippocampal BDNF, TrkB, and Synapsin I Induced by Chronic Unpredictable Mild Stress

Zhou

Liu

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Background. Our previous study has demonstrated that nobiletin could reverse the behavioral alterations in stressed mice. However, the relation of its antidepressant-like action with neurotrophic molecular expression remains unknown. This study aimed to explore the antidepressant-like mechanism of nobiletin related to the neurotrophic system in rats exposed to chronic unpredictable mild stress (CUMS). Methods. Depressive-like anhedonia (assessed by sucrose preference) and serum corticosterone secretion were evaluated in the CUMS, followed by brain-derived neurotrophic factor (BDNF), its tropomyosin-related kinase receptor B (TrkB), and the downstream target synapsin I expressions in the hippocampus. Results. Anhedonia, which occurred within week 2, was rapidly ameliorated by nobiletin. While fluoxetine needed additional 2 weeks to improve the anhedonia. In addition, nobiletin administration for 5 weeks significantly ameliorated CUMS-induced increase in serum corticosterone levels. Furthermore, we also found that CUMS-induced deficits of hippocampal BDNF, TrkB, and synapsin I were ameliorated by nobiletin. Conclusions. Taken together, these findings suggest that nobiletin produces rapidly acting antidepressant-like responses in the CUMS and imply that BDNF-TrkB pathway may play an important role in the antidepressant-like effect of nobiletin.

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Section: Discussionmentioning

confidence: 99%

Nobiletin Ameliorates the Deficits in Hippocampal BDNF, TrkB, and Synapsin I Induced by Chronic Unpredictable Mild Stress

Zhou

Liu

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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“…The key function of STAT5B is to mediate the effects of growth hormone [38, 39]. Modulation of STAT5 levels or transcriptional activity has already been described in cells treated with natural compounds such as nobiletin, a citrus flavonoid [40], thea flavins [41], and silibinin, a natural polyphenolic flavonoid which is a major bioactive component of silymarin isolated from Silybum marianum [42]. Furthermore, it has been reported that butein, the major biologically active polyphenolic component of the stems of Rhus verniciflua , downregulated the expression of STAT3-regulated gene products such as Bcl-xL, Bcl-2, cyclin D1, and Mcl-1 [43].…”

Section: Discussionmentioning

confidence: 99%

Coffee Polyphenols Change the Expression of STAT5B and ATF-2 Modifying Cyclin D1 Levels in Cancer Cells

Oleaga

Ciudad

Noé

et al. 2012

Oxidative Medicine and Cellular Longevity

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Background. Epidemiological studies suggest that coffee consumption reduces the risk of cancer, but the molecular mechanisms of its chemopreventive effects remain unknown. Objective. To identify differentially expressed genes upon incubation of HT29 colon cancer cells with instant caffeinated coffee (ICC) or caffeic acid (CA) using whole-genome microarrays. Results. ICC incubation of HT29 cells caused the overexpression of 57 genes and the underexpression of 161, while CA incubation induced the overexpression of 12 genes and the underexpression of 32. Using Venn-Diagrams, we built a list of five overexpressed genes and twelve underexpressed genes in common between the two experimental conditions. This list was used to generate a biological association network in which STAT5B and ATF-2 appeared as highly interconnected nodes. STAT5B overexpression was confirmed at the mRNA and protein levels. For ATF-2, the changes in mRNA levels were confirmed for both ICC and CA, whereas the decrease in protein levels was only observed in CA-treated cells. The levels of cyclin D1, a target gene for both STAT5B and ATF-2, were downregulated by CA in colon cancer cells and by ICC and CA in breast cancer cells. Conclusions. Coffee polyphenols are able to affect cyclin D1 expression in cancer cells through the modulation of STAT5B and ATF-2.

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“…Differentiation of 3T3-L1 preadipocytes into mature adipocytes was conducted as reported previously (Kanda et al, 2012). Briefly, 2 days after reaching confluence, 3T3-L1 preadipocytes grown in individual wells of collagen-coated 6-well cell culture plates (BD Falcon, Franklin Lakes, NJ) were subjected to induction by replacing the culture medium with the MDI induction media (DMEM medium containing 10% fetal bovine serum [FBS], 5 µg/ml of insulin [Sigma, St. Louis, MO], 0.5 mM IBMX [Sigma], and 10 µM DEX [Sigma]), and the induction proceeded for 2 days.…”

Section: Methodsmentioning

confidence: 99%

Visceral White Adipose Tissue is Susceptible to Alcohol-Induced Lipodystrophy in Rats: Role of Acetaldehyde

Zhang

Zhong

Sun

et al. 2015

Alcohol Clin Exp Res

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Background Chronic alcohol exposure causes lipid dyshomeostasis at the adipose-liver axis, reducing lipid storage in white fat and increasing lipid deposit in the liver. Previous studies have shown that visceral fat, rather than subcutaneous fat, is a risk factor for metabolic diseases. This study was conducted to determine whether chronic alcohol exposure differentially affects lipid metabolism in visceral (epididymal) and subcutaneous fat, and the mechanisms underlying the alcohol effects. Methods Male Wistar rats were pair-fed the Lieber-DeCarli control or alcohol liquid diet for 12 weeks to determine the effects of alcohol on the white fat. Tissue explants culture and 3T3-L1 culture were conducted to define the role of acetaldehyde in alcohol-induced adipose tissue dysfunction. Results Chronic alcohol feeding significantly reduced visceral fat mass and down-regulated peroxisome proliferator activator receptor-γ and CCAAT/enhancer binding protein-α, 2 important transcription factors in regulation of lipogenesis. The protein levels of lipogenic enzymes including phospho-ATP-citrate lyase, acetyl-CoA carboxylase, fatty acid synthase, lipin1, and diacylglycerol acyltransferase 2 in the visceral fat were reduced. In contrast, chronic alcohol exposure did not affect subcutaneous fat mass and had less effect on the protein levels of lipogenic enzymes and regulators. Accordingly, the visceral fat showed a lower protein level of aldehyde detoxification enzymes compared to the subcutaneous fat. Acetaldehyde treatment to either visceral fat explants or 3T3-L1 adipocytes produced similar effects on lipogenic enzymes and regulators as observed in animal model. Conclusions These results demonstrated that visceral fat is more susceptible to alcohol toxicity compared to subcutaneous fat, and disruption of adipose lipogenesis contributes to the pathogenesis of alcoholic lipodystrophy.

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Nobiletin suppresses adipocyte differentiation of 3T3-L1 cells by an insulin and IBMX mixture induction

Cited by 70 publications

References 30 publications

Nobiletin Ameliorates the Deficits in Hippocampal BDNF, TrkB, and Synapsin I Induced by Chronic Unpredictable Mild Stress

Nobiletin Ameliorates the Deficits in Hippocampal BDNF, TrkB, and Synapsin I Induced by Chronic Unpredictable Mild Stress

Coffee Polyphenols Change the Expression of STAT5B and ATF-2 Modifying Cyclin D1 Levels in Cancer Cells

Visceral White Adipose Tissue is Susceptible to Alcohol-Induced Lipodystrophy in Rats: Role of Acetaldehyde

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