Nocardia camponoti sp. nov., an actinomycete isolated from the head of an ant (Camponotus japonicas Mayr)

Liu, Chongxi; Guan, Xuejiao; Yao, Li; Li, Wenchao; Ye, Lan; Kong, Xiangxing; Song, Jia; Wang, Xiangjing; Xiang, Wensheng

doi:10.1099/ijsem.0.000963

Cited by 16 publications

(5 citation statements)

References 26 publications

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“…2). However, BLAST-calculated percent identities with previously described types of Nocardia strains, isolated from ants, Nocardia lasii 3C-HV12 T [Liu et al, 2016a] and Nocardia camponoti 1H-HV4 T [Liu et al, 2016b], were 99.46% and 97.54% respectively (Fig. 2).…”

Section: Resultsmentioning

confidence: 79%

“…First, this is corroborated by the stable isolation of mycelial prokaryotes from adult L. niger, regardless of the location of the nest. Second, the cultivated actinomycetes are localized in the heads of these insects, similarly to Lasius fuliginosus (Latreille, 1798) [Liu et al, 2016a;Ye et al, 2017] and Camponotus japonicus Mayr, 1866 [Li et al, 2016;Liu et al, 2016b;Cao et al, 2017;Jiang et al, 2018]. Third, the isolated streptomycetes have a broad-spectrum antibiotic activity and are able to inhibit the growth of widespread entomopathogenic microbes.…”

Section: Discussionmentioning

confidence: 99%

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Probable protective mission of actinomycetes associated with black garden ants, Lasius niger (Linnaeus, 1758) (Hymenoptera: Formicidae)

Zakalyukina,

Alferova,

Biryukov

2023

euroasentj

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A collection of mycelial bacteria of the phylum Actinomycetota was isolated from adult working individuals o f the ant Lasius niger (Linnaeus, 1758) selected on the territory of Moscow by plating on nutrient media. It is shown that cultured members of actinomycete communities are confi ned to the heads of insects, and belong to the genera Nocardia Trevisan, 1889 and Streptomyces Waksman and Henrici, 1943. Among the strains isolated from ants within the same family, producers of antibacterial compounds prevail over antifungal ones. Actinomycetes with antibacterial activity inhibit development of entomopathogenic bacteria Bacillus thuringiensis VKPM B-6650 and Paenibacillus alvei VKM B-502, as well as growth of the o pportunistic bacterium Staphylococcus aureus ATCC 25923, representing one of the most important human pathogens worldwide. At the same time, ability of individual strains to restrain the growth of entomopathogenic fungi Beauveria bassiana VKPM F-1357, Conidiobolus coronatus VKPM F-1359, as well as clinic ally signifi cant yeast Candida albicans ATCC 14053 was noted. Further studies are required to clarify the localization of possible symbionts and their ability to synthesize antimicrobial metabolites in vivo. РЕЗЮМЕ. Из взрослых рабочих особей муравья Lasius niger (Linnaeus, 1978), отобранных на территории г. Москвы, методом посева на питательные среды выделена коллекция мицелиальных представителей филума Actinomycetota. Показано, что культивируемые участники актиномицетных сообществ приурочены к головному отделу насекомых, а в таксономическом отношении представлены родами Nocardia Trevisan, 1889 и Streptomyces Waksman et Henrici, 1943. Среди штаммов, выделенных из муравьев внутри одной семьи, превалируют продуценты антибактериальных, а не антигрибных соединений. Актиномицеты с антибактериальной активностью подавляют развитие энтомопатогенных бактерий Bacillus thuringiensis VKPM B-6650 и Paenibacillus alvei VKM B-502, а также рост оппортунистической бактерии Staphylococcus aureus ATCC 25923, одного из наиболее важных патогенов человека по всему миру. В то же время отмечена способность отдельных штаммов к сдерживанию роста энтомопатогенных грибов Beauveria bassiana VKPM F-1357, Conidiobolus coronatus VKPM F-1359, а также клинически значимых дрожжей Candida albicans ATCC 14053. Требуются дальнейшие исследования для уточнения локализации возможных симбионтов и способности их синтезировать антимикробные метаболиты in vivo.

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Section: Resultsmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Probable protective mission of actinomycetes associated with black garden ants, Lasius niger (Linnaeus, 1758) (Hymenoptera: Formicidae)

Zakalyukina,

Alferova,

Biryukov

2023

euroasentj

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“…This high diversity of actinomycetes in C. lasiselene is consistent with previous reports. In the past decade, several new actinomycete species have been isolated from Camponotus ants, including Amycolatopsis camponoti from Camponotus vagus [50] , Streptomyces capitiformicae , Streptomyces camponoticapitis , Streptomyces formicae and Nocardia camponoti from the heads of Camponotus japonicus [51] – [54] , as well as Promicromonospora alba isolated from the cuticle of C. japonicus [55] . Furthermore, some bioactive compounds were also isolated from the bacteria isolated from Camponotus ant such as nybomycin from Streptomyces isolated from C. vagus [56] , deinococcucins A-D, aminoglycolipids, from Deicococcus sp.…”

Section: Discussionmentioning

confidence: 99%

Diversity and antimicrobial activity of the tropical ant-derived actinomycetes isolated from Thailand

Tunvongvinis,

Jaitrong,

Samung

et al. 2024

AIMSMICRO

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<abstract> Antibiotic resistance is one of the most important global healthcare challenges and is responsible for the mortality of millions of people worldwide every year. It is a crisis attributed to misuse of antibiotics and a lack of new drug development. Actinomycetes constitute a group of Gram-positive bacteria known for their distinctive high guanine-cytosine (G+C) content in their genomic DNA. These microorganisms are widely recognized for their capability to generate a wide range of secondary metabolites with diverse biological activities. These versatile microorganisms are ubiquitous in diverse ecosystems, including soil, freshwater, marine sediments, and within the bodies of insects. A recent study has demonstrated that social insects, such as ants, host a diverse array of these bacteria. In this study, we involved the isolation and characterization of a total of 72 actinomycete strains obtained from 18 distinct ant species collected from various regions across Thailand. Utilizing 16S rRNA gene analysis, these isolated actinomycetes were classified into four distinct genera: <italic>Amycolatopsis</italic> (2 isolates), <italic>Micromonospora</italic> (1 isolate), <italic>Nocardia</italic> (8 isolates), and <italic>Streptomyces</italic> (61 isolates). Among the <italic>Streptomyces</italic> strains, 23 isolates exhibited antimicrobial activity against a panel of Gram-positive bacteria, including <italic>Bacillus subtilis</italic> ATCC 6633, <italic>Staphylococcus epidermidis</italic> ATCC 12228, <italic>Staphylococcus aureus</italic> ATCC 25923, <italic>Kocuria rhizophila</italic> ATCC 9341, and Methicillin-resistant <italic>Staphylococcus aureus</italic> (MRSA) DMST 20646. Additionally, two isolates displayed antifungal activity against <italic>Candida albicans</italic> TISTR 5554. Based on 16S rRNA gene sequence similarity studies, these two isolates, ODS25 and ODS28, were demonstrated to be closely related to <italic>Streptomyces lusitanus</italic> NBRC 13464T (98.07%) and <italic>Streptomyces haliclonae</italic> DSM 41970T (97.28%), respectively. The level of 16S rRNA gene sequence similarity below 98.65% cutoff indicates its potential as a novel actinomycete species. These findings underscore the potential of actinomycetes sourced from ants as a valuable reservoir of novel antimicrobials. </abstract>

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“…and Promicromonospora alba sp. were recently isolated from the heads of these ants, and their genomes were sequenced (Guo et al, 2016; Liu et al, 2016). While the NP repertoire of these strains has not yet been explored, further study of these newly reported interactions might lead to the discovery of novel bioactive compounds that play a role in shaping the Japanese carpenter ant’s ecosystem.…”

Section: Use Of Actinomycete Natural Products By Fungus-farming Antsmentioning

confidence: 99%

Molecules to Ecosystems: Actinomycete Natural Products In situ

et al. 2017

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Actinomycetes, filamentous actinobacteria found in numerous ecosystems around the globe, produce a wide range of clinically useful natural products (NP). In natural environments, actinomycetes live in dynamic communities where environmental cues and ecological interactions likely influence NP biosynthesis. Our current understating of these cues, and the ecological roles of NP, is in its infancy. We postulate that understanding the ecological context in which actinomycete metabolites are made is fundamental to advancing the discovery of novel NP. In this review we explore the ecological relevance of actinomycetes and their secondary metabolites from varying ecosystems, and suggest that investigating the ecology of actinomycete interactions warrants particular attention with respect to metabolite discovery. Furthermore, we focus on the chemical ecology and in situ analysis of actinomycete NP and consider the implications for NP biosynthesis at ecosystem scales.

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Nocardia camponoti sp. nov., an actinomycete isolated from the head of an ant (Camponotus japonicas Mayr)

Abstract: Nocardia camponoti sp. nov., an actinomycete isolated from the head of an ant (Camponotus japonicas Mayr)

Cited by 16 publications

References 26 publications

Probable protective mission of actinomycetes associated with black garden ants, Lasius niger (Linnaeus, 1758) (Hymenoptera: Formicidae)

Probable protective mission of actinomycetes associated with black garden ants, Lasius niger (Linnaeus, 1758) (Hymenoptera: Formicidae)

Diversity and antimicrobial activity of the tropical ant-derived actinomycetes isolated from Thailand

Molecules to Ecosystems: Actinomycete Natural Products In situ

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