Nocardia jiangxiensis sp. nov. and Nocardia miyunensis sp. nov., isolated from acidic soils

Abstract

The taxonomic positions of two soil actinomycetes, strains 117T and 43401T, provisionally assigned to the genus Nocardia were determined in a polyphasic study. The organisms were found to have phenotypic properties typical of members of the genus Nocardia, and formed a distinct branch in the Nocardia 16S rRNA gene tree. It was evident from the phylogenetic data that the isolates were most closely, albeit loosely, associated with the type strains of Nocardia nova and Nocardia pseudobrasiliensis. However, all four of these organisms were readily distinguishable from one another using DNA–DNA relatedness and phenotypic data. It is evident from the genotypic and phenotypic data that the two isolates should be recognized as novel species of the genus Nocardia. It is proposed, therefore, that strains 117T (=CGMCC 4.1904T=JCM 12860T) and 43401T (=CGMCC 4.1905T=JCM 12861T) be classified in the genus Nocardia as the type strains of Nocardia miyunensis sp. nov. and Nocardia jiangxiensis sp. nov., respectively.

Cutaneous Pyogranulomas Associated with Nocardia jiangxiensis in a Cat from the Eastern Caribbean

“…To our knowledge, this is the first report associating N. jiangxiensis with clinical disease in an animal, adding this species of Nocardia to the list of nocardial pathogens. N. jiangxiensis has been a documented species since 2005, when it was first discovered and isolated from rhizosphere soil of goose grass next to a copper mine in Wushan, Jiangxi Province, in southern China [9]. Upon its discovery, N. jiangxiensis was found to have to 98.7% and 97.9% 16S rRNA gene sequence similarity with N. nova JCM 6044 T and Nocardia pseudobrasiliensis ATCC 51512 T , respectively.…”

“…Upon its discovery, N. jiangxiensis was found to have to 98.7% and 97.9% 16S rRNA gene sequence similarity with N. nova JCM 6044 T and Nocardia pseudobrasiliensis ATCC 51512 T , respectively. This isolate had DNA-DNA relatedness values and corresponding relatedness values of 46% with N. nova JCM 6044 T and of 39% with N. pseudobrasiliensis ATCC 51512 T , values that are well below the recommended threshold of 70% for genomic species differentiation, and was thus deemed a novel species after full analysis (GenBank Accession number AY639902) [9]. The cat in this case had no travel history outside of the West Indies island chain in the Caribbean, so it is hypothesized that this species exists well outside southern China, and its global reach is yet to be determined.…”

Nocardia aciditolerans sp. nov., isolated from a spruce forest soil

“…Their phylogenetic relatives include their closest phylogenetic neighbour, the type strain of N. kruczakiae , a respiratory pathogen originally isolated from an immunocompromised patient (Conville et al 2004). The isolates shared even lower 16S rRNA gene similarities with N. jiangxiensis 43401 T (CGMCC 4.1905 T ) and N. miyunensis 117 T (CGMCC 4.1904 T ) , aciditolerant strains isolated from acidic soils on an acidified selective isolation medium (Cui et al 2005). It seems likely from these results that aciditolerant nocardiae may form an integral part of actinobacterial communities in acidic soils.…”

“…The tree was rooted using the rRNA gene sequence of Nocardia acidivorans GW4-1778 T (accession number AM40 2972). Similarly, an expanded neighbour-joining tree was generated based on almost complete 16S rRNA gene sequences of the isolates and corresponding sequences of the type strains of Nocardia species, including those of the two acidotolerant taxa, Nocardia jiangxiensis and Nocardia miyunensis (Cui et al 2005). …”

Nocardia acidivorans sp. nov., isolated from soil of the island of Stromboli

“…The genus Nocardia encompasses more than 40 established species of mycolic acid-containing actinomycetes, including recently described species (Albuquerque de Barros et al, 2003;Cui et al, 2005;Iida et al, 2006;Kageyama et al, 2004aKageyama et al, , b, c, 2005Kämpfer et al, 2004;Lee, 2006;Wang et al, 2004;Xu et al, 2005;Yamamura et al, 2005;Yassin & Brenner, 2005;Yassin et al, 2003;Zhang et al, 2003Zhang et al, , 2004. A comprehensive summary of the taxonomy of the genus has been given by Goodfellow et al (1999).…”