Nocardia noduli sp. nov., a novel actinobacterium with biotechnological potential

Nouioui, Imen; Pötter, Gabriele; Jando, Marlen; Goodfellow, Michael

doi:10.1007/s00203-022-02878-x

Cited by 4 publications

(3 citation statements)

References 52 publications

(57 reference statements)

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“…Plants and fungi produce terpene as part of their defense mechanism [ 44 ]. Currently, terpene synthase genes have been found to be distributed in bacteria such as Nonomuraea terrae [ 45 ], Caenimonas aquaedulcis [ 46 ], Panacibacter microcysteis [ 47 ], and Nocardia noduli [ 48 ], which may be useful for discovering new natural products [ 49 ]. The presence of NRPS in the genome of strain JC4 T suggested that this strain can produce antimicrobial, antiviral, anti-cancer, and anti-inflammatory compounds [ 50 ].…”

Section: Resultsmentioning

confidence: 99%

Mucilaginibacter aquariorum sp. nov., Isolated from Fresh Water

Kang

et al. 2022

J. Microbiol. Biotechnol.

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Phylogenetic AnalysisGenomic DNA was extracted from three-day-old cultures according to Ko et al. [16]. Amplification and sequencing of the 16S rRNA gene were performed using universal primer set 27F (5'-AGAGTTTGATCATGGCTCAG-3') and 1492R (5'-TACGGYTACCTTGTTACGACTT-3') [17]. The nearly full-length 16S rRNA gene sequence of strain JC4 T (1,402 bp) was compared with those of valid species in the EzTaxon server. Phylogenetic trees were A Gram-stain-negative, rod-shaped bacterial strain, JC4 T , was isolated from a freshwater sample and determined the taxonomic position. Initial identification based on 16S rRNA gene sequences revealed that strain JC4 T is affiliated to the genus Mucilaginibacter with a sequence similarity of 97.97% to Mucilaginibacter rigui WPCB133 T . The average nucleotide identity and digital DNA-DNA hybridization values between strain JC4 T and Mucilaginibacter species were estimated below 80.92% and 23.9%, respectively. Strain JC4 T contained summed feature 3 (C 16:1 ω7c and/or C 16:1 ω6c) and iso-C 15:0 as predominant cellular fatty acids. The dominant polar lipids were identified as phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, and two unidentified lipids. The respiratory quinone was MK-7. The genomic DNA G+C content of strain JC4 T was determined to be 42.44%. The above polyphasic evidences support that strain JC4 T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter aquariorum sp. nov. is proposed. The type strain is JC4 T (= KCTC 92230 T = LMG 32715 T ).

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Section: Resultsmentioning

confidence: 99%

Mucilaginibacter aquariorum sp. nov., Isolated from Fresh Water

Kang

et al. 2022

J. Microbiol. Biotechnol.

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“…Nocardia species often invade the human body from respiratory tract, oral mucosa lesions and skin lesions, and spread to lung, brain and other organs through respiratory tract and blood, which is easy to cause infection ( 11 ). Nocardial is aerobic, and the colonies were smooth and moist when cultured at 37° with general medium, grows into macroscopic colonies within 2–6 days, obtain satisfactory culture results requiring 4–6 weeks ( 12 , 13 ). The colonies varied in color, including cheese, yellow, pink, coral red, and orange red ( 14 , 15 ).…”

Section: Discussionmentioning

confidence: 99%

Case report: From oral infection to life-threatening pneumonia: clinical considerations in Nocardia infection from a case

Chen,

Wang,

Dong

et al. 2024

Front. Med.

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Nocardia is an anthropozoonotic bacteria that occurs widely in the natural environment. However, because it is a gram-positive aerobic opportunistic pathogen, it rarely occurs in patients with no prior history of immune function disease. Since the symptoms are nonspecific the diagnosis of Nocardia pneumonia is challenging. Previous studies have not reported that this anthropozoonotic bacteria colonizing the human body could cause severe pneumonia by gingival pain and pharyngeal discomfort. This case report describes a previously healthy 60-year-old female farmer who presented to the doctor with gingival pain and pharyngeal discomfort. She was treated with a dental cleaning and oral metronidazole. The patient rapidly progressed to breathing difficulties. Lung shadow was found by computerized tomography examination. The radiologist diagnosed pulmonary tuberculosis as image-based. Through laboratory examination and culture of pathogenic microorganisms in the sputum and blood of the patient, no obvious positive findings were found. The disease progressed rapidly to tracheal intubation ventilator assisted breathing. Subsequently, the patient underwent alveolar lavatory examination under endotracheal intubation fiberbronchoscopy, and the culture of alveolar lavage fluid indicated Nocardia. According to this result, the patient’s disease was quickly controlled after selecting the targeted drug compound sulfamethoxazole and intravenous meropenem for treatment. In view of the reason for the high misdiagnosis rate due to the low positive rate of Nocardia culture in most cases, the clinical thinking of diagnosis and treatment from oral infection symptoms to fatal pneumonia reported in this case has certain clinical popularization and enlighten significance, not only improved the diagnosis and treatment of rare diseases, but also be reduced medical disputes.

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“…Both have been shown to have biosynthetic potential for novel bioactive compounds 37 - 39 . Nocardia are known as pathogens of animals and humans that cause infections known as actinomycetoma and nocardiosis, and they are also saprophytes degrading organic matter 38 , 39 . Strains isolated from clinical cases are frequently investigated for their pathogenicity mechanism and less frequently to study their biosynthetic potential, as an important source of new drugs.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity of Nocardia spp. and Streptomyces sp. on multidrug-resistant pathogens causing neonatal sepsis

González-Nava,

Manzanares-Leal,

Zapi-Colín

et al. 2024

Rev. Inst. Med. trop. S. Paulo

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Neonatal sepsis leads to severe morbidity and occasionally death among neonates within the first week following birth, particularly in low- and middle-income countries. Empirical therapy includes antibiotics recommended by WHO. However, these have been ineffective against antimicrobial multidrug-resistant bacterial strains such as Klebsiella spp, Escherichia coli , and Staphylococcus aureus species. To counter this problem, new molecules and alternative sources of compounds with antibacterial activity are sought as options. Actinobacteria, particularly pathogenic strains, have revealed a biotechnological potential still underexplored. This study aimed to determine the presence of biosynthetic gene clusters and the antimicrobial activity of actinobacterial strains isolated from clinical cases against multidrug-resistant bacteria implicated in neonatal sepsis. In total, 15 strains isolated from clinical cases of actinomycetoma were used. PCR screening for the PKS-I, PKS-II, NRPS-I, and NRPS-II biosynthetic systems determined their secondary metabolite-producing potential. The strains were subsequently assayed for antimicrobial activity by the perpendicular cross streak method against Escherichia fergusonii Sec 23, Klebsiella pneumoniae subsp. pneumoniae H1064, Klebsiella variicola H776, Klebsiella oxytoca H793, and Klebsiella pneumoniae subsp. ozaenae H7595, previously classified as multidrug-resistant. Finally, the strains were identified by 16S rRNA gene sequence analysis. It was found that 100% of the actinobacteria had biosynthetic systems. The most frequent biosynthetic system was NRPS-I (100%), and the most frequent combination was NRPS-I and PKS-II (27%). All 15 strains showed antimicrobial activity. The strain with the highest antimicrobial activity was Streptomyces albus 94.1572, as it inhibited the growth of the five multidrug-resistant bacteria evaluated.

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Nocardia noduli sp. nov., a novel actinobacterium with biotechnological potential

Cited by 4 publications

References 52 publications

Mucilaginibacter aquariorum sp. nov., Isolated from Fresh Water

Mucilaginibacter aquariorum sp. nov., Isolated from Fresh Water

Case report: From oral infection to life-threatening pneumonia: clinical considerations in Nocardia infection from a case

Antibacterial activity of Nocardia spp. and Streptomyces sp. on multidrug-resistant pathogens causing neonatal sepsis

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