Nocardia salmonicida nom. rev., a fish pathogen

Işık, Kamil; Chun, Jongsik; Hah, Yung Chil; Goodfellow, Michael

doi:10.1099/00207713-49-2-833

Cited by 97 publications

(61 citation statements)

References 23 publications

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“…Acid-fastness tests were after a modification of the Ziehl-Neelsen method (Isik et al, 1999). The staining properties were observed microscopically (Axio Scope.A1 Vario; Zeiss).…”

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confidence: 99%

“…7H 2 O 0.05 g and FeSO 4 . 7H 2 O 0.05 g after 3 weeks of incubation at 28 u C. The purified cultures were maintained on tryptic soy agar (TSA) (Difco) at 4 u C and as glycerol suspensions (20 %, v/v) at 280 u C.The colonial properties of the test strains were determined on TSA and ISP medium 2 (Shirling & Gottlieb, 1966) (Difco) plates incubated at 28 u C. Smears from these plates were prepared, Gram-stained after Hucker's modification, as described by Isik et al (1999). Acid-fastness tests were after a modification of the Ziehl-Neelsen method (Isik et al, 1999).…”

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Williamsia sterculiae sp. nov., isolated from a Chinese medicinal plant

Fang

Wang

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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Two actinobacterial strains, CPCC 203464 T and CPCC 203448, isolated from surface-sterilized stems of medicinal plants were subjected to a polyphasic taxonomic study. These two aerobic organisms formed pale yellow colonies on tryptic soy agar (TSA). Cells were Gram-stain-positive, non-acid-fast, non-motile, rod-or coccoid-like elements. Comparative 16S rRNA gene sequence analysis indicated that strains CPCC 203464 T and CPCC 203448 were most closely related to the type strains of the species of the genus Williamsia. Chemotaxonomic properties such as containing meso-diaminopimelic acid in the cell wall, arabinose, galactose and ribose being the whole-cell hydrolysate sugars, phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylinositol (PI) as the phospholipids, and C 16 : 0 , 10-methyl C 18 : 0 , C 18 : 1 v9c, C 16 : 1 v7c and/or iso-C 15 : 0 2-OH as major fatty acids supported the affiliation of strains CPCC 203464 T and CPCC 203448 to the genus Williamsia. The DNA-DNA hybridization values in combination with differentiating chemotaxonomic and physiological characteristics strongly suggested that these two isolates should be classified as representatives of a novel species of the genus Williamsia. The name Williamsia sterculiae sp. nov. is proposed, with strain CPCC 203464 T (5DSM 45741 T 5KCTC 29118 T ) as the type strain.The genus Williamsia was classified as a group of mycolicacid-containing actinomycetes, with Gram-positive cells that were non-spore-forming short rods (Kämpfer et al., 1999). At the time of writing, the genus encompasses eight species with validly published names including Williamsia muralis (Kämpfer et al., 1999) (Sazak & Sahin, 2012). Strains of these species were isolated from diverse environments, such as human blood (Yassin & Hupfer, 2006), soil (Yassin et al., 2007, meadow hay (Jones et al., 2010), deep-sea sediment (Stach et al., 2004;Pathom-aree et al., 2006), indoor building materials (Kämpfer et al., 1999), leaf surface (Kämpfer et al., 2011) and lake sediment (Sazak & Sahin, 2012). Here, we report the results of polyphasic taxonomic study of two strains, designated CPCC 203464 T and CPCC 203448, isolated from surfacesterilized medicinal plants.Strain CPCC 203464 T was isolated from a surface-sterilized stem of Sterculia lychnophora Hance, the seeds of which are used in traditional Chinese medicine. While strain CPCC 203448 was isolated from a stem of Hibiscus sabdariffa Linn, the flowers and the seeds of which are also used in traditional Chinese medicine. These two medicinal plants were collected from Yunnan medicinal plant plantation, China. CPCC 203464 T was obtained on a tap water agar plate (agar 1.5 %; pH 7.2) and CPCC 203448 was isolated on another isolation medium plate containing (l 21 , pH 7.2) sodium propionate 2 g, NH 4 NO 3 0.1 g, KCl 0.1 g, MgSO 4 . 7H 2 O 0.05 g and FeSO 4 . 7H 2 O 0.05 g after 3 weeks of incubation at 28 u C. The purified cultures were maintained on tryptic soy agar (TSA) (Difco) at 4 u C and as...

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“…Acid-fastness tests were after a modification of the Ziehl-Neelsen method (Isik et al, 1999). The staining properties were observed microscopically (Axio Scope.A1 Vario; Zeiss).…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Williamsia sterculiae sp. nov., isolated from a Chinese medicinal plant

Fang

Wang

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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“…Gram staining was examined by Hucker's method (Gerhardt, 1981). Carbon source utilization was examined using well-established procedures (Isik et al, 1999) with the addition of 3 % NaCl. Nitrate reduction was determined after 7 and 14 days of growth by the addition of 0.2 ml each of Griess-Ilosvay reagents I and II to stab cultures in sloppy nitrate medium supplemented with 0.2 % (w/v) KNO 3 and 0.6 % Bacto agar (Difco) (Gordon & Mihm, 1962).…”

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confidence: 99%

Nocardiopsis nikkonensis sp. nov., isolated from a compost sample

Yamamura

Ohkubo

Ishida

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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An actinomycete strain, designated YU1183-22 T , was isolated from a compost sample collected in Nikko, Japan. The isolate formed white aerial mycelium with relatively long aerial hyphae showing chains of arthrospores. Strain YU1183-22 T grew with 0-10 % (w/v) NaCl, at pH 6-11 and at 10-37 6C (optimum 30 6C). Strain YU1183-22 T contained meso-diaminopimelic acid and no diagnostic sugars. The predominant menaquinones were MK-10(H 10 ) and ). The polar lipids were phosphatidylcholine and phosphatidylglycerol. The major cellular fatty acids were iso-C 16 : 0 , anteiso-C 17 : 0 and tuberculostearic acid. The G+C content of the DNA was 72.3 mol%. Chemotaxonomic and morphological characterization clearly demonstrated that strain YU1183-22 T belonged to the genus Nocardiopsis. Phylogenetic analysis using 16S rRNA gene sequences showed that the isolate was closely related to Nocardiopsis salina YIM 90010 T (98.0 % 16S rRNA gene sequence similarity), Nocardiopsis xinjiangensis YIM 90004 T (97.9 %) and Nocardiopsis kunsanensis HA-9 T (97.3 %). However, DNA-DNA relatedness as well as physiological and biochemical analyses showed that strain YU1183-22 T could be differentiated from its closest phylogenetic relatives. It is proposed that this strain be classified as a representative of a novel species of the genus Nocardiopsis, with the name Nocardiopsis nikkonensis sp. nov. The type strain is YU1183-22 T (5NBRC 102170 T 5KCTC 19666 T ).The genus Nocardiopsis is a member of the family Nocardiopsaceae (Meyer, 1976) and, at the time of writing, about 30 species have been described, including Nocardiopsis arabia, N. valliformis, N. quinghaiensis and N. ganjiahuensis (Hozzein & Goodfellow, 2008;Yang et al., 2008;Chen et al., 2008;Zhang et al., 2008). Many members of the genus Nocardiopsis have been isolated from hypersaline or alkaline environments. However, others have been isolated from non-hypersaline environments, including the sputum of a kidney transplant patient, the rhizosphere of Casuarina sp. and the atmosphere of a composting facility (Yassin et al., 1997; Evtushenko et al., 2000; Kämpfer et al., 2002). Nocardiopsis species are of value to industry as they are known to produce bioactive agents such as griseusin D, apoptolidin and methylpendolmycin (Li et al., 2007;Kim et al., 1997;Sun et al., 1991). Hence, the discovery of additional species of this genus will contribute both to an understanding of their ecological roles and to the provision of bioresources for industrial applications.Strain YU1183-22 T was isolated from a matured compost sample collected in Nikko, Japan, by growth on HV agar (Hayakawa & Nonomura, 1987) containing (l 21 ) 20 mg nalidixic acid, 50 mg cycloheximide and 100 g NaCl. After incubation at 28 u C for 14 days, colonies of strain YU1183-22 T showed a thin, flat morphology with sparse, white, aerial hyphae.Morphological features of cells of strain YU1183-22 T grown on HV agar and oatmeal-YGG agar (Hayakawa et al., 1982) at 28 u C for 14 days were analysed by light and scanning electron microsc...

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“…Acid-alcoholfastness was examined by using a modified version of the Ziehl-Neelsen method (Gordon, 1967) in which 0.5 % (v/v) sulfuric acid was used for decolorization. The hydrolysis of complex substrates and the utilization of carbon sources were examined by using well-established procedures (Gordon et al, 1974;Isik et al, 1999). The tests for aesculin and arbutin hydrolysis (Williams et al, 1983), nitrate reduction (Gordon & Mihm, 1962) and urea hydrolysis (Gordon et al, 1974) were performed using established procedures.…”

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confidence: 99%

Nocardia amamiensis sp. nov., isolated from a sugar-cane field in Japan

Yamamura

Tamura

Sakiyama

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

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An actinomycete, strain TT 00-78 T , was isolated from soil from a sugar-cane field on Amami Island in Japan, using an SDS/yeast extract pre-treatment method, and the taxonomy was studied using a polyphasic approach. The chemotaxonomic and morphological characterizations clearly demonstrated that the strain belongs to the genus Nocardia. 16S rRNA gene sequencing studies showed that the strain was closely related to the type strains of Nocardia pneumoniae (98.6 %), Nocardia araoensis (98.1 %), Nocardia arthritidis (97.9 %) and Nocardia beijingensis (97.7 %). However, the results of DNA-DNA hybridization and physiological and biochemical tests showed that strain TT 00-78 T could be differentiated from its closest phylogenetic relatives both genotypically and phenotypically. Therefore this strain represents a novel species of the genus Nocardia, for which the name Nocardia amamiensis sp. nov. is proposed. The type strain is TT 00-78 T (=NBRC 102102The genus Nocardia is a member of the family Nocardiaceae (Stackebrandt et al., 1997) and currently contains 63 species with validly published names, including the recently described species Nocardia jiangxiensis, Nocardia jejuensis and Nocardia harenae (Cui et al., 2005;Lee, 2006;Seo & Lee, 2006). In recent years, many species have been isolated from clinical specimens and are considered to be opportunistic pathogens in animal infections (Watanabe et al., 2006;Brown-Elliott et al., 2006). In contrast, the natural habitats of nocardiae are widely distributed throughout terrestrial and aquatic ecosystems, including, for instance, scumming activated sludge and sediments from moats, lakes and marine environments (Attwell & Colwell, 1981;Yamamura et al., 2005). Additionally, they are considered to play a significant role as saprophytic organisms in the turnover of naturally occurring organic substances . Nocardia species have industrial uses as well, as they are known to produce bioactive agents such as nocardicin, tubelactomicin A and brasilicardin A (Aoki et al., 1976;Komaki et al., 1999;Igarashi et al., 2000).Strain TT 00-78 T was isolated from soil in a sugar-cane field on Amami Island in Japan, using an SDS/yeast extract pretreatment method (Hayakawa & Nonomura, 1989) and HV agar (Hayakawa & Nonomura, 1987) containing nalidixic acid (20 mg l 21 ). The strain formed thin, flat colonies with sparse, white aerial hyphae on HV agar containing nalidixic acid. The aim of the present study was to determine the taxonomic position of strain TT 00-78T by using a polyphasic approach.The colonial properties of strain TT 00-78T were recorded from colonies grown on a plate containing modified Bennett's agar (Jones, 1949) and incubated for 14 days at 28 uC. Spore motility was examined in hanging drops by means of light microscopy. Gram staining was examined by using Hucker's method (Gerhardt, 1981). Acid-alcoholfastness was examined by using a modified version of the Ziehl-Neelsen method (Gordon, 1967) in which 0.5 % (v/v) sulfuric acid was used for decolorization. The hydrolysis of co...

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Nocardia salmonicida nom. rev., a fish pathogen

Cited by 97 publications

References 23 publications

Williamsia sterculiae sp. nov., isolated from a Chinese medicinal plant

Williamsia sterculiae sp. nov., isolated from a Chinese medicinal plant

Nocardiopsis nikkonensis sp. nov., isolated from a compost sample

Nocardia amamiensis sp. nov., isolated from a sugar-cane field in Japan

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