Nocardia tengchongensis sp. nov., isolated from a soil sample

Abstract

The taxonomic status was determined of two actinomycetes, designated CFH S0057 and CFH S0065, that were isolated from soil samples collected from an extinct volcano in Tengchong county, Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains CFH S0057 and CFH S0065 belong to the genus Nocardia and formed a single clade within this genus. The two isolates were able to grow at 4-45 °C, pH 5.0-7.0 and with a NaCl tolerance up to 5.0% (w/v). The whole-cell hydrolysates were rich in meso-diaminopimelic acid, galactose, arabinose and fructose. Mycolic acids were present. Strains CFH S0057 and CFH S0065 exhibited a menaquinone system with MK-8 (H, ω cyclo), and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified phospholipid. Major fatty acids were C, Summed features 3, C ω9c and C 10-methyl (TBSA). The genomic DNA G + C contents of strains CFH S0057 and CFH S0065 were 65.7 and 66.1 mol%, respectively. The combined genotypic, phenotypic and chemotaxonomic results indicated the isolates are considered to represent a novel species of the genus Nocardia, for which the name Nocardia tengchongensis sp. nov. is proposed. The type strain is CFH S0057 (= KCTC 29485 = JCM 30083).

Nocardia huaxiensis sp. nov., an actinomycete isolated from human skin

“…The strain grew at 10-40 °C (optimum, 28 °C), pH 5.0-12.0 (optimum, 6.0-10.0) and NaCl concentrations of 0-4.0 % (w/v; optimum, 0-2.0 %), which differentiated it from the type strains, N. tengchongensis CFH S0057 T a, Data from Kageyama et al [42]. b, Data from Li et al [43].…”

Nocardia coffeae sp. nov., an endophytic actinobacterium isolated from the root of Coffea arabica (L.)

“…At the present time, the genus Nocardia comprises more than 100 species with validly published names (www.bacterio.net/genus/nocardia). Nocardia species are distributed in various habitats, including soil, decay matter, water and marine sediment [2–4]. Some Nocardia species are reported as opportunistic pathogens in humans.…”

Nocardia acididurans sp. nov., an acid-tolerant actinobacterium isolated from bio-fertilizer of Musa species

“…Nocardia species are generally isolated from various types of soil, e.g. Nocardia tengchongensis [4], Nocardia terrae [5] and Nocardia yunnanensis [6]. Members of the genus Nocardia play an important role in the decomposition and turnover of bio-materials to organic acid in the soil [7,8].…”