Nocardioides daeguensis sp. nov., a nitrate-reducing bacterium isolated from activated sludge of an industrial wastewater treatment plant

Abstract

Nocardioides daeguensis sp. nov., a nitratereducing bacterium isolated from activated sludge of an industrial wastewater treatment plant A Gram-reaction-positive, rod-shaped, non-spore-forming bacterium (strain 2C1-5 T ) was isolated from activated sludge of an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, the closest phylogenetic relatives were the type strains of Nocardioides nitrophenolicus (98.6 % similarity), N. kongjuensis (98.5 %), N. caeni (98.4 %), N. simplex (98.3 %), N. aromaticivorans (98.1 %) and N. ginsengisoli (97.5 %); the phylogenetic distance from other species with validly published names within the genus Nocardioides was greater than 3 %. Strain 2C1-5 T was characterized chemotaxonomically as having LL-2, 6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H 4 ) as the predominant menaquinone and iso-C 16 : 0 , C 16 : 0 and C 17 : 1 v6c as the major fatty acids. The G+C content of the genomic DNA was 74.9 mol%. These chemotaxonomic properties and phenotypic characteristics supported the affiliation of strain 2C1-5 T to the genus Nocardioides. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 2C1-5 T from existing species with validly published names. Therefore, strain 2C1-5 T represents a novel species of the genus Nocardioides, for which the name Nocardioides daeguensis sp. nov. is proposed, with the type strain 2C1-5 T (5JCM 17460The genus Nocardioides was proposed by Prauser (1976) with Nocardioides albus as the type species. Since then, species affiliated with the genus Nocardioides have been actively isolated and studied taxonomically. At the time of writing, the genus comprises 57 species with validly published names, including the recently described species 3These authors contributed equally to this work.

Identification of Nocardioides marmotae sp. nov. and Nocardioides faecalis sp. nov., two new members of the genus Nocardioides

“…MK-8(H 4 ) was the major respiratory quinone detected in strains zg-579 T and zg-536 T , with contents of 68.1 and 88.5 %, respectively. In addition, the two new type strains contained ll -diaminopimelic acid as the diagnostic diamino acid, which was in line with other known species within the genus Nocardioides [20, 27, 28, 50–54]. Strains zg-579 T and zg-536 T shared galactose and ribose in their cell wall, whereas glucose was also found in zg-579 T ; however, data about the cell-wall sugar components of their respective neighbours were not available.…”

Metabolic fate of the14C-labeled herbicide clodinafop-propargyl in a sediment–water system

“…It should how- ever, be mentioned that the genus Nocardioides comprises a large number of closely related species. [44] Previously, different Nocardioides aromaticivorans strains were shown to degrade a number of xenobiotics serving as sole carbon and energy source. [41,45,46] The present data add to Nocardioides aromaticivorans' known capacity for degradation of xenobiotics.…”

Noncontiguous finished genome sequence and description of Nocardioides massiliensis sp. nov. GD13T

“…These Gram-positive, aerobic and cardioform actinomycetes exhibit a high G+C content and are able to develop mycelium [21] , [22] . Currently the genus Nocardioides contains 72 species, including eight described in 2013 and species from the genera Pimelobacter and Arthrobacter that were reclassified in the genus Nocardioides [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] (LPSN, http://www.bacterio.net/ ). Members of the genus Nocardioides are mostly environmental bacteria found in soil or plants.…”